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Inkqubela phambili enxulumeneyo
Ngokutsho kwe-Ofisi yoLwazi lweSebe lezoLimo kunye neendawo zasemaphandleni, ngo-Agasti 2018, isibetho sehagu sase-Afrika senzeke kwiSithili esitsha saseShenbei, kwisixeko saseShenyang, kwiPhondo laseLiaoning, isibetho sokuqala sehagu saseAfrika eChina. Ukusukela nge-14 kaJanuwari 2019, isibetho seehagu zase-Afrika senzeke kumaphondo angaphezu kwama-20 e-China, sabulala iihagu ezingama-916000, ezibangela inkxalabo yoluntu.
I-African Swine fever (ASF)
I-ASF(African Swine fever) sisifo esosulelayo esimandla esibangelwa yi-African swine fever virus (ACFV) esosulela iihagu zasekhaya kunye neehagu zasendle (iihagu zasendle zase-Afrika, iihagu zasendle zaseYurophu, njl. njl.). Umbutho wehlabathi wempilo yezilwanyana (OIE) uyidwelise njengesifo sezilwanyana esixelwe ngokomthetho, esikwaluhlobo lobhubhani wezilwanyana i-China igxile ekuthinteleni.
Iimpawu zesi sifo
Iimpawu zeklinikhi yifiva (ukuya kuma-40 ~ 42 ℃), i-tachycardia, i-dyspnea, ukhohlokhohlo oluyinxenye, i-serous okanye i-mucinous purulent secretion emehlweni nasempumlweni, i-cyanosis yesikhumba, ukopha okucacileyo kwezintso, i-lymph node kunye ne-mucosa yesisu. Iimpawu zekliniki ze-African classical swine fever ziyafana nezo ze-classical swine fever, ezinokuthi zifunyanwe kuphela ngokujongwa kwilabhoratri.
Isisombululo kwi-African classical swine fever
1. Ukulungiswa kwesampuli
Ilungele igazi kunye nezicubu ezahlukeneyo zeehagu ezikrokrelwayo ezigulayo: i-spleen, i-lymph node kunye nezicubu zezintso.
Iisampuli zegazi
Thatha i-200 μ L isampuli yegazi, i-5000g centrifugal 5 min, thatha i-supernatant ukuze ihlolwe.
Iisampuli zezicubu
Emva kokuba iisampulu zethishu ziphantsi ngokupheleleyo, inani elifanelekileyo lesaline eqhelekileyo okanye i-PBS yongezwa, kwaye i-supernatant yayiyi-centrifuged ukuze ivavanywe.
2. Utsalo oluzenzekelayo
I-Hangzhou Bigfish Bio-tech Co., Ltd. I-BFEX-32 i-extractor ye-nucleic acid ezenzekelayo inokugqiba ukutsalwa kweesampuli ze-32 kwimizuzu engama-30, enokuhlangabezana neemfuno zakho zokukhupha inani elikhulu leesampuli. Yonke le nkqubo ayifuni nawuphi na omnye umsebenzi, igcina ixesha kwaye inciphisa impazamo yokusebenza ngesandla, kwaye iphucula impumelelo yomsebenzi kangangoko kunokwenzeka.
3. Ukuhlanjululwa okuphezulu kwe-nucleic acid
I-MagPure Nucleic acid extraction kit, kunye ne-BFEX-32, imveliso ihambelana ngokupheleleyo ne-PCR & ne-QPCR yokufunyanwa.
4. Uvavanyo lwekhompyutha kunye nohlalutyo
Ngokwemeko yokwenyani yelebhu, umsebenzisi ufumana iskimu sokubona umgangatho okanye ubungakanani.
I-Hangzhou Bigfish Bio-tech Co., Ltd. ibonelela ngezixhobo zokubona umgangatho (Ubungakanani) be-African classical swine fever (ACFV) kunye neFC-96G (BFQP-16/48), ekwazi ukubona i-ACFV ngokubanzi, ngovelwano nangokuthembeka.
Umxholo ongaphezulu, nceda ubeke ingqalelo kwi-akhawunti esemthethweni ye-WeChat ye-Hangzhou Bigfish Bio-tech Co., Ltd.
Ixesha lokuposa: May-23-2021
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