Ukubaluleka kweeKhithi eziPhakathi zoThutho lweNtsholongwane kuQokelelo lweSampuli ye-Saliva

Kwinkalo ye-molecular diagnostics kunye nohlalutyo, ukuqokelela, ukugcinwa kunye nokuthuthwa kweesampuli ze-saliva yabantu ngamanyathelo abalulekileyo okuqinisekisa ukuchaneka nokuthembeka kweziphumo zovavanyo. Kulapho iikhithi zeViral Transport Media (VTM) zidlala indima ebalulekileyo. Ezi zixhobo zenzelwe ngokukodwa ukugcina ingqibelelo ye-viral nucleic acids ngexesha lokuthutha, okwenza kube sisixhobo esibalulekileyo kubasebenzi bezempilo kunye nabaphandi.

Umsebenzi ophambili weIkhithi yeVTMkukubonelela ngemekobume efanelekileyo yokugcinwa kweentsholongwane ze-nucleic acids ezikhoyo kwiisampuli zamathe. Oku kuphunyezwa ngokusebenzisa indlela yosasazo ekhethekileyo equkwe kwikhithi. Isixhobo esiphakathi sisebenza njengesithinteli esikhuselayo, sithintela ukuthotywa kwemathiriyeli yemfuzo yentsholongwane kunye nokuqinisekisa ukuzinza kwayo ngexesha lothutho oluya kwilebhu ukuze luhlalutywe ngakumbi.

Enye yeenzuzo eziphambili zokusebenzisa iikiti ze-VTM kukukwazi ukukhusela ingqibelelo ye-viral nucleic acids, evumela ukuxilongwa ngokuchanekileyo kwe-molecular kunye nokufumanisa. Iisampulu ezigciniweyo zinokuba phantsi kweendlela ezahlukeneyo zokuhlalutya, kubandakanywa ukukhulisa i-PCR kunye nokubhaqwa, ngaphandle kokuphazamisa umgangatho wezinto zofuzo. Oku kubaluleke kakhulu kuvavanyo lwesifo esosulelayo, apho ubukho bentsholongwane yentsholongwane kufuneka ichongwe ngokuchanekileyo kwaye ibonakaliswe.

Ukulungeleka kunye nokulula kokusetyenziswa kweVTM Kityenza ukuba ibe sisixhobo esiyimfuneko kubaboneleli bezempilo kunye nabaphandi ababandakanyekayo ekuqokeleleni iisampulu zamathe kunye nohlalutyo. Uhlobo olulungele ukusetyenziswa lwezikhithi luyenza lula inkqubo yokuqokelela isampulu kwaye iqinisekisa ukuba iisampulu zigcinwe ngokufanelekileyo kwaye zigcinwe de zifike elabhoratri. Oku akukongi ixesha kuphela kodwa kunciphisa umngcipheko wongcoliseko lwesampulu okanye ukuthotywa.

Ngaphezu koko, ukusetyenziswa kwe-VTM suite akuphelelanga kwizicwangciso zeklinikhi. Amaziko ophando kunye neelebhu zophononongo nazo zixhomekeke kwezi khithi ukuxhasa iinzame zabo zophando kunye noxilongo. Ukukwazi ukuthutha iisampulu zamathe ngokuzithemba nangokuthembeka kubalulekile ekuqhubeni izifundo ze-epidemiological, iinkqubo zokucupha, kunye neeprojekthi zophando ezijolise ekuqondeni amandla osulelo lwentsholongwane.

Isishwankathelo, ukubaluleka kweekhithi zeendaba zothutho lwentsholongwane ekuqokeleleni nasekuthuthweni kweesampulu zamathe abantu azikwazi ukugqithiswa. Ezi zixhobo zidlala indima ebalulekileyo ekugcineni ingqibelelo ye-viral nucleic acids, ngaloo ndlela iququzelela ukuxilongwa ngokuchanekileyo kwe-molecular kunye nohlalutyo. Njengoko imfuno yezixhobo ezithembekileyo zokuxilonga iqhubeka nokukhula, ii-VTM suites ziya kuhlala ziyinxalenye ebalulekileyo yokhathalelo lwempilo kunye nembonakalo yophando, igalelo ekuqhubeleni phambili kolawulo lwezifo ezosulelayo kunye namanyathelo ezempilo yoluntu.