I-Revolutionizing PCR: I-FastCycler Thermal Cycler

Kwinkalo yebhayoloji yemolekyuli,iibhayisekile ezishushuzizixhobo eziyimfuneko kubaphandi nakwizazinzulu. Badlala indima ebalulekileyo kwinkqubo ye-polymerase chain reaction (PCR), esisiseko sokukhulisa i-DNA, i-cloning kunye nohlalutyo olwahlukeneyo lwemfuzo. Phakathi kweebhayisekile ezininzi ezishushu kwimarike, iFastCycler igqame ngeempawu zayo eziphambili kunye nezixhobo ezikumgangatho ophezulu, ibe ngumzekelo wokutsha kunye nokusebenza kakuhle.

Kwintliziyo ye-FastCycler kukuzibophelela kwayo kumgangatho, usebenzisa i-premium Peltier elements ezivela eMarlow, eU.SA. Ezi zinto zaziwa ngokuthembeka nokusebenza kwazo, okwenza i-FastCycler ifikelele kumazinga obushushu obumangalisayo ukuya kuthi ga kwi-6°C/S. Oku kukwazi ukukhwela ngokukhawuleza kubalulekile ekunciphiseni ixesha elifunekayo lokuhamba ngebhayisikile ye-PCR, ukuvumela abaphandi ukuba bafumane iziphumo ngokukhawuleza ngaphandle kokubeka esichengeni ingqibelelo yovavanyo.

Enye yeempawu zokuma kwe-FastCycler lubalo lomjikelo onomtsalane, odlula umjikelo we-100 lezigidi. Oku kuhlala kuthetha ukuba abaphandi banokusebenzisa i-FastCycler ixesha elide, okwenza kube yindleko-mali yotyalo-mali kwiilabhoratri ezifuna ukuqhubeka nokuphindaphinda ukuhamba ngebhayisikili ye-thermal. Ubomi obude be-FastCycler bubungqina boyilo lwayo kunye nobunjineli, iqinisekisa ukuba inokumelana nobunzima bokusetyenziswa kwelabhoratri yemihla ngemihla.

Ukuchaneka kweqondo lokushisa kubalulekile kwizicelo ze-PCR, kwaye i-FastCycler iyagqwesa kulo mba. Ngokufudumeza okuphezulu kwe-thermoelectric kunye nokupholisa iteknoloji edibeneyo kunye ne-PID (i-proportional-integral-derivative) yokulawula ubushushu, i-FastCycler igcina izinga eliphezulu lokuchaneka kweqondo lokushisa kuyo yonke inkqubo yebhayisikili. Oku kuchaneka kubalulekile ekufezekiseni ukukhulisa i-DNA, njengoko nokuphambuka okuncinci kubushushu kunokukhokelela kwiziphumo ezibi okanye ukusilela kovavanyo.

Ukufana kuwo onke amaqula ngomnye umba obalulekileyo wokuhamba ngebhayisekile eshushu, kwaye iFastCycler ayidanisi. Uyilo lwayo luqinisekisa ukuba zonke iisampuli ziyafudunyezwa kwaye zipholile ngokuqhubekayo, okubaluleke kakhulu kwiimvavanyo ezifuna iimeko ezifanayo. Oku kufana kunciphisa ukuguquguquka kwiziphumo, ukunika abaphandi ukuzithemba ukuba idatha yabo inokuthenjwa kwaye iyakwazi ukuveliswa kwakhona.

Ukongeza, i-FastCycler isebenza kumanqanaba engxolo aphantsi, iyenza ilungele indawo zelebhu ezifuna umoya ozolileyo. Olu phawu aluphuculi kuphela iimeko zokusebenza zabaphandi, kodwa lukwenza ukuba amava elebhu agxininise kwaye asebenze kakuhle.

Ngamafutshane, iI-FastCycler Thermal Cyclerimele intuthuko enkulu kwitekhnoloji yePCR. Ngezinto zayo ezikumgangatho ophezulu wePeltier, iirempu ezikhawulezayo, isalathiso esihle kakhulu sokuhamba ngebhayisikile, kunye nendlela yokulawula ubushushu obuphezulu, iyilelwe ukuhlangabezana neemfuno zophando lwebhayoloji yale mihla. Nokuba wenza ngokwesiqhelo i-DNA yokukhulisa okanye ubandakanyeka kuphando oluntsonkothileyo lwemfuza, iFastCycler ihambisa ukusebenza okuphezulu, ukuthembeka, kunye nokusebenza kakuhle. Ukutyala imali kwi-FastCycler kuthetha ukutyala imali kwikamva lophando lwakho, uqinisekisa ukuba unezixhobo ozifunayo ukutyhala imida yokufunyanwa kwesayensi.