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I-Spaniatity ye-Aqumicic Aqumic DNA Ukuhlanjwa kweKit
Intshayelelo emfutshane
Le khiti yamkela i-Buffer ye-Buffer eyodwa kunye ne-bufficis ekhethekileyo kunye neebhlorho ezibonakalayo ezibophelela kwiDNA. Inokubopha ngokukhawuleza, i-adsorb, yahlukile, kwaye ihlambulule i-acid acids, kwaye yenzelwe ngokukodwa izilwanyana zasemanzini. Ilungele ngokukodwa kwi-DNA efanelekileyo kwaye icoceke i-DNA ye-gemic ukusuka kwizicubu zezilwanyana zasemanzini, kwaye inokususa izinto ezinqabileyo ezinjengeeproteni, amanqatha, kunye nezinye izinto zezinto eziphilayo ukuya kwinqanaba eliphezulu. Ngokuxhasa ukusetyenziswa kwe-Bignetish Bednetic Bead Acid I-Acleid I-Acceractor ye-Acleid, ilungele ukuthotyelwa okuzenzekelayo kweesayizi ezinkulu zesampulu. I-DNA ekhutshiweyo inomgangatho ophezulu kunye nomgangatho olungileyo, kwaye inokusetyenziswa ngokubanzi kwi-DCR / QPCR, i-NGS, i-ngridance yovavanyo.
Iimpawu zemveliso
Iisampulu ezisebenzayo ngokubanzi: I-DNA ye-gemic inokuthi ikhutshwe ngqo kwiisampulu zesilwanyana ezahlukeneyo zasemanzini
◆ Kukhuselekile kwaye kungenetyhefu: Ukuqeshisa akunazo iityhefu ezinetyhefu ezinje nge-phenol kunye ne-chloroform, kunye nento ekhuselekileyo yokhuseleko
I-Automation Automap: Ixhotywe nge-nucleid ye-nucleid ye-nucleid ye-acid, inokwenza ukhuphelo lwegalelo eliphezulu, ngakumbi ngokufanelekileyo ekukhutsheni ubungakanani besayizi
Ucoceko oluphezulu: Inokusetyenziselwa ngqo i-molecular bients enje nge-PCR, i-enzyme yokugaya, i-hyridation, njl.
Iinkqubo zokukhupha
Isampulu: thatha i-25-30 mg yezicubu zesilwanyana zaseAquatic
Ukugaya: I-nitrogen i-nitrogen grinding, i-grinder yokugaya okanye yokucheba
Ukugaya: 56 ℃ Diginotion efudumeleyo
Kumatshini: I-Centrifuyifu i-supernatant kwaye yongeze kwiplate yokukhupha
Iiparamitha zobugcisa
Isampulu yesampulu yesampulu: 25-30mg
I-DNA Puill: A260 / 280 ≧ 1.75
Isixhobo esifanelekileyo
I-Bigfish Bfex-32 / BFEX-32E / BFEX-96E
Ukucaciswa kwemveliso
| Igama lemveliso | Ikati. Hayi. | Ukupakisha |
| MagaIcocekileI-Aquatic yezilwanyanaIkhithi yokucoca i-DNA(pIphakheji ehlaziyiweyo) | I-bfmp21R | 32t |
| MagaIcocekileI-Aquatic yezilwanyanaIkhithi yokucoca i-DNA (iphakheji yangaphambili) | I-bfmp21R1 | 40T |
| MagaIcocekileI-Aquatic yezilwanyanaIkhithi yokucoca i-DNA (iphakheji yangaphambili) | I-bfmp21R96 | I-96t |
| Rnase a(pu-urchase) | Bfrd017 | 1ml /ityhubhu (I-10mg / ml) |
Useto lwabucala