Ukusebenza kwezine ze-acid i-acid acid asflis ukukhangela i-SARS-cov-2 e-Ethiopia

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Ukususela kwisifo se-CODOVIRUS ka-2019 (Covid-19) Ukuqhambuka, iimvavanyo ezininzi ze-nucleid ze-acid acid (iNaits) ziye zaphuhliswa kwihlabathi liphela kwaye zaba nezithambisi ezisemgangathweni. Nangona iimvavanyo ezininzi zaphuhliswa ngokukhawuleza zaza zafakwa kwiimvavanyo zokuxilongwa kwelebhu, ukwenziwa kwezi vavanyo akuzange kuvavanywe kwiinkqubo ezahlukeneyo. Ke ngoko, olu phononongo lujolise ekuvavanyweni ukwenziwa kwentsebenzo ye-Abbott Sars-Cov-2, i-daan, i-BGI, kunye nePotech Biotech Isifundo senziwe kwiZiko lezeMpilo lase-Ethiopia) ukusuka nge-1 ukuya kwi-30 kaDisemba 2020. 164 I-164 I-164 I-Nasopharyngezwa le-Nasepharyngezwa i-qiaamp ye-chainikhi ye-QNA MINI Ye-164 yeesampulu, i-59.1% yayiqinisekile kwaye i-40.9% yayingalunganga kwii-CRS. I-Ponstore ye-biotech postity yayiphantsi ngokuthelekiswa kakhulu kwi-CRS (P <0.05). I-Ponstore ye-biotech postity yayiphantsi ngokuthelekiswa kakhulu kwi-CRS (P <0.05). I-положжжжжье были сравнене сравнене сравнене сра сравненю сравненю Iziphumo ze-biotech ze-biotech ze-biotech zaphantsi xa kuthelekiswa ne-CRS (P <0.05).与 I-CRS 相比, i-binred biotech 的阳性率显着较低 (p <0.05).与 I-CRS 相比, i-binred biotech 的阳性率显着较低 (p <0.05). I-у yongenisa i-biotech было сравненьше п I-PRS Imotered biotech yayineziphumo ezimbalwa ezithandekayo xa kuthelekiswa ne-CRS (P <0.05).Isivumelwano sisonke sohlalutyo abane yayingu-96.3-100% xa kuthelekiswa nee-CRS. Ukongeza kwinqanaba le-POSTIIP le-PERNACHCHIUCH CHOOCH, ukwenziwa kwezinto ezine ze-ASSESS sele ziphantse zalingana. Kananjalo, i-biotech ye-biotech [uphando kuphela (ROO)] I-ASOY ifuna ukuqinisekiswa kwayo ngokusetyenziswa kwayo e-Ethiopia. Okokugqibela, uphando olongezelelweyo kufuneka luthathelwe ingqalelo ukuvavanya ukufuduswa kunye namabango omenzi ofanelekileyo.
Ukuvavanywa kwelebhu yinxalenye yombutho wezeMpilo weHlabathi (i-WHO) yesicwangciso sobuchule seSifo se-COROVIrus 201 (Covid-19) ukulungiselela kunye nempendulo (SPRP). Ocebisa ukuba amazwe kufuneka akhe amandla elebhu yokuphucula ukulungiswa, ulawulo olululo lwetyala, ukuhlala nangokuhlala uphendula kwimiceli mngeni yoluntu. Oku kuphakamisa ukuba indima yelebhu iphambili ekubonakaliseni isifo kunye nesifo sobuqhetseba kusulelo olungenasulelayo kunye nokulawula ukusasazeka kwabo.
Ukuchongwa kweCovids-19 ifuna ulwazi lwendalo kunye nonyango, iimpawu zomntu / iimpawu zomntu, kunye nedatha ye-radiagraphic2. Kuba iCovid-19 qaphela yaxelwa eWuhan, China, uninzi lweemvavanyo ze-nucleid ze-acid acid acid (Naats) ziye zaphuhliswa kwihlabathi liphela. I-Realript yexesha lokuTshintshela kwe-Polymerase Tainmer (i-RTT-PCR) isetyenziswe njengeyona ndlela yesiqhelo kunye nendlela emiselweyo yokuphembelwa kwelebhu ye-Syndory ye-Syndrome 2 (i-SARS-2) 3 usulelo. Ukuchongwa kwemolekyuli ye-SARS-2 kuqhelekileyo kusekwe kwi-N (i-Nucloocapsid protein), iRhasi yeVenkile ye-Geerin), kunye ne-genepy (i-genepy gene) ye-genesmerase ye-orf1a / b (b). I-Gene) Ingingqi echongiweyo kwi-chinome yentsholongwane. Bathatyathwa njengeyona mimandla iphambili egciniweyo efumaneka kwi-genomes yentsholongwane yokwamkelwa kwentsholongwane. Phakathi kwezi zemfuza, i-RDRP kunye ne-ENE zinemvakalelo yokufumana uhlalutya, ngelixa i-NE Gen ineemvakalelo eziphantsi zohlalutyo.
