Ukuvula iimfihlo zoBomi: ukubaluleka kwe-nucleic stractions

Kwintsimi yebhayoloji yemolekyuli, ukukhutshwa kwee-acids ze-nucleic (i-DNA kunye ne-RNA) linyathelo elisisiseko, ukuvula indlela yezicelo ezingenakubalwa kuvavanyo lwezonyango. I-Nuleic Acid IKits ineekhithi ze-iNids iguqukele le nkqubo, esenza isebenze ngakumbi, inokuthenjwa, kwaye ifumaneke kubaphandi kunye neelebhu zehlabathi liphela. Kwezi bhlog, siya kuphonononga ezi chithi, ukubaluleka kwezinto zazo, kunye nefuthe lazo ekuqhubeni phambili kwesayensi.

Yintoni ikhithi yokukhuphela i-nucleic?

I-nucleic acid yokukhupha iikhilomithazizixhobo eziyilelwe ngokukodwa ukukhetha i-DNA okanye i-RNA kwiisampulu ezahlukeneyo zebhayoloji, ezinje ngegazi, izicubu, iiseli zendalo. Ezi kokiat zihlala zinazo zonke ii-regenta kunye neeprotokholi eziyimfuneko ukuqhuba inkqubo yokukhuphela, ukuqinisekisa ukuba abaphandi banokufumana i-acid esemgangathweni esemgangathweni enongcoliseko oluncinci.

Inkqubo yokukhuphela

Inkqubo yokukhupha iqhelekileyo ibandakanya amanyathelo amaninzi:

Iseli ye-lylisi: Inyathelo lokuqala kukuvula iiseli ukukhutshwa i-acid acid. Oku kudla ngokufezekiswa kusetyenziswa i-uffer ye-lysis enee-ocentments kunye nee-enzymes eziphazamisa iiproteni zeseli kunye neeproteni zentsholongwane.

Ukucocwa: Emva kokuba inyathelo le-acid i-nucleic likhutshiwe, inyathelo elilandelayo kukususa ungcoliseko olunje ngeeproteni, i-lipids, kunye nezinye iiseti zeselula. I-kits ezininzi zisebenzisa iikholamu ze-asisicals okanye iibhlorho zemagneti ukuya ekukhethekeni i-acid acids, ngokwenza oko ukungcola.

Isichazi: Ekugqibeleni, i-acid ecocekileyo ye-Acids icacile kwi-buffer efanelekileyo, ilungele izicelo ezisezantsi ezinjenge-PCR, ukulandelelana, okanye ukubekwa.

Kutheni usebenzisa ikhithi ye-nucleic acid?

Ukusebenza kakuhle: Iindlela ze-nucleic zemveli ze-acid zichitha ixesha kunye nokusebenza nzima. I-Nucleic Acid I-Kits Ects ​​IKITS EYENZA INKQUBO YENKQUBO YENKQUBO kwaye idla ngokugqibezela ukukhutshwa ngaphakathi kwiyure.

Ukungqinelana: Iiprotokholi ezimiselweyo ezinikezelwe zezi kits ziqinisekisa ukuzala kunye nokuthembeka kweziphumo. Oku kubaluleke kakhulu kuvavanyo apho ukuchaneka kubalulekile, njengokuxilongwa kweklinikhi okanye uphando.

Ukusebenza kwezinto eziphambili: Izixhobo ezininzi zenzelwe ukuphatha iintlobo ezahlukeneyo zeesampulu, zizenza zilungele izicelo ezahlukeneyo. Nokuba usebenza neesampulu zabantu, izicubu zezihlunu, okanye inkcubeko yentsholongwane, kusenokwenzeka ukuba kukho ikhithi ukuze ilungele iimfuno zakho.

Umsebenzisi onobuhlobo: Uninzi lwe-nucleid i-inkuridi ye-acid ye-scocid iza nemiyalelo eneenkcukacha kwaye yenzelwe ukuba ibe lula ukuyisebenzisa, nakwabo basenokungabinazimavana ezibanzi zelabhoratri. Oku kunengxaki yokufikelela kwiindlela zemolekyuli zemolekyuli, zivumela abaphandi abaninzi ukuba bathathe inxaxheba kuphando lwemitha.

UKUSETYENZISWA KOKUSETYENZISELWA KWE-INCLEIC

I-acid acid efunyenweyo kwezi kokia inokusebenza njengesiseko sezicelo ezininzi:

UPenqwa: Qho, uqonde umsebenzi we-gen, intetho kunye nommiselo.
UQEQESHO LWEZOBUCHULE
Inzululwazi ye-Forensic: Uhlalutyo lweesampulu zeDNA zophando lolwaphulo-mthetho.
I-biotechnology yezolimo: Ukuphuhliswa kwezinto zemveli eziguqulweyo zemfuza (GMOs) ukwandisa isivuno sesityalo.
ukuququmbela
I-nucleic acid yokukhupha iikhilomithaNgaba izixhobo ezingafunekiyo kwi-moleiology yanamhlanje, zivumela abaphandi ukuba bavule iimfihlo zobomi kwinqanaba lemolekyuli. Ukusebenza kwazo, ukungqinelana kwazo, kunye nokusebenza kube kanye kube kutshintsha imeko yophando kunye ne-viwotics, yenza kube lula kunangaphambili ukuphonononga ubunzima beDNA kunye neRNA. Njengoko itekhnoloji iyaqhubeka ukuqhubela phambili, sinokulindela ezi kokit ukuba zivele ngakumbi, ukuvula iminyango entsha yokufunyanwa kwesayensi kunye nezinto ezintsha. Nokuba ungumphengululi onamava okanye umtsha kwintsimi, ukutyala imali kwikhithi yokukhuphela esemgangathweni kunokuphucula umgangatho osemgangathweni wakho kunye negalelo kwiqumrhu elandayo lolwazi kwi-genetics.