Ukuguquguquka kweePlate zeMithombo eNzulu kuPhando lweLebhu

Iipleyiti zemithombo enzuluzizinto ezibalulekileyo kuphando lwelabhoratri, zibonelela ngezisombululo eziguquguqukayo nezisebenzayo kwiintlobo ngeentlobo zezicelo. Ezi plate ze-multiwell zenzelwe ukwamkela iisampulu ngendlela ephucukileyo, nto leyo ezenza zibe sisixhobo esibalulekileyo kwiinkalo ezahlukeneyo zesayensi ezifana ne-genomics, i-proteomics, ukufunyanwa kwamayeza, nokunye.

Enye yezona nzuzo ziphambili zeepleyiti zemithombo enzulu kukukwazi kwazo ukuphatha ubungakanani obukhulu beesampuli. Ezi pleyiti zinobunzulu obuqala kwi-2 ukuya kwi-5 mm kwaye zinokuthwala ubungakanani besampuli ukuya kuthi ga kwi-2 ml ngomthombo ngamnye, nto leyo eyenza ukuba zilungele ukusetyenziswa okufuna ukucutshungulwa kobuninzi beesampuli. Olu phawu lubaluleke kakhulu kwiimvavanyo zokuhlola ezisebenzisa amandla aphezulu apho iisampuli ezininzi kufuneka zicutshungulwe ngaxeshanye.

Ukongeza kumthamo ophezulu weesampuli, iipleyiti zemithombo enzulu ziyahambelana nezixhobo ezahlukeneyo zelebhu, kubandakanya iinkqubo zokuphatha ulwelo oluzenzekelayo, ii-centrifuges, kunye ne-plate readers. Oku kuhambelana kuvumela ukuhlanganiswa okungenamthungo kwimisebenzi ekhoyo yelebhu, ukwenza iinkqubo zibe lula kunye nokusebenza kakuhle. Nokuba zisetyenziselwa ukulungiselela isampuli, ukugcina okanye uhlalutyo, iipleyiti zemithombo enzulu zibonelela ngeqonga elithembekileyo nelilungeleyo lokwenza iimvavanyo.

Ukongeza, iipleyiti ze-deep-well ziyafumaneka kwiifomathi ezahlukeneyo, kuquka ulwakhiwo lwe-96-, 384-, kunye ne-1536-well, nto leyo enika abaphandi ukuguquguquka ngokusekelwe kwiimfuno zabo zovavanyo. Oku kuguquguquka kwenza iipleyiti ze-deep-well zifaneleke kwiintlobo ngeentlobo zezicelo, ukusuka kwinkcubeko yeseli kunye nokukhula kweentsholongwane ukuya kwi-crystallization yeproteni kunye nokuhlolwa kwe-compound.

Uyilo lweepleyiti zemithombo enzulu lukwenza zilungele ukugcinwa nokugcinwa kweesampuli. Ukwakhiwa kwazo okuqinileyo kunye nokuhambelana kwazo neendlela zokutywina ezifana neefilimu zokuncamathelisa kunye neegasket zesiciko kuqinisekisa ukuthembeka kwesampuli kwaye kunciphisa umngcipheko wongcoliseko. Oku kwenza iipleyiti zemithombo enzulu zilungele ukugcinwa ixesha elide kweesampuli zebhayoloji, ii-reagents kunye neekhompawundi, okubonelela abaphandi ngesisombululo solawulo lweesampuli esithembekileyo.

Ukongeza, iipleyiti zemithombo enzulu ziyafumaneka kwizinto ezahlukeneyo, kuquka i-polypropylene kunye ne-polystyrene, nganye ineenzuzo ezizodwa kuxhomekeke kwindlela esetyenziswa ngayo. Umzekelo, iipleyiti zemithombo enzulu ye-polypropylene zaziwa ngokuchasana kwazo neekhemikhali kunye nokuhambelana kwazo neendidi ezahlukeneyo zezinyibilikisi, nto leyo ezenza zifaneleke ukusetyenziswa kweekhemikhali ezinobungozi. Iipleyiti zemithombo enzulu ye-polystyrene, kwelinye icala, zihlala zithandwa ngenxa yokucaca kwazo kokukhanya, nto leyo ezenza zilungele ukusetyenziswa okufuna ukuhlolwa ngokubonakalayo okanye ukufunyanwa kwe-fluorescence.

Isishwankathelo,iipleyiti zemithombo enzuluzizixhobo ezibalulekileyo kuphando lwelabhoratri, zibonelela ngokuguquguquka, ukusebenza kakuhle, kunye nokuthembeka kwiintlobo ngeentlobo zezicelo. Umthamo wazo ophezulu wesampulu, ukuhambelana nezixhobo zelabhoratri, kunye nokuguquguquka kwiifomathi kunye nezixhobo kuzenza zibe zizinto ezixabisekileyo kubaphandi kwiinkalo ezahlukeneyo zesayensi. Nokuba zezokucubungula, ukugcina okanye uhlalutyo lweesampulu, iipleyiti zemithombo enzulu ziyaqhubeka nokudlala indima ebalulekileyo ekuphuculeni ukufunyanwa kwesayensi kunye nobuchule.