Uvavanyo lwe-DNA Metiety Ukudityaniswa nee-smartphones zokuhlolwa kwe-tuumurs kunye ne-leukemia ehlolwe ngokuchaneka kwe-90.0%!

Ukufunyanwa kwangoko komhlaza ngokusekwe kwi-biopsy ye-biopsy yindlela entsha yokuchongwa komhlaza kule minyaka i-US ye-US, ngenjongo yokufumana umhlaza kwangoko okanye kwizilonda zokhuseleko. Isetyenziswe ngokubanzi njenge-biomarker ye-novelmer ekufumaneni isifo ngokwamatyeni ohlukeneyo, kubandakanya nomhlaza wemiphunga, i-gestrointenal tumes, i-gliomas kunye ne-gynecological tumerulogical sume.

Ukuvela kwamaqonga okuchonga imbonakalo ye-methylity (Methylscape) ii-biomarkers inakho ukuphucula ukufunwa okukhoyo kwangoko kumhlaza, ukubeka izigulana kwiqonga elinokunyathela.

Kutshanje, abaphandi baphuhlise iqonga elilula nelithe ngqo kwi-methylity ye-methyyrecape ngokusekwe kwi-ENGSEATATICE YENKCAZO YOKUGQIBELA EYENZIWEYO I-biosernser esekwe kwi-Smartphone eyenza ukuba kuhlolwe ngokukhawuleza amathumba amaninzi. Ukuhlolwa kwangoko kwe-leukemia kunokwenziwa ngaphakathi kwemizuzu eli-15 emva kokukhutshwa kwe-DNA kwisampulu yegazi, ngokuchaneka kwe-90.0%. Isihloko senqaku sikufunyanwa ngokukhawuleza kwe-DNA yomhlaza kwiGazi lomntu lisebenzisa i-Auns-i-Auntes ePsyse-i-Smart-enikwe i-smarkcophoone.

Umzobo 1. Iqonga elilula nelila ngokukhawuleza lovavanyo lomhlaza nge-cyst / Aunsps zenziwe ngamanyathelo amabini alula.

Oku kubonisiwe kumzobo 1. Okokuqala, isisombululo se-ACEACE sasisetyenziselwa ukunyibilikisa iziqwenga zeDNA. UCyst / Aunep wathunyelwa kwisisombululo esixubeneyo. I-DNA eqhelekileyo nengalunganga ineepropathi zobume be-methylity, ezikhokelela kwiziqwenga zeDNA kunye neepateni ezahlukeneyo zokuzibandakanya. I-DNA eqhelekileyo ixhamla ngokukhululekileyo kwaye ekugqibeleni ixhamla i-cyst / i-Auns, ekhokelela kuhlobo olutshintshiweyo lwe-cyst / i-Auns, ukuze utshintsho kumbala obomvu lubonwa ngamehlo. Ngokwahlukileyo, iprofayili ye-methyty eyahlukileyo yeDNA yeDela ikhokelela ekuvelisweni kwamaqela amakhulu ee-DNA.

Imifanekiso engama-96-ipleyiti ithathiwe isetyenziswa usebenzisa ikhamera ye-smartphone. I-DNA yomhlaza yayilinganiswe yi-smartphone exhotyiswe ngomatshini ekufumaneni ngokuthelekiswa neendlela ezisekwe kwi-spectrascopy.

Ukuhlolwa komhlaza kwiisampulu zegazi zokwenyani

Ukwandisa isixhobo seqonga leenqwana, abaphandi bafaka i-Ensor Iipateni ze-methyyration kwiziza ze-CPG zisingisa. Phantse zonke iintlobo zomhlaza, iinguqu kwi-DNA METHYIT kwaye ke kwiintlobo zemfuza eziphakamisa i-tuculogenesis ziye zaqwalaselwa.

Njengomzekelo womhlaza onxulumene ne-DNA Metimeration, abaphandi basebenzisa iisampulu zegazi ezivela kwizigulana ze-leukemia kunye nolawulo olusempilweni ukuphanda ngempumelelo kwe-MetYyemac Doulers. Lo mhlaba we-methylith biomarcar biomarker ayikasebenzi nje kwiindlela zokutshekishwa kwe-leukemia okhethekileyo, kodwa ikwabonisa ukuba kuqaliswe kwangoko ukubonwa kwangoko kuluhlu olubanzi lomhlaza usebenzisa olu lwaza olulula nolithuli.

I-DNA kwiisampulu zegazi ukusuka kwizigulana ezingama-31 ze-leukemia kunye nabantu abasempilweni abayi-12 bahlalutywa. Njengoko kubonisiwe kwiBhokisi yeBhokisi ye-2A, ukuDibana ngokuLungelelanisa iisampulu zomhlaza (δA650 / 525) yayiphantsi kune-DNA kwiisampulu eziqhelekileyo. Oku ikakhulu ngenxa ye-hydrophobizetion ye-hydrophobility ekhokelela ekunyukeni kweDNA yomhlaza, ethintela ukudityaniswa kwe-cyst / Aunep. Ngenxa yoko, ezi zibano zasasazeka ngokupheleleyo kwizimbo zomhlaza, ezikhokelele ekusasazeni okwahlukileyo kwe-cysy / Auns e-Aggred kwi-DNA ye-DNA yesiqhelo nemhlaza. I-ROC ivule emva koko iveliswe ngokwahluka komda ukusuka kwixabiso elincinci le-δA650 / 525 ukuya kwixabiso eliphezulu.

