I-MagPure Plasmid DNA Yokucoca Ikhithi

Inkcazo emfutshane:

Le khithi isebenzisa inkqubo ethile ye-buffer ephuhliswe kakuhle nelungiselelweyo kunye nee-magnetic beads ezibopha ngokukodwa kwi-DNA, ezinokubopha ngokukhawuleza, zifunxe, zahlukanise kwaye zicoce ii-nucleic acids. Ifanelekile kakhulu ukwahlula ngokukhawuleza nangokufanelekileyo kunye nokucoca i-plasmid DNA kwi-0.5-2mL (ehlala i-1-1.5mL) ulwelo lwebhaktiriya, ngelixa isusa iintsalela ezifana neeproteni kunye nee-ion zetyuwa. Ngokuxhasa ukusetyenziswa kweBigfish Magnetic Bead Nucleic Acid Extractor, ifanelekile kakhulu ekukhupheni ngokuzenzekelayo ubungakanani obukhulu beesampuli. I-plasmid DNA ekhutshiweyo inobunyulu obuphezulu kunye nomgangatho olungileyo, kwaye ingasetyenziswa ngokubanzi kwiimvavanyo ezisezantsi ezifana nokugaya i-enzyme, ukubopha, ukuguqulwa, i-NGS, njl..

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Intshayelelo emfutshane

Iimpawu zeMveliso

Umgangatho olungileyo:Hlukanisa kwaye ucoce i-plasmid DNA kwisisombululo sebhaktiriya esingu-0.5-2mL ngemveliso ephezulu kunye nobumsulwa obuhle.

Ngokukhawuleza kwaye kulula:Yonke le nkqubo ayifuni ukujikeleziswa kwe-centrifugation okanye imisebenzi yokucoca, nto leyo eyenza ifaneleke ekukhupheni ubungakanani obukhulu beesampuli.

Ikhuselekile kwaye ayinabungozi:Akukho mfuneko yee-reagents ze-organic ezinobungozi ezifana ne-phenol/chloroform.

IyalungisekaIzixhobo

Intlanzi enkulu: BI-FEX-32E, I-BFEX-32, I-BFEX-96E, BFEX-16E

Inkcazo yemveliso

ImvelisoName	Ikati. Hayi.	Ukupakisha
I-MagPure Plasmid DNA Purification Kit (iphakheji ezaliswe kwangaphambili)	BFMP09R	32T
I-MagPure Plasmid DNA Purification Kit (iphakheji ezaliswe kwangaphambili)	BFMP09R1	40T
I-MagPure Plasmid DNA Purification Kit (iphakheji ezaliswe kwangaphambili)	BFMP09R96	96T
I-RNaseA(IsiNgesi)thenga）	BFRD017	1ml/ ityhubhu(IsiNgesi)10mg/ml）