2 × SYBR green qPCR MIX （Ene-ROX Ephezulu）

Iimpawu zemveliso

Le mveliso, i-2×SYBR green qPCR MIX, iza kwityhubhu enye equlethe zonke izinto ezifunekayo ukuze kuphuculwe kwaye kufunyanwe i-PCR, kuquka i-Taq DNA poLymerase, idayi ye-SYBR green I, idayi ye-High ROX Reference, i-dNTPs, i-Mg2+, kunye ne-PCR buffer.
Idayi eluhlaza ye-SYBR yidayi eluhlaza ekhanyayo ebopha kwi-DNA enemicu emibini (i-DNA enemicu emibini, i-dsDNA) indawo ye-double helix minor groove. I-SYBR green I ikhanya kancinci kwimeko ekhululekileyo, kodwa xa ibopha kwi-DNA enemicu emibini, ukukhanya kwayo kuyaphucuka kakhulu. Oku kwenza kube nokwenzeka ukubala ubungakanani be-DNA enemicu emibini eveliswayo ngexesha lokwandiswa kwe-PCR ngokufumanisa ubunzulu be-fluorescence.
I-ROX isetyenziswa njengedayi yokulungisa ukuguquguquka kwe-fluorescence okungahambelaniyo ne-PCR, ngaloo ndlela kunciphisa umahluko wendawo. Umahluko onjalo unokubangelwa zizinto ezahlukeneyo, ezifana nempazamo yepipette okanye ukufuma kwesampuli. Izixhobo ezahlukeneyo zokulinganisa i-fluorescence zinemfuno ezahlukeneyo ze-ROX, kwaye le mveliso ifanelekile kwii-analyzers ze-fluorescence quantification ezifuna ukulungiswa kwe-High ROX.