Iindaba - Vula isiciko kwaye ukhangele - Intlanzi Enkulu I-40mins isifo sehagu isisombululo sokufumanisa ngokukhawuleza

Vula isiciko kwaye ukhangele - Iintlanzi ezinkulu ze-40mins isifo sehagu ngokukhawuleza isisombululo

Isixhobo esitsha sokufumanisa isifo sehagu sinomkhenkce kwintlanzi enkulu iye yasungulwa. Ngokungafaniyo neereagents zokubona ulwelo zemveli ezifuna ukulungiswa ngesandla kweenkqubo zokusabela, le reagent ithatha i-microsphere exutywe ngokupheleleyo ngaphambili exutywe ngumkhenkce, enokugcinwa kwiqondo lobushushu begumbi ngaphandle kokuchaphazela ukusebenza kwe-reagent. Ngethuba lokufumanisa, kuphela i-nucleic acid ekhutshweyo kufuneka yongezwe ngokuvula isiciko. Emva kokuba i-reagent ichithwe ngokupheleleyo, inokuvavanywa kumatshini. Idityaniswe ne-automated nucleic acid extraction reagents kunye neemveliso zesixhobo ezivela kwi-Big Fish, i-Big Fish iye yasungula ngokusemthethweni isisombululo se-40 esikhawulezayo sokubona izifo zehagu. Ngeeprojekthi ezintandathu eziphambili zokubhaqa eziquka i-Blue Ear, i-Pseudorabies, i-Swine Fever, i-Circovirus, i-Non Circovirus, kunye ne-Porcine Influenza, kuthatha kuphela malunga nemizuzu engama-40 ukugqiba yonke inkqubo ye-PCR fluorescence yokufumanisa ubungakanani ukusuka kwisampuli yesampula ukuya kwiziphumo zokubona.

Inkqubo yesisombululo

1. Ukukhutshwa okusebenzayo - iisampuli ezininzi zinokucutshungulwa kwimizuzu eyi-10
Ngokusebenzisa i-Big Fish universal nucleic acid extraction kunye ne-reagent yokucoca idityaniswe ne-automatic nucleic acid extraction kunye nesixhobo sokucoca, i-nucleic acid extraction inokwenziwa kwiisampuli ezahlukeneyo (kubandakanywa negazi elipheleleyo, i-serum, i-plasma, i-swabs yokusingqongileyo, i-swabs yomlomo, i-facal swabs, njl. Emva kokulayisha isampuli, inokukhutshwa kumatshini.

2. Ukukhulisa ngokukhawuleza -30 min ubungakanani be-fluorescence obukhawulezayo
Ngokusebenzisa i-Big Fish BFOP-1650 Fluorescence Quantitative PCR Analyzer, indlela yasendle ye-Big Fish freeze i-reagent yokufumanisa eyomileyo inokusebenza. Udibaniso lwee-reagents zokumisa umkhenkce kunye ne-30 imizuzu yenkqubo yokufumanisa ngokukhawuleza inokufikelela ngokwenene kwisiciko esivulekileyo kunye novavanyo lwesayithi.

3. Uhlalutyo oluhlakaniphile - umsebenzi obalulekileyo, uhlalutyo oluzenzekelayo
I-Big Fish BFOP-1650 fluorescence quantitative PCR analyzer ayifuni izicwangciso zeprogram ezinzima. Nqakraza "Qala" kuzo zonke izinto zokubona ukuqalisa ubhaqo. Emva kokuba i-amplification igqityiwe, yenza ngokuzenzekelayo isigwebo esilungileyo nesibi ngaphandle kokuhlalutya idatha ngesandla.

Ixesha lokuposa: Jun-21-2025