Intsebenzo ye-PCR SOSTASS ingahluka ngokuxhomekeka kwimiba eyahlukeneyo enje: Ukuhanjiswa kwee-rements, indlela yokuqasha / indlela yokuqasha, umgangatho woomatshini we-PCR kunye nezinye izixhobo. Ukusukela nge-20 ka-Epreli, ngaphezu kwezixhobo ezahlukeneyo zokuxilonga ezivela kumazwe alithoba afumene ugunyaziso lokusetyenziswa ngongxamiseko E-Ethiopia, ngaphezulu kwe-14 lamaqonga ayi-14 e-PRR esetyenziselwa ukubonwa kwe-SARS-2 kumaziko angama-20 ama-26 kumaziko angama-20, i-ABBO500, i-abbott m2000, i-roche 45000 kunye ne-roche-studio7. Ukongeza, iikhithi ezahlukeneyo ze-PCR zovavanyo lwe-PCR, ezinje ngovavanyo lwe-daan ye-gene, i-abbott sars-2, uvavanyo lwe-biolote, kunye ne-SARS-2 ye-BGE-2 BGI. Nangona i-RTT-PRRE inovelwano kakhulu, abanye abaguli abaneCovid-19 baxela iziphumo ezibi ze-chibonucleclecic (i-RNA) kwiisampuli ze-chibonucleclecic (iRNA) kwiisampuli ze-chibonucleclecic (ezothutho, ukugcinwa kunye nokuvavanywa kwelabhoratri. Iimeko kunye nezenzo zabasebenzi. Ukongeza, isampulu okanye ukulawula ukungagungqi, ukuseta umjikelo (i-CT), kunye nokusebenza kwe-acid ye-acids okanye engasebenziyo / i-SARS-2 RN-2 RN-2 RN-2 RNA inokukhokelela kwimiphumo emihle yeRTR-PCR9. Yiyo loo nto, kucacile ukuba uvavanyo lwe-PCR lunokuchonga abathwali beziqwenga zemfuza, njengoko bengakwazi nokuchonga i-genes yentsholongwane ebonakalayo, ngenxa yoko iimvavanyo zinokuchonga kuphela abaguli hayi abaguli. Ke ngoko, kubalulekile ukuba uvavanye intsebenzo yokuqonda usebenzisa iindlela ezisemgangathweni kwimeko yethu. Nangona uninzi lweNaat Reagentnts lufumaneka kwiZiko lezeMpilo lase-Ethiopia Ke ngoko, olu phononongo lujolise ekuvavanyweni intsebenzo yokuthelekisa iikhithi ezifumanekayo ezifumanekayo zokufunyanwa kwe-SARS-2 nge-2 nge-RTR-2 nge-RTR-pr-prc usebenzisa iisampuli zekliniki.
Bebonke babathathi-nxaxheba abayi-164 abane-Coid-19 ababandakanyiwe kolu phando. Uninzi lweesampuli zazisemaziko onyango (118/164 = I-72%), ngelixa abathathi-nxaxheba abangama-46 (28%) bavela kumaziko angezonyango. Phakathi kwabathathi-nxaxheba azange baphathwe kwiziko, i-15 (9.1%) babenamatyala ngokweekliniki kwaye abangama-31 (18.9%) babenabafowunelwa bamatyala aqinisekisiweyo. Abathathi-nxaxheba abangamashumi asithoba anesithathu (abangama-56.7%) babengamadoda, kwaye intsingiselo (± SD) yeminyaka engama-31.10 (± 11.22) iminyaka.
Kolu phononongo, amanani aqinisekileyo kunye namaxabiso ovavanyo olune kwiCodd-19 ayemiselweyo. Ke, amaxabiso afanelekileyo e-Abbott Sars-2 Assay, i-DEAAN 2019-I-NCOV-2 I-BGECH-2 I-BGECH, i-58.5%, i-55.5% ngokwahlukeneyo. Amanqaku afanelekileyo afanelekileyo kunye nobubi amanqaku enkcazo-cala (i-CRS) yayingama-97 (59.1%) kunye ne-67 (40.9%), ngokulandelelana (iTheyibhile 1). Kolu phononongo, inkcazo ye-CRS yayisekwe kwi "Nawuphi na umgaqo" oqinisekileyo, apho uphume khona kwiziphumo zovavanyo ezine, ezinye izinto ezizeleyo zovavanyo eziphethwe sisiphumo okanye zinengxaki yokwenyani.
