2 × Syb Green qpc qpc (nge-rox ephezulu)

I-2 × Syb Green qpc qpc qpc (nge-rox ephezulu) iboniswe umfanekiso

Inkcazo emfutshane:

Le mveliso, 2 × 2 × Syb Green QPR Umxube, uza kwityhubhu enye equlethe onke amacandelo e-PCR APE, i-DNGB iDye iDye, i-DNTPS, MG2 +, kunye ne-PCR Buff.

QHAGAMSHELANA NATHI

Iinkcukacha zemveliso

Iimpawu zeMveliso

Le mveliso, 2 × 2 × Syb Green QPR Umxube, uza kwityhubhu enye equlethe onke amacandelo e-PCR APE, i-DNGB iDye iDye, i-DNTPS, MG2 +, kunye ne-PCR Buff.
I-Syb Green i Dye yidayi eluhlaza ye-fluorescence fluorescent ebopha i-DNA ephindiweyo Oku kwenza ukuba kwenzeke ukuba kwenzeke ukuba i-DNA ye-DNA eveliswe kabini eveliswa ngexesha le-PCR ye-PCR ngokufumana ubukhulu be-fluorescence.
I-ROX isetyenziswa njengedayisi yokulungisa ukulungisa utshintsho lwe-fluorescence olungahambelani kwi-pcr, ngaloo ndlela kuncipha umahluko wendawo. Ukwahluka okunjalo kunokubangelwa zizinto ezahlukeneyo, ezinje ngempazamo ye-pipette okanye isampulu yokuphuma. Izixhobo ezahlukeneyo zokufakelwa kwe-Fluorescence zineemfuno ezahlukileyo zeRox, kwaye le mveliso ilungele abahlalutyo lwe-Fluorescence Fluorescence efuna ukulungiswa kweRox ephezulu.

Izinto eziphambili

Num.	Ukudibanisa	Ukucaciswa	Ubungakanani	Izinto eziphambili
1	2 × Syb Green qpc qpc (nge-rox ephezulu)	1ml	5	I-DNTPS, i-tris buffers, iRox ephezulu, i-Taq DNA Polymerase, Syb Green i Dyes, MG2 +