Ikhithi yokuCoca ye-FFPE DNA

I-FFPE DNA yeKhiti yokuCoca eFakelwe umfanekiso

Inkcazelo emfutshane:

Ikiti isebenzisa isisombululo se-dewaxing esisingqongileyo ukukhupha i-DNA ye-genomic ephezulu kwiisampuli ezifakwe kwiparafini, ukoyisa umphumo wokuthintela obangelwa yi-formalin cross-linking.
Isampulu ixutywe kunye ne-cell lysis buffer ukuqhekeza iiseli kwaye iveze i-DNA. Isampulu ixutywe kunye ne-cell lysis buffer ukuphula iiseli kwaye iveze i-DNA. I-DNA evezwayo ibanjwe yi-nanoparticles ye-magnetic bead ukwenza i-bead-DNA complex. I-beads-DNA complexes zikhutshelwa kwi-beads-DNA complexes zitshintshelwa ukuhlamba i-buffer ukususa ukungcola okugqithisileyo. Okokugqibela, i-DNA ihlanjululwe kwaye ifunyenwe ngokuyidlulisela kwi-lution buffer.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Imizekelo

Amacandelo e-FFPE, iibhloko ze-FFPE, isampuli kwizisombululo zokulungisa ezifana ne-formalin

Amacandelo

Umzekelo	I-BFMP12R1	I-BFMP12R16	I-BFMP12R	Amacandelo
Ukupakisha	1T40T/ikhithi	16T16T/ikhithi	32T32T/ikhithi
UProtease K	400μL x 2	320μL	640μL	Isisombululo seProtease (Ipakishwe ngokwahlukeneyo)
RNase A	80μL	32μL	64μL	Isisombululo se-enzyme (Ipakishwe ngokwahlukeneyo)
Buffer FA	12mL	5ml	10ml	Isisombululo se-saline ephezulu
Buffer FL	Kumashumi amane anesithandathu-kakuhle ukulungiselela. ikhatriji 40 iipcs.	96 kakuhle ipakishwe kwangaphambili ipleyiti iipcs ezi-2	96 kakuhle ipakishwe kwangaphambili ipleyiti iipcs ezi-2.	I-denaturant eyomeleleyo kunye ne-Tris buffer
Buffer WA				Isisombululo se-saline ephezulu
Buffer WB				Isisombululo se-saline ephantsi
Isithinteli DE				Isisombululo se-saline ephantsi
Amaso magnetic				Isisombululo se-Hydroxy magnetic bead
Incwadi yabasebenzisi	1	1	1