Ikhithi yokuCoca iDNA yeMagaPure Oryza sativa L. Genomic

Inkcazo emfutshane:

Le khithi isebenzisa inkqubo ethile ye-buffer ephuhliswe kakuhle nelungiselelweyo kunye neentsimbi zemagnethi ezibopha ngokukodwa kwi-DNA, ezinokubopha ngokukhawuleza, zifunxe, zahlukanise kwaye zicoce ii-nucleic acids, ngelixa zisusa ngokufanelekileyo ukungcola okufana nee-polysaccharides kunye nee-polyphenol complexes kwizityalo. Ifanelekile kakhulu ekukhupheni i-genomic DNA kwizicubu zamagqabi ezityalo. Ngokuxhasa ukusetyenziswa kweBigfish Magnetic Bead Nucleic Acid Extractor, ifanelekile kakhulu ekukhupheni ngokuzenzekelayo ubungakanani obukhulu beesampuli. I-genomic DNA ekhutshiweyo inobunyulu obuphezulu kunye nomgangatho olungileyo, kwaye ingasetyenziswa kakhulu kwi-downstream PCR/qPCR, NGS kunye nolunye uphando lovavanyo.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Intshayelelo emfutshane

Iimpawu zemveliso

◆ Ikhuselekile kwaye ayinabungozi: Akukho mfuneko yee-reagents ze-organic ezinobungozi ezifana ne-phenol/chloroform
◆ I-automated high-throughput: Ixhotyiswe ngeBigfish Nucleic Acid Extractor, inokwenza i-high-throughput extraction kwaye ifanelekile ekukhupheni ubungakanani obukhulu besampulu.
◆ Ubunyulu obuphezulu kunye nomgangatho olungileyo: Imveliso ekhutshiweyo inyulu obuphezulu kwaye ingasetyenziselwa i-NGS esezantsi, i-chip hybridization kunye nezinye iimvavanyo.

Iinkqubo zokukhupha

Ukuthatha iisampulu: Isampuli entsha malunga ne-100 mg okanye isampuli yobunzima obomileyo malunga ne-30 mg
Ukusila: Sila ngokupheleleyo nge-nitrogen engamanzi okanye nge-grinder
Ukugaya: 65℃ ukuhlamba okufudumeleyo
Kumatshini: Faka i-centrifuge kwi-supernatant uze uyifake kwipleyiti ukuze uyikhuphe

Isixhobo esiguquguqukayo

Intlanzi enkulu iBFEX-32/BFEX-32E/BFEX-96E

Inkcazo yemveliso

Igama lemveliso	Ikati. Hayi.	Ukupakisha
UMagaIcocekileU-Oryza sativa L.Ikhithi yokuCoca i-DNA yeGenomic(piphakheji egcwaliswe kwakhona)	I-BFMP23R	32T
UMagaIcocekileU-Oryza sativa L.Isixhobo sokuCoca i-DNA yeGenomic (iphakheji egcwaliswe kwangaphambili)	I-BFMP23R96	96T
I-Proteinase K (p)impahla ethengiweyo)	I-BFRD007	1ml/ityhubhu (10mg/ml)
I-RNase A(pimpahla ethengiweyo)	BFRD017	1ml/ityhubhu (10mg/ml)