Ikhithi yokuHlambulula iDNA yeZilwanyana zeZilwanyana zeMagaPure

Inkcazo emfutshane:

Le mveliso isebenzisa inkqubo ye-buffer ekhethekileyo neyilungiselelwe ngokukodwa kunye neentsimbi zemagnethi ezibopha i-DNA ngokukodwa. Ingabopha, ifunxe, yahlukanise kwaye icoce ii-nucleic acids ngokukhawuleza. Ifanelekile ekukhupheni nasekucoceni i-genomic DNA ngokufanelekileyo kwiithishu ezahlukeneyo zezilwanyana kunye nezitho zangaphakathi (kuquka izinto eziphilayo zaselwandle). Ingasusa ukungcola okufana neeproteni ezahlukeneyo, amafutha kunye nezinye izinto eziphilayo kakhulu. Ixhotyiswe ngesixhobo sokukhupha i-nucleic acid se-BIGFISH Magnetic Bead Method, ifanelekile kakhulu ekukhupheni ngokuzenzekelayo iisampulu ezinkulu. Iimveliso ze-nucleic acid ezikhutshiweyo zinobunyulu obuphezulu kunye nomgangatho olungileyo, kwaye zingasetyenziswa kakhulu kwi-PCR/qPCR esezantsi, i-NGS, i-Southern hybridization kunye nolunye uphando lovavanyo.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Uluhlu olubanzi lwezicelo zesampulu:I-DNA ye-genomic inokukhutshwa ngqo kwiisampuli ezahlukeneyo zezilwanyana
Ikhuselekile kwaye ayinabungozi:Le reagent ayinazo izinyibilikisi ezinobuthi ezifana ne-phenol kunye ne-chloroform, kwaye ine-factor ephezulu yokhuseleko.
Ukuzenzekelayo:I-BIGFISH Nucleic Acid Extractor exhotyisiweyo inokwenza ukukhupha okuphezulu, ngakumbi okufanelekileyo ekukhupheni iisampulu ezinkulu
Ubunyulu obuphezulu:ingasetyenziswa ngokuthe ngqo kwi-PCR, ekugayweni kwee-enzyme, ekudibaniseni kunye nezinye iimvavanyo zebhayoloji yemolekyuli

Iinkqubo zokukhupha

I-MagaPure-Animal-Tissue-Genomic-DNA-Purification Kit

Imifanekiso yezicubu zezilwanyana - imifanekiso yegrinder kunye nemortar - imifanekiso yokuhlamba isinyithi - imifanekiso yesixhobo sokukhupha i-nucleic acid
Ukuthatha isampuli:Thatha izicubu zesilwanyana ezingama-25-30mg
Ukusila:ukugaya i-nitrogen engamanzi, ukugaya okanye ukusika
Ukugaya ukutya:Ukuhlamba okufudumeleyo okungama-56℃
Kumatshini:centrifuge uze uthathe i-supernatant, uyifake kwipleyiti yomthombo onzulu uze uyikhuphe kumatshini

Iiparameter zobugcisa

Isampuli:25-30mg
Ubumsulwa be-DNA:A260/280≧1.75

Isixhobo esiguquguqukayo

Intlanzi enkulu iBFEX-32/BFEX-32E/BFEX-96E

Inkcazo yemveliso

Igama lemveliso	Ikati. Hayi.	Ukupakisha
Ikhithi yokuHlambulula iDNA yeZilwanyana zeZilwanyana zeMagaPure (Iphakheji egcwalisiweyo kwangaphambili)	BFMP01R	32T
I-MagaPure Animal Tissue Genomic DNA Purification Kit (iphakheji egcwalisiweyo kwangaphambili)	BFMP01R1	40T
I-MagaPure Animal Tissue Genomic DNA Purification Kit (iphakheji egcwalisiweyo kwangaphambili)	BFMP01R96	96T
I-RNase A (Ukuthenga)	BFRD017	1ml/pc（10mg/ml）