Ukutyhila amandla omjikelezo we-thermal: Isixhobo esiphambili kwi-biotechnology yanamhlanje

Kwimimandla ye-molecular biology kunye ne-bitechnology, abajikelezayo be-thermas zizixhobo ezinokuchazwa. Ihlala ibizwa ngokuba nguMatshini we-PCR, esi sixhobo sidlala indima ebalulekileyo yokwazisa i-DNA, esenza ukuba ibe lilitye lophando lwemfuzo, isifo, kunye nezicelo ezahlukeneyo zezamayeza nakwezolimo. Ukuqonda umsebenzi kunye nokubaluleka komjikelezo we-thermal kunokukhanyisa ifuthe labo kwinkqubela phambili yesayensi.

Yintoni umjikelezo we-thermal?

A umjikelezo we-thermalsisixhobo selebhu esisebenza i-polymerase chain yokuphendula (i-PCR). I-PCR yindlela esetyenziselwa ukukhuthaza amacandelo athile eDNA, avumela abaphandi ukuba bavelise izigidi zeekopi ngokulandelelana okukhethekileyo. Oku khupha kubalulekile kwizicelo ezahlukeneyo, kubandakanya uhlalutyo, kunye ne-ten bin, kunye nemfuza.
Imijikelezo ye-thermal isebenza ngothotho lwamaqondo obushushu, olubaluleke kakhulu kumanqanaba ahlukeneyo e-PCR. La manqanaba aquka ukwenziwa kwedayitile, ukungqubana, kunye ne-elongwation. Ngexesha lokuqulunqwa, i-DNA ephithizelekileyo kabini ishushu, isahlula kwimitya emibini. Iqondo lobushushu lihlisiwe ngexesha lesigaba esibonakalayo sokuvumela ukuba i-primers ibophelele kuluhlu lwe-DNA ekujolise kuyo. Okokugqibela, iqondo lobushushu liyaphakama kwakhona ukuba lingene kwinqanaba le-ElongngMerase iqokelele i-DNA entsha.

Iimpawu eziphambili zomjikelo we-thermal

Abajikelezi bomzimba banamhlanje baxhotyiswe ngeendlela ezahlukeneyo ezikhuthaza ukusebenza nokungasebenzi. Enye yezona nkqubela phambili zibalulekileyo kukukwazi ukwenza imijikelezo yamaqondo obushushu amaninzi, ivumela abaphandi benze ngokwezifiso iiprotocol zabo zePRR. Imijikelezo emininzi ye-thermal ikwabandakanya i-inid eshushu ethintela i-prosances ukuba isebenze kwii-tubs zokuphendula, ukuqinisekisa iimeko ezifanelekileyo zokwenza amandla.
Elinye inqaku elibonakalayo kukudityaniswa kokwenyani ukusebenza kwexesha le-PCR. Imijikelezo yexesha lokwenyani yenza ukuba abaphandi babeka esweni inkqubo ye-Amelphation ngexesha lokwenyani, ukubonelela ngedatha yobungakanani kwinani leDNA elivelisiweyo. Eli nqaku liluncedo ikakhulu kwizicelo ezinje nge-PCRCR (QPRCR), apho imilinganiselo echanekileyo ibaluleke kakhulu ukufumana iziphumo ezichanekileyo.

Ukusetyenziswa komjikelezo we-thermal

Izicelo zomjikelo we-thermal zibanzi kwaye zahluke. Kwisihlomelo seklinikhi, basetyenziselwa ukufumana ii-petogens, utshintsho lwendalo, kunye nezifo ezizuza njengelifa. Umzekelo, ngexesha leCovid-19 i-thendemic, abajikelezayo i-thermas ziye zadlala indima ephambili kwiisampulu zovavanyo ezikhawulezayo, zinceda ekuchongeni abantu abosulelekileyo kunye nokulawula ukusasazeka kwentsholongwane.
Kwiilebhu zophando, umjikelo we-thermal ubalulekile kwi-geneng gen, ukulandelelana, kunye ne-tem intetho. Bavumela oososayensi ukuba baphonononge ukwahluka kwemfuza kwaye baqonde iindlela zezinto ezisisiseko zesifo. Ukongeza, kwi-bietechnology yezolimo, abajikelezile i-thermal basetyenziselwa ukuphuhlisa izilwanyana eziguqulweyo zemfuza (i-GMOs) ezinokumelana noxinzelelo lwendalo esingqongileyo okanye uphucule umxholo wesondlo.

Ikamva loMjikelo we-Ormal

Njengoko itekhnoloji iyaqhubeka ivela, kunjalo, yenza umjikelezo we-thermal. Izinto ezintsha ezinokuba yimincili kunye nokudityaniswa namaqonga edijithali zikwindawo ethe tyaba. Ezi nkqubela zibalulekileyo zilindeleke ukuba zenze umjikelo we-therma afikeleleke ngakumbi kwaye zisebenziseka lula, zivumela abaphandi benze iimvavanyo ezisebenzayo kunye nokuchaneka.
Ukongeza, ukunyuka kwebhayiloji yebhayoloji kunye neyeza elenzelwe umntu ukuba iqhubele phambili kuphuhliso lwetekhnoloji yomjikelo we-thermal. Njengabaphandi bafuna ukuthoba izinto zemvelo, imfuneko yomjikelo we-thermas owuqhubileyo onamandla okutshintsha iiprofuculs ezintsonkothileyo ziya kunyuka kuphela.

ukuququmbela

Iumjikelezo we-thermal ingaphezulu kwesixhobo selebhu; Sisango lokuqonda ubunzima bobomi kwinqanaba lemolekyuli. Amandla ayo anyusa i-DNA anemimandla eguqukayo kwiyeza ukuya kwezolimo, esenza sisixhobo esibalulekileyo ekuqinisekiseni ulwazi kunye nezinto ezintsha. Ukujonga kwikamva, umjikelo we-thermal, ngokungathandabuzekiyo uya kuqhubeka nokudlala indima ephambili ekwakheni intsimi ye-biotechnology kunye nemolekyuli.