Kolu phando, sifumene isivumelwano sepesenti esibi (i-NPA) ye-100% (i-95% (95% ci 94.6-100) kubo bonke uhlalutyo xa kuthelekiswa nee-CRS. Uhlalutyo lwe-biotechnology lwe-biotechnology lubonise i-PPA encinci ye-93.8% (i-95% ye-ci 87.2-97.1) kunye nohlalutyo lwe-gean ngo-2019-ges. Ngokwahlukileyo, isivumelwano esipheleleyo phakathi kwe-SARS-2 ye-BGI-2 BGI kunye ne-biotech biorech 2019-i-NCOV Assay yayingama-98.8% kwaye nge-96.3, ngokwahlukeneyo (iTheyibhile 2).
I-COHEN KAPPA ye-Kappa Conced yeSivumelwano phakathi kwe-CRS kunye ne-ABBOT SARS-2 Iziphumo ze-Abbott-2 i-Assay yayingaguquguquki ngokupheleleyo (k = 1.00). Ngokufanayo, i-Kappa kaCohen ifunyenwe nguDaan Gene 2011 Kolu vavanyo lokuthelekisa, uvavanyo lwe-Chi-Square (i-MCNY TART) ibonisa ukuba iziphumo ze-biotech ye-2019-i-NCOVs Dotech Dotech ihluke kakhulu kwiziphumo ze-CRS (P = 0.031) (iTheyibhile 2).
Njengoko kubonisiwe kwi-Fig.Ipesenti yexabiso eliphantsi le-CT (<20 ct) le-Abbott Sars-2 i-Assay (i-RDRP kunye ne-NCOV) yayiyi-507.3% yexabiso le-gene (i-46-40 ye-CT) yayiyi-3.2%. Ipesenti yexabiso eliphantsi le-CT (<20 ct) le-Abbott Sars-2 i-Assay (i-RDRP kunye ne-NCOV) yayiyi-507.3% yexabiso le-gene (i-46-40 ye-CT) yayiyi-3.2%.Njengoko kubonisiwe kwi-Fig.1, проценьшего значения CT (<20 ct) аначна I-Arbott ye-Abbott гна Orf1a / b аннализа I-Biotech biecki 2019-NCOV покза что что I-CT) сосасавло i-3,2%. 1, Ipesenti yexabiso eliphantsi le-CT (<20 ct) ye-Abbott Sars-2如图 1 所示, Abbott Sars-2 检测 (结合 结合) 的最低 I-CT 基因 Njengoko kubonisiwe kumzobo 1, eyona pesenti yexabiso eliphantsi (<20 ct) yovavanyo lwe-Abbott Sars-Cov-2 I-3.2%. 1, ано I-ра мре 87,6%, а з з1A / b в Ngex Njengoko kubonisiwe kumzobo 1, i-Abbott Sars-2 Assay (edibanisa i-RDRP kunye ne-NEEN) enexabiso le-CT (<20 ct) kwi-Arhente ye-ORANTARY BIOD-Uhlalutyo lwe-NCOV lubonise i-CT ephantsi. Проццнй значтний Ipesenti yamaxabiso (<20 ct) yayingama-50.3%, kunye nepesenti yamaxabiso aphezulu e-CT (36-40 ct) yayiyi-3.2%.I-ABBT SARS-2B uvavanyo lwe-BOV-2B irekhodwe ngokunqongophala kwe-30. Kwelinye icala, kwi-BGI Sars-2 Assay Orf1a / B Gene yayinexabiso eliphezulu le-CT (> 36 ct) ipesenti ibine-4% (FIT. 1). Kwelinye icala, kwi-BGI Sars-2 Assay Orf1a / B Gene yayinexabiso eliphezulu le-CT (> 36 ct) ipesenti ibine-4% (FIT. 1). С proуо Kwelinye icala, kuhlalutyo lwe-BGI Sars-Cov-2 i-Geed1a / B wayenexabiso eliphezulu le-CT (> 36 ct), ipesenti eyayine-4% (Fig. 1).另一方面, 在 BGI SARS-Cov-2 检测中, Orf1a / B 基因具有高 CT 值 (> 36 ct) 的百分比为 4% (¶ 1). Kwelinye icala, kwi-BGI Sars-2, ipesenti ye-Orf1a / B Gene ngexabiso eliphezulu le-CT (> 36 ct) yi-4% (umzobo 1). С с ·уо> пторон Kwelinye icala, kuhlalutyo lwe-BGI sar-2, ipesenti ye-Orf1a / B Geeds ngamaxabiso aphezulu e-CT (> i-36 ct) yayiyi-4% (FT. 1).
