I-SARS-cov-2 i-nucleid ikhithi yokufumanisa (i-fluorescence rt-pcr)

Inkcazo emfutshane:

Kukho i-novel corona-vis primers kunye ne-fluorescent ekhethekileyo yeFluorescent kwinkqubo yokuphendula yekhithi. I-Novel Corona-Virus I-Acid ikhutshiwe yi-PCR Fluorescent Probe kwiNdlela yokuNgeniswa kwe-Vitro Apid, kwaye imiqondiso yeFluorescent ikhutshwe ngexabiso le-PRR, ukuze igwebe ngokukhawuleza iziphumo.

QHAGAMSHELANA NATHI

Iinkcukacha zemveliso

Iimpawu zeMveliso

Iimbonakalo

I-1, imvakalelo ephezulu: umda wokufumana (indawo) <2 × 102 Iikopi / ml

I-2, i-genes ezintathu ekujoliswe kuzo: i-orflab gene, n gene kunye nethagethi yangaphakathi ifunyenwe ngaxeshanye, ithobele ukuba ngubani ummiselo

I-3, ilungele izixhobo ezahlukeneyo: Abhi7500 / 75: 75: I-roche filycycyccler480; I-biorad cfx96; I-Bigqp-bfqp16 / 48

4, Ukukhawuleza kwaye kulula: Ukuphinda-phindwa kwangaphambili kulula ukuyisebenzisa, abathengi bafuna nje i-enzyme kunye netemplate. Ikhithi ye-nucleic ye-nucleic ye-nucleic ye-nucleid ihambelana ne-assay. Ngokusebenzisa umatshini opheleleyo ongezo ngokuzenzekelayo, kukhawuleza ukuqhubekeka neesampulu zevolumu ezinkulu.

I-5, i-Bio-Unution: I-Bigfish ibonelela ngesampula yokugcina isampulu ukuze isebenze ngokukhawuleza intsholongwane ukuze iqinisekise ngokhuseleko lwabasebenzi.

I-Amevation ijika le-SARS-cov-2

Ikhithi yokufumana i-nucleic

Isatifikethi se-CEVD

Igama lemveliso	Ikati.No.	Ukupakisha	Amanqaku
I-SARS-cov-2 i-nucleid ikhithi yokufumanisa (i-fluorescence rt-pcr)	I-BFRT06M-24m-24M-24	24T	Ubuntununtunu obuphezulu, kufanelekile kwiisampulu ezibuthathaka
	I-BFRT06M-48	48 I