Ikhithi yokuFumanisa i-SARS-CoV-2 Nucleic Acid (iFluorescence RT-PCR)

Inkcazo emfutshane:

Ikhithi yokufumanisa i-nucleic acid ye-SARS-COV-2 (i-Fluorescence RT-PCR) ingasetyenziselwa ukufumanisa i-nucleic acid ye-coronavirus entsha, isetyenziselwa ukuxilongwa okuncedisayo kunye nophando lwe-epid-emiological losulelo olutsha lwe-coronavirus, ifanelekile kwi-CDC, kwizibhedlele, kwilabhoratri yezonyango yomntu wesithathu, kwiziko lovavanyo lomzimba kunye nezinye iilabhoratri zeklinikhi.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zeMveliso

1, Uvakalelo oluphezulu: Umda woKufunyanwa (i-LoD)＜Iikopi ezi-2 × 102/ml.

2, I-gene ekujoliswe kuyo kabini: Fumana i-gene ye-ORFlab kunye ne-gene ye-N ngexesha elinye, thobela ummiselo we-WHO.

3, Ifanelekile kwizixhobo ezahlukeneyo: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; IBigFish-BFQP96/48 yethu.

4, Ikhawuleza kwaye ilula: I-reagent exutyiweyo kwangaphambili kulula ukuyisebenzisa, abathengi bafuna nje ukongeza i-enzyme kunye netemplate. Ikhithi yokukhupha i-nucleic acid yeBigfish ihambelana kakuhle nolu vavanyo. Ngokusebenzisa umatshini wokukhupha ozenzekelayo opheleleyo, kuyakhawuleza ukucubungula iisampulu ezininzi..

5, Ukhuseleko lweBio: I-Bigfish ibonelela ngeSampuli yoLwakhiwo oluSindayo ukuze ikhawulezise ukungasebenzi kwentsholongwane ukuqinisekisa ukhuseleko lwabaqhubi.