Ukubaluleka kweekhilomitha eziphakathi zentsholongwane kwi-Nativang Coage

Kwintsimi yokuxilongwa kwe-molecular kunye nohlalutyo, ukuqokelelwa, ukugcinwa kunye nokuhamba kweesampulu zamahlalo abantu ngamanyathelo anqabileyo okuqinisekisa ukuchaneka kunye nokuthembeka kweziphumo zovavanyo. Kulapho kukho khona ii-chiel zothutho lweendaba (VTM) zekhithi zidlala indima ebalulekileyo. Ezi kokit ziyilelwe ngokukodwa ukugcina ingqibelelo yee-acids zenyukliya zenyukliya ngexesha lothutho, zibenzela isixhobo esibalulekileyo kwiingcali zempilo kunye nabaphandi.

Umsebenzi ophambili weI-VTM KitUkubonelela ngendawo efanelekileyo yokugcinwa kwee-acids zenyukliya zenyukliya ezikhoyo kwiisampulu ze-sativa. Oku kufezekiswa ngokusebenzisa iphakathi elikhethekileyo lokuhambisa kubandakanya ikhithi. I-Medium isebenza njenge-buffer ekhuselayo, ukuthintela ukonakaliswa kwezinto zofuzo zentsholongwane kunye nokuqinisekisa ukuba uzinzo kuhambo lwayo ukuya elabhoratri yohlalutyo.

Enye yezona zinto zibalulekileyo zokusebenzisa izixhobo ze-VTM kukwazi ukukhusela ukuthembeka kwee-acids zenyukliya i-acids, zivumela ukuba kuchongwe isifo semolekyuli echanekileyo kunye nokufunyanwa. Iisampulu ezigciniweyo zinokubekwa kwiindawo ezahlukeneyo zohlalutyo, kubandakanya nokukhutshwa kwe-PCR kunye nokufunyanwa, ngaphandle kokulalanisa umgangatho wezinto zofuzo. Oku kubaluleke kakhulu kuvavanyo lwezifo ezosulelayo, apho ubukho beentsholongwane zentsholongwane kufuneka zichongwe ngokuchanekileyo kwaye zibonakalise.

Ukulula kunye nokukhululeka kokusetyenziswa kweI-VTM KitYenza isixhobo esingazinzisiyo kubaboneleli bezempilo abakhathaleli kunye nabaphandi ababandakanyeka kwingqokelela yesampula kunye nohlalutyo. Uhlobo olulungele ukusetyenziswa kwezi kits zenze lula inkqubo yokuqokelela isampula kwaye iqinisekisa ukuba iisampulu zigcinwe ngokufanelekileyo kwaye zigcinwe de zifike elebhu. Oku kungagciniweyo kuphela kodwa kunciphisa umngcipheko wongcoliseko lwesampulu okanye ukonakaliswa.

Ngaphaya koko, ukusetyenziswa kwe-vtm suite akupheleli kwizicwangciso zeklinikhi. Amaziko ophando kunye neelebhu zokuxilonga zixhomekeke kwezi kiks zokuxhasa imizamo yazo yokuphanda. Ukukwazi ukuthutha iisampulu ze-lathu ngokuzithemba kwaye ngokuthembekileyo kubaluleke kakhulu ekuqhubeni izifundo zokwenza izifundo, iinkqubo zokuhlola, kunye neeprojekthi zophando ezijolise ekuqondeni amandla osulelo lwentsholongwane losulelo lwentsholongwane.

Ngesishwankathelo, ukubaluleka kweekhithi zemithombo yeendaba zentsholongwane kuqokelelo kunye nokuhamba kweesampulu zabantu abangenamandla akunakuba ngaphezulu. Ezi kits zidlala indima ebalulekileyo ekulondolozeni ukuthembeka kwee-acid zentsholongwane, ngaloo ndlela ziququzelela ukuxilongwa ngokuchanekileyo kwemolekyuli kunye nohlalutyo. Njengoko ibango lezixhobo zokuxilonga elinokuthenjwa liyaqhubeka nokukhula, amachaphaza e-VTM aya kuhlala eyinxalenye ebalulekileyo yempilo yonyamezelo kunye negalelo ekuqhubeni phambili kolawulo lwezifo ezosulelayo kunye namanyathelo oluntu.