Kolu phononongo, sathatha iisampulu ze-164 NasonApharngezwanga. Kuzo zonke iintlobo ze-assay, i-rina bodwang kunye ne-amphation yenziwa kusetyenziswa iindlela neekhiphetha ezicetyiswa ngabavelisi abachaphazelekayo.
Olu phononongo lubonakalise ukuba uvavanyo luka-Arbott lwe-Arbott lwe-SARS-Cov-2 luye lwentsebenzo efanayo ne-CRS, nge-100% elungileyo, nengalunganga, kunye nemvumelwano epheleleyo. IsiVumelwano se-COHEN yi-1.00, sibonisa isivumelwano esipheleleyo kunye ne-CRS. Isifundo esifanayo seDyunivesithi yaseWashington e-US sifumanise ukuba uvavanyo lwe-Arbott lwe-Ars-2 lwalune-93%, ngokuthelekisa i-ASCY-2% ye-CDC. 11. Inkqubo yokuchonga i-Abbott ye-Abbott-2 isekwe ekufumaneni okuhlanganisiweyo kunye ne-RDRP yemfuza, njengoko zombini ezi ntlobo zinobuthathaka, zinciphise iziphoso zobuxoki12. Isifundo eVienna, e-Austria sabonisa ukuba imiqulu yesampulu yesampulu kunye nokufunyanwa kweqhinga elinciphiselweyo kunye nokunyusa umgangatho wokufunyanwa kwempumelelo13. Yiyo loo nto, umdlalo ogqibeleleyo we-Abbott we-ABR-2 ASASY unokunxulunyaniswa nenkqubo yokuchonga iqonga elifumana i-genes edluleyo, ikhuphe inani elikhulu leesampulu (i-0.5 ml), kwaye isebenzisa isixa esikhulu (40 μl).
Iziphumo zethu zikwabonisa ukuba ukufunyanwa kwentsebenzo yovavanyo lwemfuza ye-daan kwakufana ne-CRS. Oku kuyahambelana nesifundo seDyunivesithi yaseAnuhui eHuinan, China, kunye neBango loMvelisi wesivumelwano se-100%. Ngaphandle kweengxelo zeziphumo ezingaguqukiyo, isampulu enye yayingalunganga emva kokurhoxisa i-LUSEAte enye, kodwa yayilungile kwi-Abbott Sars-2 kunye ne-Biotech yeBiotech-2019. Oku kuphakamisa ukuba kunokubakho ukwahluka kweziphumo kwiindidi ezahlukeneyo ze-assay. Nangona kunjalo, kwisifundo esenziwe e-China15, isiphumo se-Daan Fele Gene Gene Gene yayihluke kakhulu (p <0.05) xa kuthelekiswa ne-Ares Recicredit yabo. Nangona kunjalo, kwisifundo esenziwe e-China15, isiphumo se-Daan Fele Gene Gene Gene yayihluke kakhulu (p <0.05) xa kuthelekiswa ne-Ares Recicredit yabo. I-те Не ме менее, исследаваниии кв китае15, резуьаеа аan от на от латорноторного этало этала. Nangona kunjalo, kwisifundo e-China15, iziphumo zohlalutyo lwe-daan gene yayihluke kakhulu (P <0.05) ukusuka kuhlalutyo lwelebhu.然而, 在中国进行的研究中 15, 大安基因检测的结果与其实验室定义的参考检测相比有显着差异 (p <0.05).然而, 在中国进行的研究中 15, 大安基因检测的结果与其实验室定义的参考检测相比有显着差 <0.05 I Однако геедиче11 савнению сгалоныалоны лабораторе тестомомомомомомомомомомомомомомомом Nangona kunjalo, kwisifundo e-China15, iziphumo zovavanyo lwemfuza kaDaan zahlukile kakhulu (P <0.05) xa kuthelekiswa novavanyo lwelebhu.Oku kungangqinelani kunokubangelwa kukuziva ngovavanyo lokurejista ukufumanisa i-SARS-2, kunye nezifundo kunokuba kubalulekile ukumisela unobangela.
