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Ikhithi yokuHlambulula iDNA yeZilwanyana zaseMagaPure zasemanzini
Intshayelelo emfutshane
Le khithi isebenzisa inkqubo ethile ye-buffer ephuhliswe kakuhle nelungiselelweyo kunye neentsimbi zemagnethi ezibopha ngokukodwa kwi-DNA. Ingabopha ngokukhawuleza, ifunxe, yahlukanise, kwaye icoce ii-nucleic acids, kwaye yenzelwe ngokukodwa izilwanyana zasemanzini. Ifanelekile ngokukodwa ekukhupheni nasekucoceni i-genomic DNA ngokufanelekileyo kwiithishu ezahlukeneyo zezilwanyana zasemanzini, kwaye inokususa ukungcola okufana neeproteni, amafutha, kunye nezinye izinto eziphilayo kangangoko kunokwenzeka. Ngokuxhasa ukusetyenziswa kweBigfish Magnetic Bead Nucleic Acid Extractor, ifanelekile kakhulu ekukhupheni ngokuzenzekelayo ubungakanani obukhulu beesampuli. I-genomic DNA ekhutshiweyo inobunyulu obuphezulu kunye nomgangatho olungileyo, kwaye ingasetyenziswa ngokubanzi kwi-downstream PCR/qPCR, NGS, Southern hybridization kunye nolunye uphando lovavanyo.
Iimpawu zemveliso
◆ Iisampuli ezisetyenziswa kakhulu: I-DNA ye-genomic ingakhutshwa ngqo kwiisampuli ezahlukeneyo zezilwanyana zasemanzini
◆ Ikhuselekile kwaye ayinatyhefu: I-reagent ayinazo izinyibilikisi ezinobuthi ezifana ne-phenol kunye ne-chloroform, kwaye inokhuseleko oluphezulu.
◆ Okwenziwa ngokuzenzekelayo: Ixhotyiswe ngeBigfish Nucleic Acid Extractor, inokwenza ukukhupha okuphezulu, okufanelekileyo ngakumbi ekukhupheni ubungakanani obukhulu beesampuli
◆ Ubunyulu obuphezulu: bungasetyenziswa ngokuthe ngqo kwiimvavanyo zebhayoloji yemolekyuli ezifana ne-PCR, ukugaywa kwee-enzyme, ukuxutywa, njl.
Iinkqubo zokukhupha
Ukuthatha iisampulu: Thatha i-25-30 mg yezicubu zezilwanyana zasemanzini
Ukusila: ukusila i-nitrogen engamanzi, ukusila okanye ukucheba
Ukugaya: 56℃ ukugaya okufudumeleyo ebhafini
Kumatshini: Faka i-centrifuge kwi-supernatant uze uyifake kwipleyiti ukuze uyikhuphe
Iiparameter zobugcisa
Isampuli yesage: 25-30mg
Ubumsulwa be-DNA: A260/280≧1.75
Isixhobo esiguquguqukayo
Intlanzi enkulu iBFEX-32/BFEX-32E/BFEX-96E
Inkcazo yemveliso
| Igama lemveliso | Ikati. Hayi. | Ukupakisha |
| UMagaIcocekileI-Genomic yezilwanyana zasemanziniIkhithi yokuCoca i-DNA(piphakheji egcwaliswe kwakhona) | I-BFMP21R | 32T |
| UMagaIcocekileI-Genomic yezilwanyana zasemanziniIsixhobo sokuCoca i-DNA (iphakheji egcwaliswe kwangaphambili) | I-BFMP21R1 | 40T |
| UMagaIcocekileI-Genomic yezilwanyana zasemanziniIsixhobo sokuCoca i-DNA (iphakheji egcwaliswe kwangaphambili) | I-BFMP21R96 | 96T |
| I-RNase A(pimpahla ethengiweyo) | BFRD017 | 1ml/ityhubhu (10mg/ml) |
Useto lwabucala