I-Bigfish Oral Swab DNA Purification Kit: Isisombululo Esisebenza Kakhulu Sokukhupha I-DNA Kwisampuli Yomlomo

Kwimimandla yeukuxilongwa kweklinikhi kwi-vitro (IVD), i-genotyping, kunye nophando lwe-molecular, iisampulu zomlomo—ezifanaiiswabhu zomlomo, iiswabhu zomqala, kunye namathe—zisetyenziswa kakhulu kuvavanyo lwe-nucleic acid ngenxa yezizathu zazoukuqokelela okulula, indalo engangenisi ntlungu, kunye nenkqubo yokuthatha iisampulu engenabuhlunguNangona kunjalo, iisampulu zomlomo zihlala ziqulatheubungakanani obuncinci be-nucleic acidskwaye zihlala zingcoliswa yiiiproteni kunye nezinye izinto ezingcolileyoIindlela zemveli zokukhupha zidla ngokuba neengxakiimisebenzi enzima, ukusebenza kakuhle okuphantsi, kunye nokusetyenziswa kwezinto ezinobuthi, nto leyo enokubeka emngciphekweni kakhulu ukuchaneka kunye nokuzinza kwezicelo ezisezantsi ezifanaI-PCR/qPCR kunye nolandelelwano lwesizukulwana esilandelayo (NGS).

IIkhithi yokuKhutshwa kweDNA ye-BFMP06 Magnetic Bead-Based Oral Swab Genomic DNA, yaphuhliswa nguI-Hangzhou Bigfish Biotechnology, inikezaisisombululo esikhuselekileyo, esisebenzayo, nesithembekileyoyokukhupha i-DNA kwisampuli yomlomo. Ngoyilo lwayo lobugcisa olutsha kunye nemigangatho yokusebenza engqongqo, le khithi ibe sisixhobo esithembekileyo kwiilabhoratri zeklinikhi nezophando.

Ikhithi ye-BFMP06 yakhelwe malungainkqubo ye-buffer eyenziwe ngcono kakhuluzidityaniswe neIibhile zemagnethi ze-hydroxyl ezithile ze-DNA, yenze umsebenzi wokucoca i-nucleic acid osebenza kakuhle kakhulu. Emva kokuba isampuli iphoswe kwi-lysis buffer, izinto zeseli ziyaphazamiseka kwaye ii-nucleic acid ziyakhutshwa. Amaqela asebenzayo kumphezulu weentsimbi zemagnethi akhetha ukubopha i-DNA ekhululekileyo, enze i-stableii-magnetic bead–DNA complexes.

Phantsi kwentsimi yamagnetic yangaphandle, ezi complexes zidlula kuzoamanyathelo amabini achanekileyo okuhlambaukususa ngokupheleleyo iiproteni, iityuwa, kunye nezinye izinto ezingcolisayo. Okokugqibela,i-DNA ye-genomic ecocekileyo kakhuluisuswa ngokufanelekileyo kusetyenziswa i-elution buffer.

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