Ukongeza, isifundo sethu siphonononge intsebenzo yokuthelekisa ye-SARS-2 BGI-2 BGI nge-CRS, ibonisa i-CRS, ibonisa isivumelwano esihle se-PRES (i-PPA = 97.9%), i-NPA = 100%), kunye nemvumelwano yepesenti yesini (i-OPA). ). = 98.8%). Amaxabiso ka-Kappa kaCohen abonisa isivumelwano esihle (k = 0.975). Izifundo eNetherlands16 naseChina15 zibonakalise iziphumo ezingaguqukiyo. Uvavanyo lwe-SARS-Cov-2 ye-BGI yi-gene (okanye i-orf1a / b) usebenzisa i-10 μl ye-μl yokwazisa / ukufumanisa i-E-Edute. Ngaphandle kwesivumelwano semibalo esifanelekileyo kunye neziphumo zethu zesalathiso, uhlalutyo luphoswe iisampulu ezimbini ezazithandabuzekayo (i-1.22%) yesampulu iyonke. Oku kunokuba neziphumo ezinkulu zeklinikhi zothumelo olusebenzayo kuzo zombini izigulana kunye noluntu.
Olunye uhlalutyo lokuthelekisa olubandakanyiweyo kolu phononongo yayiyi-biotech biotech ye-biotech-2019 RTR-PRCR (PRCR); Ipesenti yonke yomdlalo yayingama-96.3%. Amandla esivumelwano ayegqitywe yixabiso le-leappa, elaliyi-0.925, ebonisa isivumelwano esipheleleyo kunye ne-CRS. Kwakhona, iziphumo zethu ziyafana kwizifundo eziqhutywa kwiYunivesithi yaseMzantsi Afrika eNyshau, China, nakwiSebe leLabhoratri yeLabhoratri yeZibhedlele, i-Liuzhou City, China17. Nangona le midlalo mibini ingentla yabhalwa, uvavanyo lwe-Chi-Square (uvavanyo lweMacNAR) lubonise ukuba iziphumo ze-biotech biotech assey sele ithelekiswa ne-CRS (P <0.005). Nangona le midlalo mibini ingentla yabhalwa, uvavanyo lwe-Chi-Square (uvavanyo lweMacNAR) lubonise ukuba iziphumo ze-biotech biotech assey sele ithelekiswa ne-CRS (P <0.005). Несмота назше бтоисисичис (китерр biotech biotech имеет резльитисисс п атичисткич сраисю сравненю I-0,005). Nangona isivumelwano esifanelekileyo semibalo ngasentla sarekhodwa, uvavanyo lwe-Chi-Square (uvavanyo lwe-MCNAR) lubonise ukuba isiphumo se-bionch bioreech asment ebaluleke kakhulu ekuthetheni i-CRS (P <0.005).尽管记录了上述良好的统计一致性, 但卡方检验尽管 记录记录 了 上述 统计 统计 统计, 但但但但 检验 ((Mac 检验 检验 检验 检验 ,,:) 检测 检测 检测 与 与 Несмотрямеченое втатич (и стиаимую ртицссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссссскичччсски ртичцс ртжцу Ngaphandle kwesivumelwano semibalo esihle esaphawulweyo apha ngasentla, uvavanyo lwe-Chi-Square (uvavanyo lwe-MC zulu) lubonise umahluko obalulekileyo (P <0.005) phakathi kwe-binsech ye-biotech kunye ne-CRS.Iisampulu ezintandathu (i-3.66%) zafunyanwa ziba ziimpawu zobuxoki xa kuthelekiswa ne-CRS (itheyibhile yongezo 1); Oku kubaluleke kakhulu, ngakumbi unikezwe amandla okuhambisa intsholongwane. Le datha ingentla ixhasa le nqanaba lokufumana eliphantsi15.
Kolu phononongo, amaxabiso e-CT ayemiselwe kwiqonga ngalinye le-Assary kunye neqonga elifanelekileyo, kunye neyona nto iphantsi i-CT Ixabiso le-CT lixelwe kwi-Abbott sabott sars-2 assay. Esi siphumo sinokuhambelana nenkqubo yovavanyo lwemfuza ye-abbott yangaxeshanye yokufumanisa i-SARS-2. Ke ngoko, ngokoMfanekiso 1, 87.6% ye-Abbott sars-cov-2 yeziphumo ze-ct inamaxabiso apha ngezantsi 20. Ixabiso le-CT ngaphezulu kwama-30 alirekhodwanga. Ukongeza ekusetyenzisweni kuka-Abbott yefomati yokuvavanya i-SARS-cop-2, esi siphumo sinokuhambelana nomda ophantsi wokuqatshelwa (iikopi ezingama-32.5 ze-RNA / ml) 18, eziphindwe kathathu kunomda ophantsi weNkampani ye-100 ye-RNA / ml. ml) 19.
