Ukuvula iiMfihlo zoBomi: Ukubaluleka kweNucleic Acid Extraction Kits

Kwinkalo yebhayoloji yemolekyuli, ukutsalwa kwe-nucleic acids (i-DNA kunye ne-RNA) linyathelo elibalulekileyo, elivula indlela yezicelo ezingenakubalwa ukusuka kuphando lwemfuza ukuya kuxilongo lweklinikhi. Izixhobo zokutsalwa kwe-Nucleic acid ziyiguqule le nkqubo, ziyenza isebenze ngakumbi, ithembeke, kwaye ifumaneke kubaphandi nakwiilabhoratri kwihlabathi liphela. Kule bhlog, siza kuphonononga ezi khithi, ukubaluleka kwamacandelo azo, kunye nefuthe lazo kwinkqubela phambili yesayensi.

Yintoni ikhithi yokukhupha i-nucleic acid?

Iikhithi ze-Nucleic acid extractionzizixhobo ezenzelwe ngokukodwa ukwahlula i-DNA okanye i-RNA kwiisampulu ezahlukeneyo zebhayoloji, njengegazi, izicubu, iiseli, kunye neesampulu zokusingqongileyo. Ezi zixhobo ziqulethe zonke ii-reagents kunye neeprothokholi ezifunekayo ukuququzelela inkqubo yokukhupha, ukuqinisekisa ukuba abaphandi banokufumana i-nucleic acids esemgangathweni ophezulu kunye nokungcoliswa okuncinci.

Inkqubo yokutsalwa

Inkqubo yokutsalwa ibandakanya amanyathelo angundoqo aliqela: iseli lysis, ukucocwa, kunye elution.

I-Cell Lysis: Inyathelo lokuqala kukuvula iiseli ukukhulula i-nucleic acids. Oku kudla ngokufezekiswa kusetyenziswa i-lysis buffer equlethe izinto zokucoca kunye ne-enzymes eziphazamisa i-cell membranes kunye ne-denature proteins.

Ukuhlanjululwa: Emva kokuba i-nucleic acids ikhutshwe, isinyathelo esilandelayo kukususa ukungcola okufana neeprotheni, i-lipids, kunye nezinye i-debris zeselula. Iikiti ezininzi zisebenzisa iikholamu ze-silica okanye amaso azibuthe ngokukhetha ukubopha ii-nucleic acid, ngaloo ndlela zihlamba ubumdaka.

Elution: Okokugqibela, i-nucleic acids esulungekileyo ihlanjululwa kwisithinteli esifanelekileyo, esilungele usetyenziso olusezantsi olufana ne-PCR, ulandelelwano, okanye i-cloning.

Kutheni usebenzisa ikiti yokukhupha i-nucleic acid?

Ukusebenza kakuhle: Iindlela zemveli zokutsalwa kwe-nucleic acid zidla ixesha kwaye zisebenza nzima. Iikhithi zokutsalwa kwe-Nucleic acid zenza lula inkqubo kwaye zinokugqiba ukutsalwa ngeyure.

Ukungaguquguquki: Iiprothokholi ezisemgangathweni ezibonelelwa zezi khithi ziqinisekisa ukuveliswa kunye nokuthembeka kweziphumo. Oku kubalulekile kwimifuniselo apho ukuchaneka kubaluleke kakhulu, njengoxilongo lwezonyango okanye uphando.

I-Versatility: Iikiti ezininzi ziyilelwe ukuphatha iintlobo ezahlukeneyo zeesampulu, zizenza zilungele izicelo ezahlukeneyo. Nokuba usebenza neesampulu zabantu, izicubu zezityalo, okanye iinkcubeko ze-microbial, kunokwenzeka ukuba kukho ikiti ehambelana neemfuno zakho.

Umsebenzisi onobubele: Uninzi lweekhithi zokutsalwa kwe-nucleic acid ziza nemiyalelo eneenkcukacha kwaye ziyilelwe ukuba zibe lula ukuzisebenzisa, nakwabo basenokungenawo amava abanzi elabhoratri. Oku kufikelele kwidemokhrasi kubuchule bebhayoloji yemolekyuli, ukuvumela abaphandi abaninzi ukuba bathathe inxaxheba kuphando lwemfuzo.

Ukusetyenziswa kwe-nucleic acid extraction

I-nucleic acids efunyenwe kwezi kits inokusebenza njengesiseko sezicelo ezininzi:

Uphando lweGene: Qonda umsebenzi wemfuza, ukubonakaliswa kunye nommiselo.
I-Clinical Diagnostics: Ukufunyanwa kwezifo zofuzo, izifo ezosulelayo kunye nomhlaza.
INzululwazi yaseNzululwazi: Uhlalutyo lweesampulu zeDNA kuphando lolwaphulo-mthetho.
I-Agricultural Biotechnology: Ukuphuhliswa kwezinto eziphilayo eziguqulwe ngokwemfuza (GMOs) ukwandisa isivuno sezityalo.
ukuququmbela
Iikhithi ze-Nucleic acid extractionzizixhobo eziyimfuneko kwibhayoloji yale mihla yeemolekyuli, ezivumela abaphandi ukuba batyhile iimfihlelo zobomi kwinqanaba lemolekyuli. Ukusebenza kwazo, ukuguquguquka, kunye nokuguquguquka kwazo kuye kwatshintsha imeko yophando lwemfuza kunye noxilongo, nto leyo eyenza kube lula kunangaphambili ukuhlola ukuntsonkotha kwe-DNA kunye ne-RNA. Njengoko itekhnoloji iqhubeka nokuhambela phambili, sinokulindela ukuba ezi zixhobo zikhule ngakumbi, zivule iingcango ezintsha zokufunyanwa kwesayensi kunye nokuyila. Ingaba ungumphandi onamava okanye omtsha kwintsimi, utyalo-mali kumgangatho we-nucleic acid extraction kit unokuphucula kakhulu umgangatho womsebenzi wakho kwaye ube negalelo kumzimba oqhubekayo wolwazi kwi-genetics.