I-PCR DenawshooOOOCSOOTION

I-Polymerase Chain Reances (PCR) zizinto ezibalulekileyo kwizixhobo kwi-molecular, ivumela abaphandi ukuba bangenise i-DNA kwizicelo zophando lwezonyango. Nangona kunjalo, njengaso nasiphi na isixhobo esinzima, uhlalutyo lwe-PCR lunokudibana neengxaki ezichaphazela ukusebenza kwalo. Eli nqaku lijongana neminye imibuzo eqhelekileyo malungaI-PCR SultizerUkucombulula ingxaki kwaye unike izisombululo ezisebenzayo kwiingxaki eziqhelekileyo.

1. Kutheni le nto indlela yam ye-PRR ingakhuli?

Enye yezona ngxaki ziqhelekileyo zijamelene nabasebenzisi kukungakwazi kwe-PCR yokuphendula ukuze inyuse iDNA ekujoliswe kuyo. Oku kunokubangelwa kwizinto ezininzi:

Uyilo lweprinta engalunganga: Qiniseka ukuba i-primers yakho ikhethekileyo kulandelelwano lwethagethi kwaye inobushushu obufanelekileyo (TM). Sebenzisa izixhobo zesoftware yoyilo lweprinta ukunqanda ukubopha.

I-DNA yaneleyo i-template ye-template: Qinisekisa ukuba usebenzisa inani elaneleyo le-DNA yetemplate. Kancinci kakhulu kuya kubangela ukuba ubuthathaka okanye ungabinakho.

I-inhibitors kwiSampulu: I-Companants kwisampulu inokuthintela i-PCR yokuphendula. Qwalasela ukucoca i-DNA yakho okanye usebenzisa indlela yokukhupha eyahlukileyo.

Isisombululo: Jonga uyilo lwakho lwe-primer, nyusa itemplate yoxinzelelo, kwaye uqiniseke ukuba isampuli yakho ayinabaxhasi.

2. Kutheni le nto i-PCR yam ye-PCR?

Ukuba ubungakanani bemveliso yakho ye-PCR ayilindelanga, inokubonisa ingxaki ngeemeko zokuphendula okanye izithako ezisetyenzisiweyo.

I-non-amunition ye-on-comphar: Oku kunokwenzeka ukuba i-primer ibophelela kwindawo engafakwanga. Jonga ubuso beeprims usebenzisa isixhobo esifana nokuqhuma.

Iqondo lobushushu elingachanekanga: Ukuba amaqondo obushushu angeyonyani aphantsi kakhulu, anokubangelwa kukhethekileyo. Ukuphucula amaqondo obushushu angenisayo yi-GRCR.

Isisombululo: Qinisekisa ubuchule bokuqala kwaye uqinisekise ubushushu obubonakalayo ukuphucula ukuchaneka kweemveliso zePCR.

I-3. I-PRR Surchazer yam ibonisa umyalezo wemposiso. Ndingenza ntoni?

Imiyalezo yempazamo kwi-PCR Denagemer inokubakho, kodwa zihlala zinika imikhondo kwiingxaki ezinokubakho.

Imiba yokulinganisa: qinisekisa ukuba i-PCR Denawer ilungelelaniswe ngokuchanekileyo. Ukugcinwa rhoqo kunye neetsheki zokulinganisa kubalulekile ukufumana iziphumo ezichanekileyo.

IQela leSoftware: Ngamanye amaxesha, i-bugs yesoftware inokubangela iingxaki. Qala kwakhona ikhompyuter yakho kwaye ujonge uhlaziyo lwesoftware.

Isisombululo: Jonga kwincwadana yemisebenzisi kwikhowudi ethile yempazamo kwaye ulandele amanyathelo acetyiswayo. Ukulungiswa rhoqo kungathintela iingxaki ezininzi.

4. Kutheni le nto i-PRR yam ye-PRR ibangelwa kungangqinelani?

Iziphumo zePCR ezingahambelaniyo zinokukhathaza izizathu ezininzi:

Umgangatho wokuqalisa: Qinisekisa ukuba zonke ii-regents, kubandakanya i-enzyme, i-buffers, kunye nee-DNTE, zintsha kwaye zikumgangatho ophezulu. I-Redegents ephelelwe lixesha okanye engcolisekileyo inokubangela ukungafani.

I-thermation yokuhambahamba: Useto lobushushu olungahambelani lunokuchaphazela inkqubo ye-PCR. Jonga rhoqo ukulinganisa komjikelezo we-thermal.

Isisombululo: Sebenzisa i-regents yomgangatho ophezulu kwaye ucoce umjikelo wakho we-thermal rhoqo ukuqinisekisa iziphumo ezingaguqukiyo.

5. Uphucula njani ukusebenza kwe-pRR?

Ukuphuculwa kokusebenza kakuhle kokuthatha inxaxheba kwePRR kunokukhokelela kwizivuno eziphezulu kunye neziphumo ezinokuthenjwa.

Yenza uqhuba iimeko zokuphendula: Uvavanyo usebenzisa ugxininiso olwahlukileyo lwe-primes, itemplate ye-DNA kunye neMGCL2. Impendulo nganye ye-PCR inokufuna iimeko ezizodwa zomsebenzi ofanelekileyo.

Sebenzisa i-enzymes ephezulu ye-Enzymes: Ukuba ukuchaneka kubalulekile, kuqwalasela ukusebenzisa i-DNA Polymerase ye-DNA ye-DNA ukunciphisa iimpazamo ngexesha lokukhula.

Isisombululo: Yenza uvavanyo loKhetho lokufumana iimeko ezilungileyo zokuseta okukhethekileyo.

Isishwankathelo

Ukucombulula i-AI-PCR SultizerInokuba ngumsebenzi otyhafisayo, kodwa ukuqondeka iingxaki eziqhelekileyo kunye nezisombululo zabo zingaphucula kakhulu amava akho prc. Ngokusombulula ezi ngxaki ziqhelekileyo, abaphandi banokuphucula iziphumo ze-PCR kwaye baqinisekise iziphumo ezinokuthenjwa kwizicelo ze-molecular biology. Ukulungiswa rhoqo, ukukhetha ngononophelo kweendawo zokuhlala, kunye nokuphuculwa kweemeko zokuphendula ziindlela zokuphumelela kuhlalutyo lwe-PCR.