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I-MagPure Plasmid DNA Yokucoca Ikhithi
Intshayelelo emfutshane
Le khithi ithatha inkqubo ethile ye-buffer ephuhlisiwe kunye neyongezelelweyo ekhethekileyo kunye namaso amagnetic abophe ngokukodwa kwi-DNA, enokuthi ibophe ngokukhawuleza, ibhengeze, ihlukanise kwaye ihlambulule i-nucleic acids. Ifaneleke kakhulu ngokukhawuleza nangokufanelekileyo ukwahlula kunye nokucoca i-plasmid DNA ukusuka kwi-0.5-2mL (ngokuqhelekileyo i-1-1.5mL) ulwelo lwebhaktheriya, ngelixa ususa iintsalela ezifana neeprotheni kunye ne-ion zetyuwa. Ngokuxhasa ukusetyenziswa kweBigfish Magnetic Bead Nucleic Acid Extractor, ilungele kakhulu ukutsalwa okuzenzekelayo kobukhulu besampulu. I-DNA ye-plasmid ekhutshweyo inobunyulu obuphezulu kunye nomgangatho omhle, kwaye ingasetyenziselwa ngokubanzi kwiimvavanyo ezisezantsi ezifana nokugaya i-enzyme, i-ligation, inguqu, i-NGS, njl..
Iimpawu zeMveliso
Umgangatho olungileyo:Yahlula kwaye uhlambulule i-plasmid DNA kwisisombululo sebhaktiriya esine-0.5-2mL enesivuno esiphezulu kunye nokucoceka okulungileyo..
Ngokukhawuleza kwaye kulula:Yonke le nkqubo ayifuni i-centrifugation ephindaphindiweyo okanye imisebenzi yokucoca, iyenza ilungele ukutsala iisampulu ezinkulu..
Ikhuselekile kwaye ayinayo ityhefu:Akukho mfuneko yeereagents ezinetyhefu ezifana nephenol/chloroform.
IyaguqukaIzixhobo zomculo
Intlanzi enkulu: BFEX-32E, BFEX-32, I-BFEX-96E, BFEX-16E
Ukucaciswa kwemveliso
| ImvelisoName | Ikati. Hayi. | Ukupakisha |
| I-MagPure Plasmid DNA Purification Kit (iphakheji ezaliswe kwangaphambili) | I-BFMP09R | 32T |
| I-MagPure Plasmid DNA Purification Kit (iphakheji ezaliswe kwangaphambili) | I-BFMP09R1 | 40T |
| I-MagPure Plasmid DNA Purification Kit (iphakheji ezaliswe kwangaphambili) | I-BFMP09R96 | 96T |
| RNaseA(ukuthenga） | BFRD017 | 1ml / ityhubhu(10mg/ml） |
Iisetingi zabucala