Ukuguquguquka kunye nokubaluleka kwamacwecwe anzulu kwilabhoratri yanamhlanje

Kwilizwe elihlala liguquka lophando lwenzululwazi kunye nokulinga, izixhobo kunye nezixhobo ezisetyenziswa kwiilabhoratri zidlala indima ebalulekileyo kwimpumelelo yeeprojekthi ezahlukeneyo. Esinye isixhobo esiyimfuneko esinjalo yipleyiti yequla elinzulu. Ezi cwecwe zikhethekileyo ziye zaba yinto efunekayo kwiilabhoratri ezininzi, ngakumbi kwiinkalo ezifana nebhayoloji yemolekyuli, i-biochemistry, kunye nokufunyanwa kweziyobisi. Kule bhlog, siza kuphonononga ukuguquguquka kunye nokubaluleka kweepleyiti ezinzulu, usetyenziso lwazo, kunye neenzuzo abazizisa kubaphandi.

Yintoni ipleyiti yequla elinzulu?

A ipleyiti yequla elinzuluyi-microplate enoluhlu lwamaqula, ngalinye liyilelwe ukubamba imiqulu emikhulu yolwelo kune-microplate eqhelekileyo. Iipleyiti zamaqula anzulu ngokwesiqhelo zenziwe ngeplastiki ekumgangatho ophezulu kwaye ziza kwiindlela ezahlukeneyo zokuseta kunye namandla okusebenza ukusuka kwi-1 ml ukuya kwi-50 ml okanye ngaphezulu. La macwecwe enzelwe ukuvumela ukugcinwa kwesampulu esebenzayo, ukuxuba, kunye nokuhlalutya, okwenza zibe sisixhobo esibalulekileyo kwiindawo ezininzi zebhubhoratri.

Ukusetyenziswa kwepleyiti yequla elinzulu

Iipleyiti zequla elinzulu linoluhlu olubanzi lwezicelo, kubandakanywa kodwa kungaphelelanga apho:

1. Isampulu yoGcino: Abaphandi bahlala besebenzisa iipleyiti ezinzulu zokugcinwa kwexesha elide kwiisampulu zebhayoloji ezifana neDNA, iRNA, iiproteni, kunye neenkcubeko zeseli. Ubukhulu bomthamo wequla, isampuli ekhuselekileyo inokugcinwa ngaphandle komngcipheko wokunyuka kwamanzi okanye ukungcoliswa.
2. Ukuhlolwa komgangatho ophezulu: Ekufumaneni iziyobisi kunye nophuhliso, iipleyiti ezinzulu zibalulekile kwinkqubo yokuhlolwa kwe-high-throughput screening (HTS). Benza abaphandi ukuba bavavanye amawaka eekhompawundi ngaxeshanye, bekhawulezisa kakhulu ukuchongwa kwabantu abanokuba ngabagqatswa bamachiza.
3. I-PCR kunye ne-qPCR: Iipleyiti ezinzulu zisetyenziswa ngokuqhelekileyo kwi-polymerase chain reaction (PCR) kunye ne-quantitative PCR (qPCR) izicelo. Ziyilelwe ukuba zivumele ukuhamba ngebhayisikile ye-thermal esebenzayo kunye nokunciphisa umngcipheko we-cross-contamination phakathi kweesampuli.
4. Iprotein crystallization: Kwibhayoloji yolwakhiwo, iipleyiti ezinzulu zisetyenziselwa umfuniselo weprotein crystallization. Imingxuma emikhulu inika indawo eyaneleyo yokukhula kwekristale, nto leyo iyimfuneko kwizifundo zeX-reyi crystallography.
5. Inkcubeko yeSeli: Iipleyiti ezinzulu zikwasetyenziselwa ukwenza iiseli zenkcubeko kwindawo elawulwayo. Uyilo lwabo luvumela imigca yeeseli ezininzi ukuba ikhuliswe ngaxeshanye, iququzelele izifundo ezithelekisayo kunye nemifuniselo.

Izinto eziluncedo zokusebenzisa iipleyiti zequla elinzulu

Ukusebenzisa iipleyiti zequla elinzulu kubonelela ngeenzuzo ezininzi ezonyusa ukusebenza kunye nokuchaneka kwilabhoratri yakho:

1. Ukwandiswa kweSakhono: Olona ncedo luphambili lweepleyiti zequla elinzulu kukukwazi kwabo ukubamba umthamo omkhulu wolwelo, oluluncedo ngakumbi kuvavanyo olufuna isampulu enkulu.
2. Ukunciphisa umngcipheko wongcoliseko: Uyilo lwepleyiti enzulu lunciphisa umngcipheko wokungcola phakathi kweesampuli kunye nokuqinisekisa ukunyaniseka kweziphumo zovavanyo.
3. Ukuhambelana ne-Automation: Iipleyiti ezininzi ezinzulu zihambelana neenkqubo zokuphatha ulwelo oluzenzekelayo, ezivumela ukuhamba komsebenzi ophezulu kunye nokunciphisa impazamo yomntu.
4. Ii-Aplikeshini ezininzi: Njengoko bekutshiwo ngaphambili, iipleyiti zequla ezinzulu zinokusetyenziswa kwiintlobo ngeentlobo zezicelo, zizenze zibe sisixhobo esiguquguqukayo sabaphandi kwiinkalo ezininzi.
5. Isebenza kakuhle emalini: Ngokucubungula iisampuli ezininzi ngaxeshanye, iipleyiti ezinzulu zinokugcina ixesha kunye nezixhobo, ekugqibeleni zigcine iindleko zokusebenza zelabhoratri.

ukuququmbela

Ukuququmbela,iipleyiti zequla elinzuluziyinxalenye ebalulekileyo yokusebenza kwelebhu yanamhlanje. Ukuguquguquka kwabo, ukwanda kwamandla, kunye nokuhambelana ne-automation kubenza babe sisixhobo esixabisekileyo kubaphandi kwiinkalo ezahlukeneyo. Njengoko uphando lwezenzululwazi luqhubela phambili, ukubaluleka kwamacwecwe amaqula anzulu kuya kukhula kuphela, kuvula indlela yezinto ezintsha ezifunyenweyo kunye nezinto ezintsha. Nokuba ubandakanyeka ekubhaqweni kweziyobisi, kwibhayoloji yemolekyuli, okanye naluphi na olunye uqeqesho lwezenzululwazi, ukutyala imali kwiipleyiti zamaqula anzulu kunokuphucula kakhulu ubuchule bakho bophando.