Isixhobo sokubona iNyukleyi yeNyukleyi yeNyukleyi yeNyukleyi (iFluorescent RT-PCR) hsif gi B uoh zg naH . dt L,. oC hcet- oi B

Inkcazo emfutshane:

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimbonakalo

Uvakalelo oluphezulu: Umda woKuchongwa (LoD) ＜5 × 102 iikopi/ml

Ngolawulo lwangaphakathi: Jonga inkqubela phambili yovavanyo kwaye uphephe iziphumo ezingezizo ezilungileyo

Ifanelekile kwizixhobo ezahlukeneyo: ABI 7500/7500 FAST; Roche LightCycler480; BioRad CFX96; IBigFish-BFQP 48/96 yethu

Ikhawuleza kwaye ilula: I-reagent exutyiweyo kwangaphambili kulula ukuyisebenzisa, abathengi bafuna nje ukongeza i-enzyme kunye netemplate. Ikhithi yokukhupha i-nucleic acid yeBigfish ihambelana kakuhle nolu vavanyo. Ngokusebenzisa umatshini wokukhupha ozenzekelayo opheleleyo, ikhawuleza ukucubungula iisampulu ezininzi.

Ukhuseleko lwe-bio: I-Bigfish ibonelela ngeSampuli yoLungiso oluSindayo ukuze ikhawulezise ukungasebenzi kwentsholongwane ukuqinisekisa ukhuseleko lwabaqhubi

Isatifikethi se-CE-IVD

Igama leMveliso	Ikati. Hayi.	Ukupakisha	Amanqaku
Isixhobo sokubona iNyukleicAcid yeMonkeypox Virus (iFluorescent RT-PCR)	I-BFRT39S	25T	Uvakalelo oluphezulu, Ifanelekile kwiisampuli ezibuthathaka
Isixhobo sokubona iNyukleicAcid yeMonkeypox Virus (iFluorescent RT-PCR)	I-BFRT39M	50T	Uvakalelo oluphezulu, Ifanelekile kwiisampuli ezibuthathaka

Izicelo

Isixhobo sokufumanisa i-nucleic acid yentsholongwane yeMonkeypox (iFluorescence RT-PCR) singasetyenziselwa ukufumanisa i-nucleic acid yentsholongwane yemonkeypox, sisetyenziselwa ukuxilongwa okuncedisayo kunye nophando lwe-epi-demiological losulelo lwentsholongwane yemonkeypox, olufanele i-CDC, izibhedlele, ilebhu yezonyango yomntu wesithathu, iziko lovavanyo lomzimba kunye nezinye iilebhu zeklinikhi.

Ngaphambili: Isixhobo sokufumanisa intsholongwane yehagu yaseAfrika (indlela ye-fluorescence PCR)

Okulandelayo: Isampuli yasimahla yoMatshini we-Biobase Quantitative PCR oneeFomati zeBloko ezingama-96

Bhala umyalezo wakho apha uze uwuthumele kuthi