Ukugxininisa kakhulu kunye nokubaluleka kweepleyiti ezinzulu kwilebhu yanamhlanje

Kwilizwe elihlala livela kuphando lwezenzululwazi kunye nokuvavanywa, izixhobo kunye nezixhobo ezisetyenziselwa iilebhu zidlala indima ebalulekileyo kwimpumelelo yeeprojekthi ezahlukeneyo. Isixhobo esinye esibalulekileyo sisiqwengana esinzulu. Ezi plates ezizodwa ziye zaba yi-ACE -E-kwiilebhu ezininzi, ngakumbi kumacandelo anje ngebhayoloji yemolekyuli, i-biochemists, kunye nokufunyanwa kweziyobisi. Kwezi bhlog, siya kuphonononga ukuqhubela phambili kwaye sibaluleka kwiipleyiti ezinzulu, izicelo zabo, kunye nezibonelelo abazizisa kubaphandi.

Yintoni ipleyiti enzulu?

A ipleyiti enzuluyi-microplate enothotho lwequla, nganye yenzelwe ukubamba imiqulu emikhulu yolwelo kune-microplate eqhelekileyo. Iipleyiti ezinzulu ezinzulu zidla ngokwenziwa ngeplastiki esemgangathweni ephezulu kwaye ziza ngeendlela ezahlukeneyo zokucwangciswa kwe-1 ml ukuya kwi-50 ml okanye ngaphezulu. Ezi plates zenzelwe ukuvumela ukugcinwa kwesampulu, ukuxuba, kunye nohlalutyo, kubenzela isixhobo esibalulekileyo kwizicwangciso ezininzi zelebhu.

UKUSETYENZISWA KWEMPILO YOBUCHULE

Iipleyiti ezinzulu ezinzulu zinoluhlu olubanzi lwezicelo, kubandakanya kodwa kungaphelelanga kuzo:

1. Ukugcinwa kwesampulu: Abaphandi bahlala besebenzisa iipleyiti ezinzulu zokugcina iisampulu zexesha elide kwiisampulu zebhayoloji ezinje ngeDNA, iRNA, iiproteni, kunye neenkcubeko zeseli. Elona lizwe likhulu, ukukhusela isampulu kunokugcinwa ngaphandle komngcipheko wokuphuma kwamanzi okanye ungcoliseko.
2. Uvavanyo lwegalelo eliphezulu: Kwindawo yokufumana iziyobisi kunye nophuhliso, iipleyiti ezinzulu kakhulu zibalulekile kwiNkqubo yokuNgena kweNkqubo (HTS). Benza ukuba abaphandi bavavanye amawaka eendawo eziqumzelayo ngaxeshanye, ekhawulezisa kakhulu ukuchongwa kwabagqatswa bafumanekileyo.
3. I-PCR kunye ne-QPCRIipleyiti ezinzulu ezinzulu zihlala zisetyenziswa kwi-Polymerase Chain Reance (PCR) kunye ne-PCRCR (QPCR). Ziyilelwe ukwenza ukuba ibhayisekile efanelekileyo ye-therming kunye nokunciphisa umngcipheko wokungcoliseka komnqamlezo phakathi kweesampulu.
4. Iprotein Crystalization: Kwibhayoloji yesakhiwo, iipleyiti ezinzulu-zisetyenziselwa iimvavanyo ze-protery protery. Imingxunya emikhulu ibonelela ngendawo eyaneleyo yokukhula kwekristali, ebaluleke kakhulu kwizifundo ze-X-ray ray story.
5. Inkcubeko yeseli: Iipleyiti zenzululwazi enzulu zisetyenziselwa iiseli zenkcubeko kwindawo elawulwayo. Uyilo lwabo luvumela imigca yeseli ezininzi ukuze ihlanjululwe ngaxeshanye, iququzelela izifundo zokuthelekisa kunye novavanyo.

Izibonelelo zokusebenzisa iipleyiti ezinzulu

Sebenzisa iipleyiti ezinzulu ezinzulu zinika izibonelelo ezininzi ezinyusa ukusebenza kakuhle kunye nokuchaneka kwilabhoratri yakho:

1. Ukunyuka komthamo: Eyona nto iphambili kwiipleyiti ezinzulu kakhulu kukukwazi kwabo ukubamba imiqulu emikhulu yolwelo, oluluncedo kakhulu kuvavanyo olufuna isixa esikhulu sesampulu.
2. Ukucutha umngcipheko wongcoliseko: Uyilo lwepleyiti enzulu inciphisa umngcipheko wokungcoliseka kwemilinganiselo kunye nokuqinisekisa ukuthembeka kweziphumo zovavanyo.
3. Ukuhambelana nokuzenzekelayoIipleyiti ezininzi ezinzulu ezinzulu zihambelana neendlela zokugcina izinto ezizenzekelayo, ezivumela ukulondolozwa kwendlela ephezulu kunye nokunciphisa amandla empazamo yomntu.
4. Izicelo eziguquguqukayo: Njengoko bekutshiwo ngaphambili, iipleyiti ezinzulu ezinzulu zinokusetyenziswa kwizicelo ezahlukeneyo, zibenzela isixhobo esiguquguqukayo kubaphandi ngeendlela ezininzi.
5. Isebenza kakuhle emalini: Ngokwenza iisampulu ezininzi ngaxeshanye, iipleyiti ezinzulu-zinzulu zinokugcina ixesha kunye nezixhobo, ekugqibeleni zisingisa iindleko zokusebenza kwamalabhoratri.

ukuququmbela

Ukuququmbela,Iipleyiti ezinzuluyinxalenye ebalulekileyo yokuqhelazwa kwelebhu yangoku. Ukusebenza kwabo jikelele, ukukhulisa kwabo amandla, kunye nokuhambelana nokuzenzekelayo kubenza isixhobo esibalulekileyo kubaphandi kwiindidi ezahlukeneyo. Njengoko uqoqosho lwezenzululwazi luyaqhubeka ukuqhubela phambili, ukubaluleka kweepleyiti ezinzulu kuya kukhula kuphela, ukutyhubela indlela yokufumanisa okutsha kunye nezinto ezintsha. Nokuba ngaba ubandakanyeka ekufumaneni iziyobisi, i-biology, okanye naluphi na olunye uqeqesho lwenzululwazi, ukutyala imali kwimilinganiselo ephezulu kakhulu kunokuphucula kakhulu amandla akho.