Olu phando lunemilinganiselo ethile: Okokuqala, asinazo iindlela ezisemgangathweni / zesalathiso [ezinjengovavanyo lwentsholongwane okanye ezinye iimvavanyo zelebhu (lda)] ngenxa yokunqongophala kwezixhobo. Okwesibini, zonke iimodeli ezisetyenziswa kolu phononongo yayingu-Nasopharyngeal Swabs, ngelixa iziphumo bezingasebenzi kwezinye iintlobo zesampulu, kunye nesithathu, ubungakanani besampulu yethu yayincinci.
Olu phononongo luthelekiswa nokusebenza kwe-RTT yeRTT-PRR-PCR ye-SARS-COV-2 usebenzisa iisampulu zeNasonsngealsngeal. Zonke izinto ezifunyenweyo zaye zaphantse zabakho ngokusebenzayo, ngaphandle kwe-bionsech ye-biotech. Ngaphandle koko, izinga le-postpotisige eliphantsi lichongiwe kwi-Asson Stensech Assay xa kuthelekiswa ne-CRS (P <0.05). Ngaphandle koko, izinga le-postpotisige eliphantsi lichongiwe kwi-Asson Stensech Assay xa kuthelekiswa ne-CRS (P <0.05). I-пезу того, в в п твненю с crs (P <0,05). Ukongeza, uvavanyo lwe-biotech lubonise ipesenti ephantsi kweziphumo ezintle xa kuthelekiswa ne-CRS (P <0.05).此外, 与 i-crs 相比, i-binred biotech 检测的阳性率较低 (p <0.05).此外, 与 i-crs 相比, i-binred biotech 检测的阳性率较低 (p <0.05). Коме того, авниз с crs (P <0,05). Ukongeza, i-biotech ye-biotech ye-biotech yayinexabiso eliphantsi lokuthelekiswa ne-POST kwi-CRS (P <0.05).Uhlalutyo lwe-biotech biotech ye-bio19 Okokugqibela, i-biotech ye-biotech ye-biotech (ruo) ifuna ukuqinisekiswa ngakumbi kokusetyenziswa e-Ethiopia, kwaye kufuneka kuthathelwe ingqalelo kuvavanyo kubavelisi abathile.
Uyilo lwesifundo sokuthelekisa lwenziwa kumaziko emveli amane kwi-Addis Ababa, isibhedlele sase-Eka Kotebebe, iZiko loNyango lweCawa yeCawa, kunye neSibhedlele saseMulcloosis, kunye neSt State kaPeter. Idatha yaqokelelwa phakathi kukaDisemba 1 nowama-31, 2020. Iindawo zonyango zolu phononongo zakhethwe ngabom ngokusisiseko kwinani labo eliphezulu kunye nokufumaneka kwamaziko amakhulu onyango esixekweni. Ngokufanayo, izixhobo, kubandakanya ne-Abi 7500 kunye ne-Abbott M2000 ye-M2000 ye-PRCr ye-PCR, kwaye zakhethwa ngokweengcebiso zabavelisi be-NAAT, kwaye uninzi lweelebhu zase-Ethiopia zisetyenziswa ubuncinci ubuncinci kuzo. Uvavanyo lwe-gene, i-abbott sars-2, uvavanyo lwe-binchch, kunye ne-SARS-2 ye-BG-2 ye-BGI eyenziwa ngexesha lesifundo).
Ukuvavanywa kwe-SARS-2 yenziwa ukusuka nge-1 ukuya kwi-30 kaDisemba 2020 kusetyenziswa i-3 ml yezothutho (i-VTM) (itekhnoloji ye-vtm) (i-Shenzhen, eChina) eyadlulayo kwi-Ephids. Iisampulu ze-NasonAryngeal zaqokelelwa ngabaqokeleli besandi esiqeqeshiweyo kwaye bathunyelwa kwi-Ephi kwiipakethe ezintathu. Phambi kokuba uhlale wedwa we-nucleid, isampulu nganye yabelwa inombolo eyahlukileyo yesazisi. Ukukhutshelwa kwenziwa kwisampulu nganye ngokukhawuleza ekufikeni kweencwadi zokuphuma kunye nokuzenzekelayo. Ke ngoko, ngokungenisa ngokuzenzekelayo kwe-abbott m2000, i-1.3 ml (kubandakanya i-0.8 Ml Inc yethamsane yesampulu ye-Abnott ye-Abnot ye-Abnot ye-ABBOTRY Inc (i-Abbott Molecular Inc. ) I-batch yeesampulu ezingama-96 [92, ulawulo oluthe kratya kunye nolawulo lweetemplate ezimbini (NTC)] zibandakanyiwe kwinkqubo iyonke (i-NTC)] ibandakanyiwe kwinkqubo iyonke (i-revrievation kwaye ihlolwe) ngexesha lesibini le-SARS-2. imigodi. Ngokufanayo, yokukhupha incwadana yemigaqo, sebenzisa iisampulu ezifanayo (zokukhutshwa ngokuzenzekelayo kunye nokufunyanwa). Yiyo loo nto, kuyo yonke le nkqubo, i-140 μl iisampulu zangaphantsi zaza zasuswa zisebenzisa i-Qiaamp Viaamp ye-viaamp ye-chienit (i-qigen GmbH, i-hielden) kwiibhendi ze-24 (ezibandakanya iisampulu ezingama-20, i-NTCs ezimbini kunye nee-NTCs ezimbini. I-Edutes ikhuphe ngesandla ikhutshiwe kwaye ifunyanwe kusetyenziswa i-ABI 7500 ye-birmal cutred isebenzisa i-SARS-Cov-2 BG-2 BGI ASSEAY, NE-DEANCHEAY ASTY.
