Iprothokholi yokufumanisa imvelaphi yezilwanyana zeBigfish

Ingxaki yokhuseleko lokutya iya isiba mandundu ngakumbi. Njengoko umahluko wexabiso lenyama usanda ngokuthe ngcembe, isehlo “sokuxhonywa kwentloko yeegusha kunye nokuthengisa inyama yenja” kwenzeka rhoqo. Ukurhanelwa bubuqhophololo bobuxoki kunye nokunyhashwa kwamalungelo asemthethweni abathengi kunye nomdla, ukunciphisa igama loluntu lokhuseleko lokutya, okukhokelela kwimpembelelo embi ekuhlaleni. Ukuqinisekisa ngcono ukhuseleko lokutya kunye nemveliso yokhuseleko lwemfuyo kwilizwe lethu, imigangatho yokuhlola ethembekileyo kunye neendlela zifuneka ngokukhawuleza.

Ngobuchule obuqhubekayo kunye nokuzingisa kwabaphandi, i-Bigfish iye yaphuhlisa ngokuzimeleyo ikiti yokufumanisa ephuma kwisilwanyana, ibonelela ngezisombululo eziphambili kunye nezikhawulezayo kubathengi bethu! Sikwaziva sinelunda kakhulu ngokukwazi ukunceda abathengi bethu basombulule iingxaki zabo.
Igama lemveliso: Ikiti yokufumanisa imvelaphi yezilwanyana (ihagu, inkukhu, ihashe, inkomo, igusha)
Uvakalelo oluphezulu: ubuncinane bokubona umda we-0.1%
Ukucaciswa okuphezulu: ukuchongwa ngokuchanekileyo kwazo zonke iintlobo "zenyama yokwenyani kunye nenkohliso", akukho-cross-reactivity
1, iSample processing
Iisampulu zihlanjululwe kabini ukuya kathathu nge-ethanol ye-70% kunye namanzi aphindwe kabini, aqokelelwa kwiityhubhu ze-centrifuge ezicocekileyo ze-50 ml okanye iingxowa ezivaliweyo ezicocekileyo kwaye zigcinwe emkhenkceni -20 °C. Iisampulu zahlulahlulwe zaba ngamacandelo amathathu alinganayo, kubandakanywa isampuli eza kuvavanywa, isampuli ehlaziyiweyo kunye nesampuli egciniweyo.
2. Ukukhutshwa kwe-Nucleic acid
Iisampulu zezicubu ziyomiswa kwaye zisikwe ngokucokisekileyo okanye zongezwe kwinitrogen engamanzi, emva koko zibe ngumgubo kudaka kunye nepestle, kwaye iDNA yezilwanyana itsalwa kusetyenziswa i-automatic.i-nucleic acid extractor + IMagpure Animal Tissue Genomic DNA Purification Kit.

(Iseti yokutsalwa kweLabhoratri)

3. Uvavanyo lokukhulisa
Uvavanyo lokukhulisa lwenziwa kusetyenziswa iBigfish elandelelanayo yexesha lokwenyani yokulinganisa i-PCR analyzer + ikiti yokufumanisa esekwe kwisilwanyana ukuze kubonwe ngokuchanekileyo ukuba inyama igqwethiwe na ngokweziphumo ezibi, ukukhusela ngcono amalungelo abathengi kunye nokhuseleko lokutya.

Imizekelo: Ikiti yoVavanyo lweMvelaphi yeZilwanyana (iigusha)