Ungawukhetha njani umjikelo ofanelekileyo we-thermal kwimfuno yakho yophando

Abajikelezi bomzimbaNgaba izixhobo ezingathandekiyo xa kuziwa kwi-biology yemolekyuli kunye nophando lwemfuza. Kwakhona eyaziwa ngokuba yi-PCR (i-polymerase chain), esi sixhobo sibalulekile ukuze senze iDNA, esenza ukuba ibe lilitye lesiseko sezicelo ezahlukeneyo kubandakanya ukubetha, ukulandelelana kunye nokugcwala kunye nokwahlula-hlula kunye ne-sultening tulcon. Nangona kunjalo, zininzi iindlela zokhetho kwimarike enyula umjikelezo ofanelekileyo we-thermal ukuze iimfuno zakho zophando zinokuba ngumsebenzi otyhafisayo. Nazi ezinye izinto eziphambili ekufuneka ziqwalaselwe xa usenza ukhetho lwakho.

1. Qonda iimfuno zakho zophando

Ngaphambi kokuntywila kwinkcazo yemijikelezo yomlomo ohlukeneyo, kubalulekile ukuba uvavanye iimfuno zakho zophando. Qwalasela uhlobo lovavanyo oya kuqhuba. Ngaba usebenzisa i-PCR ye-PCR, ubungakanani be-PCR (i-QPRCR), okanye usetyenziso olunegalelo eliphezulu? Isicelo ngasinye sinokufuna izinto ezahlukeneyo kunye nezakhono zomjikelezo we-thermal.

2. Uluhlu lobushushu kunye nokufana

Uluhlu lobushushu lomjikelezo we-thermal yinto ebalulekileyo. Uninzi lweeprotocols ze-PCR zifuna inyathelo lezinto ezimalunga ne-94-98 ° C, inyathelo eliyikisayo kwi-50-65 ° C, kunye nenyathelo elongeziweyo kwi-72 ° C. Qinisekisa ukuba umjikelezo we-thermal oyikhethayo unokuphatha amaqondo obushushu kwaye amaqondo obushushu asasazwa ngokuhlwa kuyo yonke imodyuli. Ubushushu obungalunganga bungachaphazela uphando lwakho ngokubangela iziphumo ezingahambelaniyo.

3. Ifomathi yebhloksi kunye nomthamo

Abajikelezi be-thermal beza ngeendlela ezahlukeneyo zemodyuli, kubandakanya iipleyiti ezingama-384, iipleyiti ezingama-384, kunye ne-1536-iipleyiti. Ukhetho lwefomathi yebhloks kufuneka ihambelane neemfuno zakho zokufaka. Ukuba wenza uvavanyo lokufaka eliphezulu, unokufuna ifomathi yebhloko enkulu. Kwelinye icala, ukuvavanywa okuncinci, i-96-pile kakuhle inganele. Ukongeza, cinga ukuba ngaba ufuna iimodyuli ezingenankathane kwiifomathi ezahlukeneyo, kuba oku kungayonyusa uqoqosho lophando lwakho.

4. Isantya kunye nokusebenza kakuhle

Kwimeko yophando yanamhlanje ekhawulezileyo yophando, ixesha liyinto ephambili. Jonga umjikelezo we-thermal ngokufudumeza ngokukhawuleza kunye nokupholisa amandla. Ezinye iimodeli eziphambili zinokugqiba umjikelo we-PCR ongaphantsi kwemizuzu engama-30, ukhawulezisa kakhulu ukubetha kwakho. Ukongeza, iimpawu ezinje ngemodi ekhawulezayo okanye amaxabiso okutshutshisa ngokukhawuleza akhulisa ukusebenza kakuhle, akuvumele ukuba uqhubekele iisampulu ezininzi ngexesha elincinci.

5. Imbonakalo yabasebenzisi kunye nesoftware

Ujongano lomsebenzisi lubalulekile ekusebenzeni kakuhle. Jonga umjikelo we-thermal ngesikrini sokuchukumisa esingekaloni, iindlela ezilula zenkqubo, kunye neeprotokholi ezenziweyo. Iimodeli eziphambili zinokuza nesoftware evumela ukubeka iliso kwixesha lokwenyani kunye nohlalutyo lwedatha, oluyingenelo ikakhulu kwizicelo ze-QPCR. Qiniseka ukuba isoftware iyahambelana neenkqubo zakho ezikhoyo kwaye ungasingatha igalelo ledatha oyifunayo.

6. Iinkangeleko yoHlahlo-lwabiwo mali

Abajikelezi bomzimba bahluka kakhulu ngexabiso, ngoko kubaluleke kakhulu ukuba nohlahlo-lwabiwo mali ngaphambi kokuba uqale ukuthenga enye. Ngelixa kunokuhendela ukuhamba nendlela yexabiso eliphantsi, cinga ngexabiso lexesha elide lokutyala kumatshini omgangatho ophezulu ohlangabezana neemfuno zakho zophando. Khawuqwalasele kuphela ixabiso lokuthenga lokuqala, kodwa neendleko zokusetyenziswa, ukugcinwa, kunye nokuphuculwa okunokubakho.

7. Inkxaso yomvelisi kunye newaranti

Okokugqibela, cinga ngenqanaba lenkxaso kunye newaranti ebonelelwe ngumenzi. Umjikelezo we-therm othembekileyo kufuneka unike iwaranti ebanzi kwaye ube nenkxaso yabathengi yokulungisa ingxaki kunye nokugcinwa. Oku kuyigcina ixesha kunye nezixhobo ekuhambeni kwexesha.

ukuququmbela

Ukukhetha ilungeloumjikelezo we-thermalKuba iimfuno zakho zophando sisigqibo esibalulekileyo esinokuba nefuthe kwimpumelelo yovavanyo lwakho. Ngokujonga ngononophelo kwiimfuno zakho ezithile, uluhlu lobushushu, ifomathi yemodyuli, isantya, uhlahlo-lwabiwo mali, nohlahlo-lwabiwo mali, kunye nokukhetha umvelisi, unokwenza iziphumo ezinolwazi eziya kuphucula iziphumo zakho. Ixesha lokutyalomali kule nkqubo yokhetho liya kuthi ekugqibeleni lihlawule kumgangatho kunye nokusebenza kakuhle komsebenzi wakho wesayensi.