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I-reagent entsha yokubona isifo sehagu esiqandisiweyo esivela kwiBig Fish iqalisiwe. Ngokungafaniyo nee-reagents zemveli zokubhaqa ulwelo ezifuna ukulungiswa ngesandla kweenkqubo zokusabela, le reagent isebenzisa ifom ye-microsphere exutywe ngokupheleleyo ne-freeze-dryed, enokugcinwa kubushushu begumbi ngaphandle kokuchaphazela ukusebenza kwe-reagent. Ngexesha lokufunyanwa, yi-nucleic acid ekhutshiweyo kuphela ekufuneka yongezwe ngokuvula isiciko. Emva kokuba i-reagent inyibilikisiwe ngokupheleleyo, inokuvavanywa kumatshini. Idibene nee-reagents ezikhupha i-nucleic acid ezizenzekelayo kunye neemveliso zezixhobo ezivela kwiBig Fish, iBig Fish iqalise ngokusesikweni isisombululo sokubhaqa ngokukhawuleza semizuzu engama-40 sezifo zehagu. Ngeeprojekthi ezintandathu ezinkulu zokubhaqa eziquka iBlue Ear, iPseudorabies, iSwine Fever, iCircovirus, iNon Circovirus, kunye nePorcine Influenza, kuthatha malunga nemizuzu engama-40 kuphela ukugqiba yonke inkqubo yokubhaqa ubungakanani be-PCR fluorescence ukusuka ekucwangcisweni kwesampuli ukuya kwiziphumo zokubhaqwa.
Inkqubo yeSisombululo
1. Ukukhupha ngokufanelekileyo - iisampulu ezininzi zingacutshungulwa kwimizuzu eli-10
Ngokusebenzisa i-Big Fish universal nucleic acid extraction and purification reagent edityaniswe nesixhobo sokukhupha kunye nokucoca i-nucleic acid esizenzekelayo ngokupheleleyo, ukukhupha i-nucleic acid kungenziwa kwiisampuli ezahlukeneyo (kuquka igazi elipheleleyo, i-serum, i-plasma, ii-environmental swabs, ii-oral swabs, ii-fecal swabs, njl.njl.) kwimizuzu eli-10 ngaphandle kwesidingo sonyango oluyinkimbinkimbi lwangaphambi kokuba isampuli inyangwe. Emva kokulayisha isampuli, ingakhutshwa kumatshini.
2. Ukwandiswa ngokukhawuleza -30 min ngokukhawuleza fluorescence quantification
Ngokusebenzisa iBig Fish BFOP-1650 Fluorescence Quantitative PCR Analyzer, i-wild mode ye-Big Fish freeze dry detection reagent ingasetyenziswa. Ukudibanisa ii-freeze-drying reagents kunye nenkqubo yokufumanisa ngokukhawuleza yemizuzu engama-30 kunokufezekisa uvavanyo oluvulekileyo kunye novavanyo lwangaphakathi.
3. Uhlalutyo olukrelekrele - ukusebenza kwesitshixo, uhlalutyo oluzenzekelayo
I-Big Fish BFOP-1650 fluorescence quantitative PCR analyzer ayifuni useto lwenkqubo oluntsonkothileyo. Cofa u-"Qala" kuzo zonke izinto zokufumanisa ukuze uqalise ukufumanisa. Emva kokuba i-amplization igqityiwe, yenza ngokuzenzekelayo ugwebo oluhle nolubi ngaphandle kohlalutyo lwedatha ngesandla.
Ixesha leposi: Juni-21-2025
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