2× SYBR eluhlaza qPCR MIX (Nge-ROX ephezulu)

Iimpawu zemveliso

Le mveliso, i-2 × SYBR eluhlaza qPCR MIX, ifika kwi-tube enye equlethe zonke izinto ezifunekayo kwi-PCR yokukhulisa kunye nokufumanisa, kuquka i-Taq DNA poLymerase, i-SYBR eluhlaza Idayi, i-High ROX Reference Dye, i-dNTPs, i-Mg2 +, kunye ne-PCR buffer.
I-SYBR eluhlaza Idayi yidayi eluhlaza ye-fluorescent ebophelela kwi-DNA ephindwe kabini (i-DNA ephindwe kabini, i-dsDNA) i-double helix minor groove region.SYBR eluhlaza I-fluoresces ibuthathaka kwindawo ekhululekile, kodwa xa ibophelela kwi-DNA ephindwe kabini. , i-fluorescence yayo iphuculwe kakhulu. Oku kwenza ukuba kube lula ukulinganisa ubungakanani be-DNA ephindwe kabini eveliswe ngexesha lokukhulisa i-PCR ngokubona ubungakanani be-fluorescence.
I-ROX isetyenziswa njengedayi yokulungisa ukulungisa ukuguquguquka kwe-fluorescence engahambelani ne-PCR, ngaloo ndlela kuncitshiswa umahluko wendawo. Ukwahlukana okunjalo kunokubangelwa yizinto ezahlukeneyo, ezifana nephutha le-pipette okanye i-evaporation yesampuli. Izixhobo ezahlukeneyo zokulinganisa i-fluorescence zineemfuno ezahlukeneyo ze-ROX, kwaye le mveliso ifanelekile kubahlalutyi be-fluorescence quantification efuna ukulungiswa kwe-ROX ephezulu.