Ukuhlanjwa okuzenzekelayo kunye nokuhlanjululwa kwe-SARS-2 i-villa i-villa ilandela umgaqo we-beadtic esebenzisa i-Abnott DNA ye-Abna ye-Abna ye-Abna ye-Abna. Ukusebenza kweesampulu kunye nokulungisa amasuntswana entsholongwane kwenziwa kusetyenziswa i-rithiment ye-guinidine engaphezulu kwe-gianidine I-RNA emva koko yahlulwe kwiprotein ngokokwahlukaniswa kwesigaba esiqinileyo usebenzisa i-sicaca, okt ityiwa ye-guinidium kunye ne-alkaline ityiwa kunye ne-alkaline ityiwa kunye ne-alkaline PH ye-buffer ikhuthaze i-asisica (i-SIO2). Inyathelo le-rinsing lisusa iiproteni kunye nenkunkuma ukuvelisa isisombululo esicacileyo. I-RNA ebonakalayo ikwadwa kwii-micropargatical ezisekwe kwi-MicroPlocals ye-Magnetic Plain20,21. Kwelinye icala, ukuhlanjwa kwendalo kunye nokuhlanjululwa kwe-RNA kwenziwa yindlela yekholamu ye-SPIN kusebenzisa i-Gentrifugation endaweni yokuma kwe-magnetic kunye nokwahlukana neentsholongwane.
Uvavanyo lokufumana ixesha le-ABR-2 Kule protocol, ukungasebenzi kwesampulu ngaphambi kokukhutshwa kwebhafu kwi-56 ° C nge-30 min. Emva kwentsholongwane, i-virus esebenzayo, ukuthutha i-nucleid yenziwa kwi-Abbott M2000 SP kwi-0.5 ml vtm usebenzisa inkqubo yokulungiselela isampulu ye-Abbott m2000 ye-DNA ye-Abnop. Ngokutsho komvelisi. I-Amlphation kunye nokufunyanwa kwenziwa kusetyenziswa i-Abbott M2000 ye-Abbott ye-M2000 ye-PCR ye-PRR, kunye nokufunyanwa kabini kwenziwa kwi-RDRP kunye n ges. I-ROX) kunye ne-vic p (i-proprieritary diye) yokuqatshelwa kunye nokufunyanwa kolawulo lwangaphakathi, kuvumela ukubonwa ngaxeshanye kuzo zombini iimveliso ze-Amlplist 19.
Indlela yokuchonga yokufunyanwa kwayo le krp isekwe kwi-Technologce Technology Rt-PRCR. I-Orf1a / B kunye neGeeli zazikhethwe njengemimandla egciniweyo ngetekhnoloji ye-DEAAN ukuze ifumane i-anution yendawo ekujoliswe kuyo. I-primers ekhethekileyo kunye ne-Fluorescent Persies (n i-Georescent (n i-Geeorescy (n geneees ebhalwe nge-Fally, i-Orf1a / B I-B I-CPEESS) yenzelwe ukufumana iisampulu. I-Eleent Eeenty Eeeent kunye noMxube ongumsebenzi wawulungiselelwe ngokongeza i-5 μl ye-20 μ ye-20 ye-Master ye-Master ye-25. I-Amiltication kunye nokufunyanwa kwenziwa ngaxeshanye kwi-Abi 750024 ye-PCR ye-PCR.
