Ukwenza i-PCR yokuSebenzisa i-PCR isebenzisa umjikelo we-therrm ophambili

I-Polymerase Chain Rein Impendulo (i-PCR) yindlela esisiseko kwi-molecular biology kwaye isetyenziselwa kakhulu ukukhulisa ukulandelelana kwe-DNA. Ukusebenza kunye nokuchaneka kwe-PCR kuchaphazeleka kakhulu ngumjikelezo we-thermal osetyenziswe kwinkqubo. Abajikelezile bomzimba ophambili badlala indima ebalulekileyo ekupheliseni i-PCR YAPH, ukubonelela ngolawulo lobushushu obufanelekileyo, ubushushu obukhawulezileyo kunye namaxabiso okupholisa, kunye nenkqubo yokuphucula amandla.

Enye yeempawu eziphambili ze-AddAbajikelezi bomzimbaUlawulo lobushushu oluchanekileyo. Ukugcina amaqondo obushushu athile okuphembelela, ukungcungcuthekisa, kunye namanyathelo ongezelelweyo aluleke kwi-prc ephumeleleyo yePCR. Umjikelezo we-thermal owuqhubela phambili uqinisekisa iyunifomu kunye nechanekileyo ekunikezelweni kwamaqondo obushushu kuzo zonke iikopi, ukunciphisa umahluko ekusebenzeni kakuhle kunye nokunciphisa ukubakho kokuzonwabisa okungacacanga.

Ukushiyeka ngokukhawuleza kunye namazinga okupholisa yenye into ebalulekileyo kwimijikelezo yobume bomzimba. Ezi zixhobo zixhotyiswe ngetekhnoloji esekwe kwi-petlier enokutshintshela ngokukhawuleza phakathi kwamanyathelo ohlukeneyo obushushu. Oku ngebhayisikile ekhawulezileyo engagciniweyo kuphela kodwa kunciphisa umngcipheko wokuqulunqwa kwe-primer-dimer kunye ne-pumer ye-pimer, ngaloo ndlela ingenzi njalo, ngaloo ndlela inyusa ubunyani be-prc kunye nokusebenza kakuhle.

Ukongeza, abajikelezayo obuphambili be-thermas ophambili bonyusa amandla okuqhubela phambili, ukuvumela abasebenzisi ukuba bacwangcise iiprothokholi zePCR kwiimfuno zabo zovavanyo. Ezi zixhobo zinikezela ngokuguquguqukayo ukuseta i-PRCR PRCR, i-PCR, kunye nezinye iiprothokholi ezizodwa, ukunika amandla okwenza izinto ze-PCR zeeseti zeprinta ezahlukeneyo kunye neetemplate. Ukongeza, abanye abahamba nge-thermatics baxhotyiswa ngeSoftware yeSoftware eyenza lula iProtpool kunye nohlalutyo lwedatha, ngaloo ndlela kuphucule ukusebenza kakuhle.

Ukongeza kwezi mpawu, abanye abahamba nge-thermas banikezela ngetekhnoloji yokufumana itekhnoloji yokuthintela ukuvumelana nokuhamba ngebhayisikile, ukuqinisekisa ukulahleka kwesampulu. Abanye banokubandakanya umsebenzi we-gradient onokwandisa amaqondo obushushu aqatha kwiisampulu ezininzi ngaxeshanye, ngakumbi ukuphucula ukusebenza kakuhle kwe-PCR kunye nokuthembeka.

Ukubaluleka kokusebenzisa umjikelo we-thermal we-thermas ophezulu ukwenza i-PCR yokusebenza akunakuba ngaphezulu. Ezi zixhobo zingangenzi lula inkqubo ye-PCR kuphela kodwa zikwanceda ekuphuculeni ukuzala kunye nokuchaneka kweziphumo zovavanyo. Ngokubonelela ngolawulo lobushushu obufanelekileyo, ukuhamba ngebhayisikile ngokukhawuleza, kunye nokuhamba phambili kwenkqubo yenkqubo yenkqubo, abahamba ngemigca ye-thermation bavumela ukuba abaphambukeli bezinto ezahlukeneyo, kubandakanya nohlalutyo lwesimo se-genobing, elecling, kunye nokudakumba.

Ukuqukumbela, phambiliAbajikelezi bomzimbaDlala indima ephambili ekupheliseni i-PCR yokuSebenzisa. Ukulawulwa kweqondo lobushushu elichanekileyo, ukufudumeza ngokukhawuleza kunye namazinga okupholisa, kunye nezakhono zoMgaqo-nkqubo weNkqubo Abaphandi banokuxhamla kakhulu ngokusebenzisa umjikelo we-thermal we-thermas ephambili kwi-moleculager yebhayoloji, ekugqibeleni ikhokelela ekufumaneni okuthembekileyo kwesayensi kunye nokuqonda.