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Kwimimandla yeukuxilongwa kweklinikhi kwi-vitro (IVD), i-genotyping, kunye nophando lwe-molecular, iisampulu zomlomo—ezifanaiiswabhu zomlomo, iiswabhu zomqala, kunye namathe—zisetyenziswa kakhulu kuvavanyo lwe-nucleic acid ngenxa yezizathu zazoukuqokelela okulula, indalo engangenisi ntlungu, kunye nenkqubo yokuthatha iisampulu engenabuhlunguNangona kunjalo, iisampulu zomlomo zihlala ziqulatheubungakanani obuncinci be-nucleic acidskwaye zihlala zingcoliswa yiiiproteni kunye nezinye izinto ezingcolileyoIindlela zemveli zokukhupha zidla ngokuba neengxakiimisebenzi enzima, ukusebenza kakuhle okuphantsi, kunye nokusetyenziswa kwee-reagents ezinobungozi, nto leyo enokubeka emngciphekweni kakhulu ukuchaneka kunye nokuzinza kwezicelo ezisezantsi ezifanaI-PCR/qPCR kunye nolandelelwano lwesizukulwana esilandelayo (NGS).
IIkhithi yokuKhutshwa kweDNA ye-BFMP06 Magnetic Bead-Based Oral Swab Genomic DNA, yaphuhliswa nguI-Hangzhou Bigfish FeiXu Biotechnology, inikezaisisombululo esikhuselekileyo, esisebenzayo, nesithembekileyoyokukhupha i-DNA yesampuli yomlomo. Ngoyilo lwayo lobugcisa olutsha kunye nemigangatho yokusebenza engqongqo, le khithi ibe sisixhobo esithembekileyo kwiilabhoratri zeklinikhi nezophando.
Ikhithi ye-BFMP06 yakhelwe malungainkqubo ye-buffer eyenziwe ngcono kakhuluzidityaniswe neIibhile zemagnethi ze-hydroxyl ezithile ze-DNA, yenze umsebenzi wokucoca i-nucleic acid osebenza kakuhle kakhulu. Emva kokuba isampuli iphoswe kwi-lysis buffer, izinto zeseli ziyaphazamiseka kwaye ii-nucleic acid ziyakhutshwa. Amaqela asebenzayo kumphezulu weentsimbi zemagnethi akhetha ukubopha i-DNA ekhululekileyo, enze i-stableii-magnetic bead–DNA complexes.
Phantsi kwentsimi yamagnetic yangaphandle, ezi complexes zidlula kuzoamanyathelo amabini achanekileyo okuhlambaukususa ngokupheleleyo iiproteni, iityuwa, kunye nezinye izinto ezingcolisayo. Okokugqibela,i-DNA ye-genomic ecocekileyo kakhuluisuswa ngokufanelekileyo kusetyenziswa i-elution buffer.
Ingcaciso yeMveliso
Le mveliso isebenzisa inkqubo ye-buffer eyenzelwe ngokukodwa kwaye ilungiselelwe kunyeiibhere zemagnethi ezibopha i-DNA ngokukodwa, okuvumela ukufunxwa ngokukhawuleza, ukwahlulwa, kunye nokucocwa kwee-nucleic acids. Ifanelekile kakhulu kwiUkwahlulwa ngokukhawuleza nangokufanelekileyo kwe-DNA ye-genomic kwiiswabhu zomlomo, iiswabhu zomphimbo, kunye neesampuli zamathe, ngelixa isusa ngempumelelo iiproteni eziseleyo kunye neetyuwa.
Xa isetyenziswa kunyeIzixhobo zokukhupha i-nucleic acid ezisekelwe kwi-Bigfish FeiXu magnetic bead, le khithi ifanelekile kwiukukhupha ngokuzenzekelayo okuphezuluI-DNA ye-genomic ecocekileyo yeyoubunyulu obuphezulu kunye nomgangatho ogqwesileyo, okwenza ifaneleke kwiintlobo ngeentlobo zezicelo ezisezantsi, kuqukaI-PCR/qPCR kunye ne-NGS.
Iimpawu zeMveliso
Umgangatho ophezulu
Iyahlukanisa kwaye icoca i-DNA ye-genomic ngokufanelekileyo kwiiiswabhu zomlomo, iiswabhu zomqala, kunye namathe, ukuhambisaisivuno esiphezulu kunye nobunyulu obuphezulu.
Iyakhawuleza kwaye ilula
Akukho mfuneko yokucoca ngokuphindaphindiweyo okanye amanyathelo okucoca nge-vacuum. Iyahambelana nezixhobo zokukhupha ezizenzekelayo, nto leyo eyenza ukuba ilungele ukusetyenziswaukucutshungulwa kwesampulu enkulu.
Ikhuselekile kwaye ayinatyhefu
Akukho mfuneko yee-reagents ezinobungozi ze-organic ezifanai-phenol okanye i-chloroform.
Izixhobo Ezihambelanayo
Intlanzi enkulu iFeiXu BFEX-16E
I-BFEX-32
I-BFEX-32E
I-BFEX-96
Iziphumo zovavanyo
Iisampuli zeswab yomlomo (ezifakwe kwiIsisombululo sokugcina esingu-400 μLkunye neesampuli zamathe (Isisombululo sokugcina esingu-200 μL samathe + isisombululo sokugcina esingu-200 μL) zicutshungulwe kusetyenziswaI-Bigfish FeiXu Oral Swab Genomic DNA Purification Kit. I-DNA ayifumanekangaI-70 μL elution bufferkwaye yahlalutywa ngui-electrophoresis yejeli ye-agarose, njengoko kubonisiwe kumfanekiso ongezantsi.
M: Uphawu lwe-DNA (2K Plus II)
Iinkcukacha zeMveliso
Ixesha leposi: Jan-15-2026
Izicwangciso zabucala