I-Orf1a / B kunye ne-NE n ifunyenwe kusetyenziswa i-biotech biorech ye-biotech ye-acid-2019 ye-nucleid ye-acid i kit Lungiselela i-proces ethile kwi-gene nganye ekukhetheni ijelo le-FIFT ye-Orf1a / B kunye nomjelo we-rox ye-gener. Kule kitso ye-assay, i-Eueent kunye noMxokelelwane Reagents yongezwa ngolu hlobo lulandelayo: Lungiselela i-30 μl master Master Master Regant Reagent kunye ne-20 μl. Ixesha lokwenyani le-PCR 750025 isetyenziselwa ukubekwa / ukufunyanwa.
Uvavanyo lwe-SARS-Cov-2 ye-BGI yi-fluorescent ye-fluorescent ye-rusorescent ye-RTRR-PRCR ekufunyanisweni kweCovid-19. Ingingqi yethagethi ibekwe kwi-Orf1a / B yengingqi ye-SARS-2 ye-genome, eyindlela enye yokufunyanwa kwemfuyo. Ukongeza, i-geneng geneng β-martin yindawo enethagethi yangaphakathi enethagethi. Umxube ongumGqiqweni ulungiselelwe ngokuxuba i-20 μl yomxube we-Master of Riggent kunye ne-10 μl yesampulu ye-RNA ekhutshiweyo kwiplate ye-rna kwiplate ye-RNA kwiplate ye-RNA kwiplate ye-RNA kwiplate ye-RNA. I-ABI ye-ABI 7500 yeFluorestice I-FCR ye-PCR ye-PCR ye-PCR ye-PCR ye-PCR ye-PCR ye-PCR ye-PFR. Zonke izinto ze-nucleid acid, iimeko ze-PCR ze-Assary nganye, kunye nokutolikwa kweziphumo zenziwa ngokomiyalelo yomenzi ochaphazelekayo (iTheyibhile 3).
Kolu hlalutyo lothelekiso, asisebenzisanga indlela esemgangathweni yesalathiso ukumisela isivumelwano sepesenti (esifanelekileyo, esingalunganga, kwaye siyonke) kunye nolunye paramitha olwenziwe uhlalutyo. Uvavanyo ngalunye lovavanyo lwenziwa nge-CRS, kolu phononongo i-CRS yamiselwa ngumgaqo "Nayo nayiphi na into elungileyo" kwaye iziphumo zisetyenziswe kakuhle, sisebenzise iziphumo ezimbini zovavanyo. Ukongeza, kwimeko yeCovid-19 yosulelo, iziphumo ezibi zobuxoki ziyingozi ngakumbi kuneziphumo ezilungileyo zobuxoki. Ke ngoko, ukuba sithi "sikhaliphile" ngokuchanekileyo ngokusemandleni akho kumphumo we-CRS, ubuncinci iimvavanyo ze-Assay ezimbini mazibe nezona zilungileyo, zithetha ukuba isiphumo esinye esifanelekileyo sinokuvela kwi-EUAS. Ngenxa yoko, kwezi zine zovavanyo, iziphumo ezibini okanye ezingaphezulu zovavanyo ezinika iziphumo ezifanayo zithathwa njengezithandayo okanye ezingalunganga18,27.
Idatha iqokelelwe kusetyenziswa iifom zokukhupha idatha, ukungena kwedatha kunye nohlalutyo lwenziwa kusetyenziswa isoftware ye-Excel kunye ne-SPSS VERSIT 31.0 ngeenkcukacha-manani ezichazayo. Isivumelwano sepesenti esifanelekileyo, esingalunganga, kwaye ipesenti epheleleyo yepesenti yahlalutywa, kwaye inqaku le-KAPPA lalisetyenziselwa ukumisela inqanaba lesivumelwano sendlela nganye ene-CRS. Iinqobo zokuziphatha zeKAPPA zitolikwa:
Ukucaciswa kokuziphatha kwenziwa kwiDyunivesithi yase-Addis Ababa kwaye zonke iiprothokholi zovavanyo kolu phononongo zamkelwa yiBhodi yeZenzo zeSayensi yase-Ethiopia. Inombolo yesalathiso yelayisensi ye-Ephics ye-Ephi / IRB-279-2020. Zonke iindlela zisetyenziswe ngokungqinelana neengcebiso kunye nezibonelelo zeSiseko seSizwe se-Itiopia ngokunyanga kweCovid-19. Ukongeza, imvume ebhaliweyo ebhaliweyo yafunyanwa kubo bonke abathathi-nxaxheba bafundayo ngaphambi kokuthatha inxaxheba kwesifundo.
Zonke idatha ezifunyenweyo okanye ezihlaziyiweyo kolu phononongo zibandakanyiwe kweli nqaku lipapashiweyo. Idatha exhasa iziphumo zolu phononongo ziyafumaneka kumbhali ofanelekileyo kwisicelo esifanelekileyo.
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