Iinkqubo zokwenyani ze-PCR zexesha le-PCR: Ukuphucula uphando kunye noqwalaselo

Iinkqubo ze-PCR zokwenyani ze-PCRUguqukele kwimimandla yebhayoloji yemolekyuli kunye nokuxilongwa ngokubonelela ngabaphandi kunye noochwephesha abanezixhobo ezinamandla zokuhlalutya i-acid acid. Itekhnoloji inokuthi ifumane kwaye icwangcise i-DNA okanye i-RNA ye-RNA okanye kwi-RNA ye-RNA ngexesha lokwenyani, esenza i-asethi exabisekileyo kusetyenziswa iintlobo ezahlukeneyo zophando kunye nezicelo zokuchonga.

Esona sibonelelo siphambili senkqubo ye-PCR yexesha le-PCR kukukwazi kwabo ukubonelela ngokukhawuleza, okhethiweyo. Iindlela zemveli ze-PCR Ifuna uhlalutyo lwe-Amellic, ezinokuthi zichithe ixesha kwaye zinengxaki. Ngokwahlukileyo, inkqubo ye-PCR yokwenene ye-PCR yenza ukuba abaphandi babeka esweni i-DNA okanye i-RNA, ngaloo ndlela bafumana ulandelelwano lwethagethi ngexesha lokwenyani. Oku akufaneli kuphela ixesha kodwa kunciphisa umngcipheko wongcoliseko kunye nempazamo yomntu, ukwenza itekhnoloji yokufumana iteknoloji efanelekileyo nethembekileyo yemolekyuli.

Kwiiseti zophando, i-PCR yokwenyani ye-PCR isetyenziselwa ngokubanzi uhlalutyo, i-genobing, kunye nokufunyanwa kwe-gesiali. Amanqanaba okulinganisa intetho yesifo se-gene ngexesha lokwenyani ephucule kakhulu ukuqonda kwethu iinkqubo ezahlukeneyo zebhayiloji kunye neendlela zesifo. Abaphandi banokusebenzisa i-PRCR ye-PRC abafundi ukuya kufunda iziphumo zonyango okanye iimeko ezahlukeneyo kwigama lesifo, ukubonelela ngokuqonda okuxabisekileyo kubume bezifo kunye neethagethi ezinokubakho.

Iinkqubo ze-PCR zexesha le-PCR ze-PCR ziluncedo kwizifundo ze-sunoty ukuya ngokukhawuleza nangokuchanekileyo zichonga ngokwahlukileyo kwindalo kunye ne-polymorphisms. Oku kubaluleke kakhulu kwiindawo ezinjenge-chirmacogragics kunye namayeza abucala, apho kwahluka ekuphembeleleni kwemfuzo kwiziyobisi kunye neerejimeni zonyango. Ngokusebenzisa itekhnoloji ye-PRCR ye-PCR, abaphandi banokuyisikrini ngokufanelekileyo iimpawu zemfuza ezinxulunyaniswa nemetabolism yeziyobisi, izifo ezithenjiweyo, kunye neziphumo zonyango.

Kwindawo yokuxilongwa, i-PCS-yexesha lokwenyani idlala indima ebalulekileyo ekufumaneni nasekuhloleni izifo ezosulelayo, izifo zemfuzo kunye nomhlaza. Imvakalelo ephakamileyo kunye ne-PCR ye-PCR yenze ukuba ibe liqonga elifanelekileyo lokuchonga iintsholongwane ezinje ngebhaktiriya kunye neentsholongwane kwiisampulu zekliniki. Oku kubaluleke kakhulu kuphando oluphumileyo kunye nokuhlola iinzame, apho ixesha elifanelekileyo kwaye lichanekile kwaye kuchanekile kwimithombo yosulelo kubalulekile kungenelelo lwempilo yoluntu.

Ukongeza, iinkqubo ze-PCR zexesha le-PCR zisetyenziswa ngokubanzi ekufumaneni isifo kunye nokubekwa kweliso kwizifo zemfuza kunye nomhlaza. Ngokujongisisa iipateni ezithile ze-gene okanye iipateni ezingaqhelekanga ze-gene, abantu banokuthi basebenzise ixesha lokwenyani ukuncedisa ekuqaleni, i-progunosis, kunye nempendulo yonyango kwezifo ezahlukeneyo zemfuza nezeziseko. Ukuphucula kakhulu ukhathalelo lwezigulana ngokunika amandla onyango olwenziwe ngumntu kunye noluntu olusekwe kwiimpawu zemolekyuli zezifo zomntu ngamnye.

Njengoko itekhnoloji ye-PCR yexesha le-PCR iyaqhubeka, inkqubela-phambili efana ne-PCRX ye-PCR kunye neDijithali ye-Digital iyaphucula uphando lwayo kunye nokuxilongwa. I-Index-I-PRCRX ye-PRCr inokuthi ifumane ulandelelwano lwethagethi ngaxeshanye kwimpendulo enye, ukwandisa ubungakanani bohlalutyo lwe-molecular kunye nokugcina izinto zesampulu. Idijithali PCR, kwelinye icala, ibonelela ngokuthi ze-icids zenkumbulo ngokusasaza iimolekyuli ezithile kumawaka amagumbi okuphendula, ukubonelela ngovelwano olungenakuthelekiswa nanto kunye nokuchaneka.

Isishwankathelo,Iinkqubo ze-PCR zokwenyani ze-PCRYiba sisixhobo esibalulekileyo sokuphucula izakhono zophando kunye noxilongo kwi-molecular biology kunye nonyango lweklinikhi. Ukukwazi kwabo ukubonelela ngokukhawuleza, ngokuchanekileyo nangokuchanekileyo kunye nohlalutyo lwe-Acid iguqule ukuqonda kwethu iinkqubo zebhayoloji kunye neendlela zesifo kwaye uphuculwe ukuxilongwa nokulawulwa kweemeko ezahlukeneyo zempilo. Njengoko itekhnoloji iqhubeka ingenayo, i-FCE-yexesha lokwenyani