Jonga ukuguquguquka komjikelo we-thermal kuphando

Abajikelezi bomzimba, ekwabizwa ngokuba ngabatshini boomatshini, zizixhobo ezibalulekileyo kwimolekyuli yemolekyuli kunye nophando lwemfuza. Ezi zixhobo zisetyenziselwa ukwanda kwe-DNA kunye ne-RNA kwi-Polymerase Chain impendulo (i-PCR). Nangona kunjalo, ukutshintsha komjikelo webhulori akupheleli kwizicelo ze-PCR. Kweli nqaku, siza kuphonononga iindlela ezahlukeneyo zokuhamba komjikelo osetyenziswa kuphando kunye nokubaluleka kwabo ekuqhubeleni phambili ulwazi lwezenzululwazi.

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Umsebenzi ophambili weumjikelezo we-thermalKukwenza u-PCR of pulpha, obaluleke kakhulu kwizicelo zebhayoloji zemolekyuli. Ngokuthobela i-DNA okanye isampulu ye-RNA ukuya kuthotho lobushushu, abajikelezayo be-irmal bakhuthaza ubumo, ukungqubana, kunye nolwandiso lwe-nucleid strands, kukhokelela ekunikezelweni kolandelelwano oluthile lwethagethi. Le nkqubo ibaluleke kakhulu kuhlalutsho lwemfuza, intetho yesifundo se-tele, kunye nokufumana iiarhente zosulelo.

I-2. I-PCR ye-PCR (QPCR)

Ukongeza kwi-PCR, imijikelezo ye-thermal isetyenziselwa ubungakanani be-PCR okanye i-QPCR, ivumela inani leethagethi ze-sukuid kwisampulu. Ngokufaka i-dyscent ye-fluorescent okanye i-proodes, abajikelezayo be-thermas banokulinganisa ukuqokelelwa kweemveliso ze-PCR ngexesha lokwenyani, ukubonelela ngokuqonda okuxabisekileyo kumanqanaba enkcazo, umthwalo wentsholongwane, kunye nokwahluka kwentsholongwane.

3. Ukubuyiswa kwe-PCRCR (RT-PCR)

Abajikelezayo be-thermas badlala indima ebalulekileyo ekubuyiselweni ngasemva prcr, ubuchule obuguqula i-RNA kwi-DNA eguquguqukayo (i-CDNA) yokukhulisa kamva. Le ndlela ibaluleke kakhulu ekufundeni intetho yesifo, i-RNA intsholongwane, kwaye iipateni ze-MRNA. Umjikelezo we-thermal onolawulo lobushushu oluchanekileyo lubalulekile kwimpumelelo yeemvavanyo ze-RT-prc.

I-4. Idijithali yedijithali

Inkqubela phambili kwitekhnoloji yomjikelo we-thermal ikhokelele kuphuhliso lwedijithali yedijithali, indlela enobuthathaka kakhulu yokuxabana ngokupheleleyo kwee-acid zenyukliya. Ngokwahlula i-PCR yokuphendula ibe ngamawakawaka okuqala, umjikelo we-thermal unokuchonga ngokuchanileyo imolekyuli yemolekyuli, enza isixhobo sedijithali i-PRCIT isixhobo esibonakalayo sokufumana i-raare yokuguqula kunye nokukopa kohlaziyo lweerejista zenani.

5. Ukulungiselela amathala eencwadi elandelayo

Abajikelezi be-thermal yinxalenye yenkqubo yokulungiselela ithala leencwadi kwizicelo ezilandelayo zokulandelelana (ii-NG). Ngokwenza i-progitions esekwe kwi-DNA, imijikelezo ye-irmal yenza ukuba ulwakhiwo lwelayibrari ukuqala kwezinto ezilinganiselweyo, ukuvumela abaphandi ukuba bahlalutye lonke i-genome, i-pigepriptome.

I-6. Iiprotein zobunjineli kunye ne-mutagenesis

Ukongeza kwi-nucleid i-acid, imijikelezo ye-thermal isetyenziswa kwizifundo zobunjineli kunye nezifundo zeMutagedos. I-STE-Refred MonDodnesis, i-Protein Polly, kwaye yabhekiswa kwiindlela zokuvela kwendalo rhoqo kwiindlela ezisekwe kwi-PCR, kunye ne-thermal of the the Apciold Lit kunye neRineres ePhezulu kwaye ibaluleke kakhulu ekufumaneni iziphumo ezichanekileyo nezibuyisiweyo.

7. Ukusingqongileyo kunye noKhuseleko loKhuseleko loKutya

Abajikelezayo be-thermal ikwasetyenziswa kuvavanyo lwendalo kunye noKhuseleko loKutya, ngakumbi ukufunyanwa kweentsholongwane zentsholongwane, izinto eziguqulweyo zemfuza (GMOs) kunye ne-tepogenen enemifanekiso. Uvavanyo olusekwe kwi-PRREALS lwenza ukuba i-thermal ivumela ukukhawulezisa kunye nokuchongwa okuthile kweempangelo, ukuqinisekisa ukhuseleko kunye nomgangatho wokutya kunye neesampulu zokusingqongileyo.

Isishwankathelo,Abajikelezi bomzimbaNgaba izixhobo ezingabonakaliyo kwi-molecular biology kunye ne-genetics, zibonelela ngoluhlu olubanzi lwezicelo ze-PCRC ze-PCR. Ukutshintsha kwabo kunye nokuchaneka kubenza baluleke kuvavanyo oluvela kulwaliso lwenkcazo yendalo. Njengoko itekhnoloji iyaqhubeka ukuqhubela phambili, abanqolobi bebhungana banokudlala indima ebaluleke ngakumbi ekuqhubeni ukufunyanwa kwesayensi kunye nezinto ezintsha.