Indaleko yomjikelezo we-thermal: Inguqu kwi-DNA ye-DNA

Abajikelezi bomzimbaYiba sisixhobo esibalulekileyo kubaphandi kunye nezazinzulu kwicandelo lebhayoloji yemolekyuli kunye nemfuza. Esi sixhobo sinobuchule siye saguqula inkqubo ye-DNA ye-DNA, iyenza ngokukhawuleza, isebenze kwaye ilunge ngakumbi kunangaphambili. Kwezi bhlog, siya kuphonononga ukuphuculwa komjikelo we-thermal kunye nefuthe labo kwicandelo lebhayiloji yemolekyuli.

Umxholo wokuhamba ngebhayisikile, obandakanya ukufudumeza okuphindaphindiweyo kunye nokupholisa umxube wokuphendula, sisiseko se-Polymerase Chain Reance (PCR). I-PCR yindlela yokukhuthaza iikopi enye okanye ezimbalwa zeDNA ngemiyalelo eliqela yobukhulu, ekuveliseni amawaka kwizigidi zeekopi zokulandelelana okukodwa. Ukuphuhliswa komjikelo we-thermal kudlale indima ephambili ekusebenziseni nasekuphuhlisweni kwetekhnoloji ye-PCR.

Abajikelezayo bokuqala be-thermal babeyi-bulky kunye ne-turkting amanyano kunye nokubeka esweni rhoqo. Nangona kunjalo, njengoko itekhnoloji ihambele phambili, umjikelo we-thermal wanamhlanje unesixhobo esinobuchule esinokuthi ulawule ngokuthe ngqo kubushushu obukhulu kwaye bazenze ngokuzenzekelayo. Olu phuculo luye lwandisa ngokukhawuleza isantya kunye nokusebenza kakuhle kwe-DNA ye-Ilpha ye-DNA, ivumela abaphandi benze lula i-PRR kwaye ngokuthembekileyo.

Enye yeendlela eziphambili ezinokubakho kwitekhnoloji ye-Irmal Winderrict yayikukuziwa kwe-PRCR PRCR, evumela amaqondo obushushu amaninzi anomdla aza kuvavanywa ngaxeshanye kuvavanyo olunye. Eli nqaku liqinisekisile ukuba luncedo kakhulu ekwenzeni iimeko zePCR yetemplate ethile ye-DNA, yongeza ixesha kunye nezixhobo.

Ukongeza, ukudityaniswa kwezakhono zexesha lokwenyani kwi-PCRc kumjikelo we-thermal usebenzise ukusetyenziswa kwawo. I-PCR ye-PCR, ekwabizwa ngokuba yi-PCR ye-PCR, ibeka i-DCR ye-DCR, ibeka i-DNA ye-DNA ye-DNA ngexesha lokwenyani, ibonelela ngengqondo ebalulekileyo kwinani lokuqala lokulandelelana kwe-DNA ekujolise kuyo. Oku kuneendawo eziguqukayo ezinje ngohlalutyo lwenkcazo ye-gene, i-genobing, kunye ne-pathogen.

I-mineathering yomjikelezo we-thermal iye yaba yinto ebalulekileyo kwiminyaka yakutshanje, iqhutywa yimfuno yokuphathwa kunye nokusebenza kakuhle. Ezi ziqulatho ze-compact, i-quards ye-thermal ephathekayo ifumene izicelo zophando lwasentsimini, ekuxilongeni lwe-Prestostics, kunye noSeto olulinganiselweyo lwezixhobo apho kusenziwa izibonelelo zelebhu zemveli.

Ukujonga phambili, ikamva leAbajikelezi bomzimbaSiza kubona nangakumbi. Iitekhnoloji ezikhulayo ezinje ngeendlela zedijithali zedijithali kunye ne-isothermal yokwaphula imida ye-DNA ye-DNA kwaye ibonelele ngezinto ezintsha zokuchongwa kunye nokuphuma ngokukhawuleza kwe-asidi.

Ngesishwankathelo, ukuphuculwa komjikelo we-thermal kuye kwanempembelelo enkulu kwintsimi ye-molecular biology, inkqubela phambili yokuqhuba kuphando, isifo, kunye ne-biotechnology. Ukusuka kwiibhloksi zokufudumeza eziya kuthi ga kwi-Hobles eziPhambili zanamhlanje, umjikelo we-thermal usebenzise i-DNA ye-DNA, esenza kube lula ukuyisebenzisa kwaye ithembeke ngakumbi kunangaphambili. Njengoko itekhnoloji iyaqhubeka ukuqhubela phambili, indima yomjikelo we-thermal ekubumbeni ikamva lekamva le-molecular iqinisekile ukuba ihlale ibalulekile.