Uwukhetha njani umjikelo oshushu ofanelekileyo kwiimfuno zakho zophando

Iibhayisekile ezishushuzizixhobo eziyimfuneko xa kufikwa kwibhayoloji yemolekyuli kunye nophando lwemfuza. Esi sixhobo saziwa ngokuba yiPCR (polymerase chain reaction), esi sixhobo siyimfuneko ekwandiseni iDNA, siyenze ilitye lembombo leendlela ezahlukeneyo zokusebenza eziquka i-cloning, ulandelelwano kunye nohlalutyo lwe-gene expression. Nangona kunjalo, zininzi iinketho kwimarike kangangokuba ukukhetha umjikelo oshushu ofanelekileyo kwiimfuno zakho zophando kunokuba ngumsebenzi onzima. Nazi ezinye izinto ezibalulekileyo ekufuneka uziqwalasele xa usenza ukhetho lwakho.

1. Qonda iimfuno zakho zophando

Phambi kokuba ungene kwiinkcukacha zeebhayisekile ezishushu ezahlukeneyo, kubalulekile ukuvavanya iimfuno zakho zophando. Qwalasela uhlobo lovavanyo oza kulwenza. Ngaba usebenzisa i-PCR eqhelekileyo, i-PCR yobungakanani (qPCR), okanye i-high-throughput application? Ngasinye kwezi zicelo zinokufuna iimpawu ezahlukeneyo kunye nesakhono se-thermal cycler.

2. Uluhlu lobushushu kunye nokufana

Uluhlu lweqondo lokushisa lomjikelo we-thermal luyinto ebalulekileyo. Iiprothokholi ezininzi ze-PCR zifuna inyathelo le-denaturation malunga ne-94-98°C, inyathelo lokuthomalalisa kuma-50-65°C, kunye nenyathelo lokwandisa kuma-72°C. Qinisekisa ukuba umjikelo we-thermal owukhethayo unokuphatha la maqondo obushushu kwaye ubushushu busasazwa ngokulinganayo kuyo yonke imodyuli. Ukushisa okusemgangathweni okufanayo kunokuchaphazela uphando lwakho ngokubangela iziphumo ezingahambelaniyo.

3. Ifomathi yebhloko kunye nomthamo

Iibhayisekile ze-Thermal ziza kwiifomathi ezahlukeneyo zemodyuli, kubandakanywa iipleyiti zequla ezingama-96, iipleyiti zequla ezingama-384, kunye namacwecwe angama-1536. Ukukhetha ifomathi yebhloko kufuneka ihambelane neemfuno zakho zokuphuma. Ukuba wenza imifuniselo ephezulu, unokufuna ifomathi yebhloko enkulu. Ngokwahlukileyo, kwiimvavanyo zomgangatho omncinci, ipleyiti yequla engama-96 inokwanela. Ukongeza, jonga ukuba ngaba ufuna iimodyuli ezinokutshintshana kwiifomathi ezahlukeneyo, njengoko oku kunokonyusa ukuguquguquka kophando lwakho.

4. Isantya kunye nokusebenza kakuhle

Kwimeko yanamhlanje yophando olukhawulezayo, ixesha lingundoqo. Jonga ibhayisekile ye-thermal enokufudumeza ngokukhawuleza kunye namandla okupholisa. Ezinye iimodeli eziphambili zinokugqiba umjikelo wePCR ngaphantsi kwemizuzu engama-30, ikhawulezisa kakhulu ukuhamba kwakho komsebenzi. Ukongeza, iimpawu ezinje ngemowudi ekhawulezayo okanye ubushushu obukhawulezayo bonyusa ukusebenza kakuhle, kukuvumela ukuba uqhube iisampuli ezininzi ngexesha elincinci.

5. Ujongano lomsebenzisi kunye neSoftware

Ujongano olusebenziseka lula luyimfuneko ekusebenzeni ngokufanelekileyo. Jonga umjikelo we-thermal nge-intuitive touch screen, iinketho zokucwangcisa ezilula, kunye neeprothokholi ezicwangcisiweyo. Iimodeli ezikwinqanaba eliphezulu zinokuza nesoftware evumela ukubekwa esweni kwexesha lokwenyani kunye nohlalutyo lwedatha, oluluncedo kakhulu kwizicelo ze-qPCR. Qinisekisa ukuba isoftware iyahambelana neenkqubo zakho ezikhoyo kwaye inokusingatha imveliso yedatha oyifunayo.

6. Iingqwalasela zoHlahlo-lwabiwo-mali

Iibhayisekile ezishushu zohluka kakhulu kwixabiso, ngoko ke kubalulekile ukuba ube nohlahlo lwabiwo-mali ngaphambi kokuba uqale ukuyithenga. Ngelixa kunokuhenda ukuhamba neyona ndlela inexabiso eliphantsi, qwalasela ixabiso lexesha elide lokutyala imali kumatshini okumgangatho ophezulu ohlangabezana neemfuno zakho zophando. Ungaqwalaseli kuphela ixabiso lokuqala lokuthenga, kodwa kunye neendleko zokusetyenziswa, ukugcinwa, kunye nokuphuculwa okunokwenzeka.

7. Inkxaso yoMvelisi kunye neWaranti

Ekugqibeleni, qwalasela inqanaba lenkxaso kunye newaranti enikezelwa ngumenzi. Umjikelo othembekileyo we-thermal kufuneka unikeze iwaranti ebanzi kwaye ube nenkxaso yabathengi malunga nokulungisa iingxaki kunye nokugcinwa. Oku kongela ixesha kunye nezixhobo ekuhambeni kwexesha.

ukuququmbela

Ukukhetha okulungileyoumjikelo we-thermalkuba iimfuno zakho zophando sisigqibo esibalulekileyo esinokuchaphazela impumelelo yovavanyo lwakho. Ngokuqwalasela ngononophelo iimfuno zakho ezithile, uluhlu lobushushu, ifomathi yemodyuli, isantya, ujongano lomsebenzisi, uhlahlo lwabiwo-mali, kunye nenkxaso yomenzi, unokwenza ukhetho olunolwazi oluya kukhulisa amandla akho ophando kwaye ufumane iziphumo ezithembeke ngakumbi. Ixesha lokutyala kule nkqubo yokhetho ekugqibeleni liya kuhlawula kumgangatho kunye nokusebenza kakuhle komsebenzi wakho wesayensi.