Umzobo 2. (a) Ixabiso lokulinganisa lokulinganisa le-cyst / Auns Izisombululo ezibonisa ubukho bezinto eziqhelekileyo (eziluhlaza) kunye nomhlaza (obomvu) i-DNA phantsi kweemeko ezifanelekileyo

(Da650 / 525) yeeplani zebhokisi; (b) Uhlalutyo lweRoC kunye noVavanyo lweemvavanyo zokuxilonga. (c) Imatrix yediski yokuchonga izigulana eziqhelekileyo nezinomhlaza. .

Njengoko kubonisiwe kwi-2b 2B, le ndawo iphantsi kwe-roc curve (i-AUC = 0.9274) ifunyenwe kwi-insor ephucukileyo ibonise ubuntununtunu kunye nobumnene. Njengoko kunokubonwa kwiBhokisi yeBhokisi, eyona ndawo iphantsi emele iqela eliqhelekileyo le-DNA ayihambelani neyona ndawo iphakamileyo imele iqela leDNARR; Ke ngoko, i-retrogus yelogo yayisetyenziselwa ukwahlula phakathi kwamaqela aqhelekileyo kunye nomhlaza. Ngenxa yeseti yezinto ezizimeleyo eziguquguqukayo, iqikelela ukuba kunokwenzeka komcimbi owenzekayo, onjengomhlaza okanye iqela eliqhelekileyo. I-ranges eguquguqukayo phakathi kwe-0 ne-1. Isiphumo ke sinokwenzeka ke. Sigqibe kwelokuba ingenisi somhlaza (p) esekwe kwi-δA650 / 525 ngolu hlobo lulandelayo.

Apho b = 5.3533, W1 =6.965. Kwisampulu yokwahlula-hlulwa, amathuba okuba ngaphantsi kwe-0.5 ibonisa isampulu yesiqhelo, ngelixa inokwenzeka ye-0.5 okanye ngaphezulu ibonisa isampulu yomhlaza. Umzobo 2c ubonakalisa i-matrix yediski eveliswe kwikhefu-iyona-yodwa-iyodwa yokuqinisekiswa, eyayisetyenziselwa ukuqinisekisa uzinzo lwendlela yokwazisa. Umzobo 2d ushwankathela uvavanyo lovavanyo lwendlela yendlela, kubandakanya nemvakalelo, ukuqina, ixabiso elifanelekileyo (i-PPV) kunye nexabiso elifanelekileyo (NPV).

I-Smartphoners esekwe kwi-biosens

Ukuqhubela phambili kuvavanyo lwesampulu ngaphandle kokusetyenziswa kwee-spectrophotometers, abaphandi basebenzise ubukrelekrele (i-AI) ukutolika umbala wesisombululo kwaye ukwahlula phakathi kwabantu abaqhelekileyo nabanomvuzo. Ngenxa yoku, umbono wekhompyuter wawusetyenziselwa ukuguqulela umbala wesisombululo seCyst / Auns kwi-DNA eqhelekileyo (i-pumele) okanye iDNA ye-Free (iRed) usebenzisa ikhamera yefowuni yefowuni. Ubukrelekrele bokwenyama bunokunciphisa iindleko kwaye kuphuculwe ukufikeleleko lokutolika umbala wesisombululo se-nanopricals, kwaye ngaphandle kokusetyenziswa kwezinto ze-SORKINOP Okokugqibela, iimodeli ezimbini zokufunda umatshini, kubandakanya ihlathi elingenamsebenzi (i-RF) kunye noMatshini weVector yeNkcazo (SVM) baqeqeshwa ukwakha iimodeli. Zombini iimodeli ze-RF kunye ne-SVM zichaza ngokufanelekileyo iisampulu njengezona zilungileyo nezingalunganga ngokuchaneka kwe-90.0%. Oku kuphakamisa ukuba ukusetyenziswa kobukrelekrele bezinto zokwenziwa kwi-biosensing esekwe kwifowuni kusenokwenzeka.

Umzobo 3. (a) Iklasi ekujoliswe kuyo isisombululo ebhalwe ngexesha lokulungiselela isampulu yenqanaba lokufumana umfanekiso. (b) Umzekelo umfanekiso othathwe ngexesha lokufumana umfanekiso. .

Sebenzisa i-cyst / Auneps, abaphandi baphuhlise ngempumelelo iqonga elilula lokuhlolwa kwe-methylity ye-methylith kunye nejensor ekwaziyo ukwahlula i-DNA ye-DNA xa usebenzisa iisampulu zegazi ze-leukemia. Isinyithi esiphuhlisiweyo sibonisa ukuba i-DNA ikhutshwe kwiisampulu zegazi zokwenyani yakwazi ukukhawulezisa kwaye ifumana iindleko ezibonakalayo kwi-DNA yomhlaza (i-3.3% ye-95.3%. Ukuqhubela phambili kuvavanyo lwesampulu ngokuphelisa isidingo se-spectrophotometer, ukufunda ngomatshini kwasetyenziswa ukutolika umbala wesisombululo kunye nokuchaneka kwefowuni esebenzisa i-90.0%.

Isalathiso: Doi: 10.1039 / D2RA0572E