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Ikhithi yokucoca i-genomic DNA yeMagapure FFPE
Intshayelelo emfutshane
Le khithi isebenzisa inkqubo ethile ye-buffer ephuhliswe kwaye ilungiselelwe kakuhle kunye neentsimbi zemagnethi ezibopha ngokukodwa kwi-DNA, ezinokubopha ngokukhawuleza, zifunxe, zahlule kwaye zicoce ii-nucleic acids. Indlela ekhethekileyo yokuqhekeza umhlwa isetyenziselwa ukuqhekeza nokukhulula i-DNA kumacandelo ezicubu, ukunciphisa umngcipheko wokonakala kwe-DNA okubangelwa kukudibanisa ii-molecular chain ezibangelwa yi-formalin-fixed tissue. Ngokuxhasa ukusetyenziswa kweBigfish Magnetic Bead Nucleic Acid Extractor, ifanelekile kakhulu ekukhupheni ngokuzenzekelayo ubungakanani obukhulu beesampuli. I-genomic DNA ekhutshiweyo inobunyulu obuphezulu kunye nomgangatho olungileyo, kwaye ingasetyenziswa ngokubanzi kwi-downstream PCR/qPCR, NGS kunye nolunye uphando lovavanyo.
Iimpawu zemveliso
◆Ikhuselekile kwaye ithembekile: Isebenzisa ulwelo olususa ulwelo olungcolisayo olunobuhlobo nokusingqongileyo, ayibandakanyi izinyibilikisi zendalo ezifana ne-xylene, kwaye ayinabungozi kwaye ayinabungozi.
◆Ikhawuleza kwaye ilula: Indlela ye-magnetic bead isetyenziselwa ukutsala nokucoca, ngeqondo eliphezulu lokuzenzekela kwaye akukho mfuneko ye-multi-step centrifugation.
◆Umgangatho olungileyo: I-DNA ye-genomic ekhutshiweyo inomlinganiselo ophezulu, ubumsulwa kunye nokuthembeka, kwaye ingasetyenziselwa ngokuthe ngqo kwiimvavanyo ezisezantsi.
Isixhobo esiguquguqukayo
Intlanzi enkulu iBFEX-32/BFEX-32E/BFEX-96E
Iiparameter zobugcisa
Umthamo wesampulu: izilayi ezi-5-8 ze-5-10 μm
Ubumsulwa be-DNA: A260/280≧1.75
Inkcazo yemveliso
| Igama lemveliso | Ikati. Hayi. | Ukupakisha |
| UMagaIcocekileI-FFPE GenomicIkhithi yokuCoca i-DNA(piphakheji egcwaliswe kwakhona) | I-BFMP12R | 32T |
| UMagaIcocekileI-FFPE GenomicIsixhobo sokuCoca i-DNA (iphakheji egcwaliswe kwangaphambili) | I-BFMP12R1 | 40T |
| UMagaIcocekileI-FFPE GenomicIsixhobo sokuCoca i-DNA (iphakheji egcwaliswe kwangaphambili) | I-BFMP12R96 | 96T |
| I-RNase A(pimpahla ethengiweyo) | BFRD017 | 1ml/ityhubhu (10mg/ml) |
Useto lwabucala