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Inkqubela enxulumeneyo
Ngokutsho kweOfisi yoLwazi yoMphathiswa wezoLimo neendawo zasemaphandleni, ngo-Agasti 2018, isifo seengulube sase-Afrika sabakho kwiSithili saseNew Shenbei, kwiSixeko saseShenyang, kwiPhondo laseLiaoning, esisifo seengulube sokuqala sase-Afrika eTshayina. Ukusukela nge-14 kaJanuwari 2019, isifo seengulube sase-Afrika sabakho kumaphondo angaphezu kwama-20 eTshayina, sabulala iingulube ezingama-916000, nto leyo eyabangela inkxalabo kuluntu.
Umkhuhlane weengulube waseAfrika (ASF)
I-ASF (i-African Swine fever) sisifo esosulelayo esibangelwa yintsholongwane ye-African swine fever (ACFV) esosulela iihagu zasekhaya kunye neehagu zasendle (i-African wild boar, i-European wild boar, njl.njl.). I-World Organisation for Animal Health (OIE) iyidwelise njengesifo sezilwanyana esichazwe ngokusemthethweni, esikwaluhlobo lobhubhane lwezilwanyana olujoliswe ekuthinteleni iTshayina.
Iimpawu zesifo
Iimpawu zeklinikhi ngumkhuhlane (ukuya kuthi ga kwi-40 ~ 42 ℃), i-tachycardia, ukuphelelwa ngamandla, ukukhwehlela okuncinci, ukukhupha ulwelo olufana ne-serous okanye i-mucinous purulent emehlweni nasempumlweni, i-skin cyanosis, ukopha okucacileyo kwezintso, i-lymph node kunye ne-gastrointestinal mucosa. Iimpawu zeklinikhi ze-African classical swine fever ziyafana nezo ze-classical swine fever, enokufunyanwa kuphela ngokujonga elebhu.
Isisombululo somkhuhlane weengulube waseAfrika oqhelekileyo
1. Ukucubungula isampuli
Ifanelekile kwigazi kunye nezicubu ezahlukeneyo zeehagu ezirhanelwa ukuba zigula: i-spleen, i-lymph node kunye nezicubu zezintso.
Iisampuli zegazi
Thatha isampuli yegazi engama-200 μ L, i-5000g centrifugal imizuzu emi-5, thatha i-supernatant ukuze ihlolwe.
Iisampuli zezicubu
Emva kokuba iisampulu zezicubu zicoliwe ngokupheleleyo, kongezwa isixa esifanelekileyo se-saline eqhelekileyo okanye i-PBS, kwaye i-supernatant yafakwa kwi-centrifuge ukuze ivavanywe.
2. Ukukhupha ngokuzenzekelayo
I-Hangzhou Bigfish Bio-tech Co., Ltd. I-BFEX-32 automatic nucleic acid extractor ingagqiba ukukhupha iisampuli ezingama-32 kwimizuzu engama-30, nto leyo enokuhlangabezana neemfuno zakho zokukhupha inani elikhulu leesampuli. Yonke le nkqubo ayidingi naluphi na olunye usebenzi, igcina ixesha kwaye inciphisa impazamo yokusebenza ngesandla, kwaye iphucula ukusebenza kakuhle kangangoko kunokwenzeka.
3. Ukuhlanjululwa okuphezulu kwe-nucleic acid
Ikhithi yokukhupha i-MagPure Nucleic acid, ene-BFEX-32, imveliso ihambelana ngokupheleleyo nokufunyanwa kwe-PCR kunye ne-QPCR.
4. Uvavanyo lwekhompyutha kunye nohlalutyo
Ngokwemeko yokwenyani yelebhu, umsebenzisi usebenzisa inkqubo yokuchonga esemgangathweni okanye eyobuninzi.
I-Hangzhou Bigfish Bio-tech Co., Ltd. ibonelela ngekhithi yokufumanisa esemgangathweni (eyiQuantitative) ye-African classical swine fever (ACFV) ene-FC-96G (BFQP-16/48), enokuyibona i-ACFV ngokubanzi, ngobunono nangokuthembekileyo.
Umxholo ongakumbi, nceda uqwalasele iakhawunti esemthethweni yeWeChat yeHangzhou Bigfish Bio-tech Co., Ltd.
Ixesha lokuthumela: Meyi-23-2021
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