Ikhithi yokuCoca iDNA yeGenomic yeGagaPure Blood

Inkcazo emfutshane:

Le khithi iqulethe ii-microspheres ze-superparamagnetic kunye ne-buffer yokukhupha eyenziwe kwangaphambili, kwaye ifanelekile ekukhupheni i-DNA ye-genomic ngokulula nangokusebenzayo kwiisampuli zegazi elipheleleyo ezisandula ukugaywa, eziqandisiweyo, nezigcinwe ixesha elide. Iziqwenga ze-DNA ye-genomic ezikhutshiweyo zikhulu, zicocekile kakhulu, kwaye zinomgangatho ozinzileyo nothembekileyo. I-DNA ekhutshiweyo ifanelekile kwiimvavanyo ezahlukeneyo ezisezantsi ezifana nokugaya i-enzyme, i-PCR, ukwakhiwa kwelayibrari, i-Southern hybridization, kunye ne-high-throughput sequencing.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Uluhlu olubanzi lwezicelo zesampulu:I-DNA ye-genomic inokukhutshwa ngqo kwiisampuli ezifana negazi elichasene nokugaywa kwegazi (i-EDTA, i-heparin, njl.njl.), i-buffy coat, kunye namahlwili egazi.
Ngokukhawuleza kwaye kulula:i-sample lysis kunye ne-nucleic acid binding zenziwa ngaxeshanye. Emva kokufaka isampuli kumatshini, ukukhutshwa kwe-nucleic acid kugqitywa ngokuzenzekelayo, kwaye i-genomic DNA esemgangathweni ophezulu ingafunyanwa kwimizuzu engaphezu kwama-20.
Ikhuselekile kwaye ayinabungozi:Le reagent ayinazo izinyibilikisi ezinobuthi ezifana ne-phenol kunye ne-chloroform, kwaye ine-factor ephezulu yokhuseleko.

Izixhobo ezilungisekayo

Intlanzi enkulu iBFEX-32E/BFEX-32/BFEX-96E

Iiparameter zobugcisa

Ubungakanani besampulu:200μL
Imveliso ye-DNA:≧4μg
Ubumsulwa be-DNA:A260/280≧1.75

Inkcazo

Igama lemveliso	Ikati. Hayi.	Ukupakisha
Ikhithi yokuHlambulula iDNA yeGenomic yeGagaPure Blood Genomic (Iphakheji egcwalisiweyo kwangaphambili)	BFMP02R	32T
Ikhithi yokuHlambulula iDNA yeGenomic yeGagaPure Blood Genomic (Iphakheji egcwalisiweyo kwangaphambili)	BFMP02R1	40T
Ikhithi yokuHlambulula iDNA yeGenomic yeGagaPure Blood Genomic (Iphakheji egcwalisiweyo kwangaphambili)	BFMP02R96	96T