I-Pagmire iyonke yokucoca i-RNA itota

I-PINPPURE iyonke i-rana yokucoca i-RNA yeKit

Inkcazo emfutshane:

Le khiti yamkela i-Buffer ye-Buffer equleneyo kwaye iyodwa eyahlukileyo ebophelelanayo kwiDNA, enokubopha ngokukhawuleza, i-adsorb, eyahlukileyo kwaye ihlambulule kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye icoceke kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye ihlambe kwaye icoceke kwaye ihlambe kwaye icoceke kwaye ihlambe kwaye ihlambe kwaye icoceke kwaye ihlambe kwaye ihlambe kwaye icoceke kwaye ihlambe kwaye ihlambe i-acid acid. Ilungele ukwahlula kunye nokucoca i-RNA kwizicubu nakwiiseli. Ngokuxhasa ukusetyenziswa kwe-Bignetish Bednetic Bead Acid I-Acleid I-Acceractor ye-Acleid, ilungele ukuthotyelwa okuzenzekelayo kweesayizi ezinkulu zesampulu. Imveliso ekhutshiweyo ye-nucleid inococeko oluphezulu kunye nomgangatho olungileyo, kwaye inokusetyenziswa ngokubanzi kwi-Dowstream RT-rtream RT-PRCR / RCRCR, i-NGS kunye nolunye uhlobo lovavanyo lovavanyo.

QHAGAMSHELANA NATHI

Iinkcukacha zemveliso

Iimpawu zeMveliso

Iimbonakalo

Uluhlu olubanzi lweesampulu:inokusetyenziswa ngokubanzi kwizicubu ezahlukeneyo zezilwanyana kunye neeseli ezikhulisiweyo

Ikhuselekile kwaye ingenabunzi:Akukho sidingo se-regents enetyhefu efana ne-phenol / chloroform, ikhuselekile kwaye ithembekile

Igalelo le-Automated Teemed-Imveliso:Ibhayisiwe nge-Beagle i-endelector ye-nucleid ye-nucleid, inokwenza ukhutshiwe okuphezulu kwaye ilungele ukukhulula ubungakanani besayizi enkulu

Ucoceko oluphezulu kunye nomgangatho olungileyo:Imveliso ekhutshiweyo icocekile kwaye inokusetyenziselwa uvavanyo lokuhlala oluhamba ezantsi njenge-RCR / RT QPR kunye ne-NG.

Qhelisela Izixhobo

I-Bigfish: I-BFEX-32E, I-Bfex-32, BFEX-16E,I-Bfex-96e

Ukuchazwa kwemveliso

ImvelisoNAme	Ikati. Hayi.	Ukupakisha
MagiPureTI-Otal RNAPgwebaKyona (Iphakheji yangaphambili)	Bfmp07r	32t
MagiPureTI-Otal RNAPgwebaKyona (Iphakheji yangaphambili)	Bfmp07r1	40t
MagiPureTI-Otal RNAPgwebaKyona (Iphakheji yangaphambili)	Bfmp07r96	I-96t
Dnase i (ukuthenga)	Bfrd009	1ml /tI-UBE(5u / il)