中文网站
Izicubu zezilwanyana zinokwahlulwa zibe zizicubu ze-epithelial, izicubu ezidibeneyo, izicubu ze-muscular kunye ne-neural tissues ngokwemvelaphi yazo, i-morphology, isakhiwo kunye neempawu eziqhelekileyo zokusebenza, ezidityanisiweyo kwaye zixhomekeke kwimilinganiselo eyahlukeneyo ukuze zenze iintlobo zamalungu kunye neenkqubo zezilwanyana ukwenzela ukugqiba imisebenzi eyahlukeneyo ye-physiological.
Izicubu ze-Epithelial: zenziwe ngeeseli ezininzi ze-epithelial ezicwangciswe ngokusondeleyo kunye nenani elincinci leeseli ze-interstitial zesakhiwo esifana ne-membrane, edla ngokugqunywa kumphezulu womzimba wesilwanyana kunye nomzimba weetyhubhu ezahlukeneyo, i-cavities, capsules kunye nomphezulu wangaphakathi wamalungu athile. Izicubu ze-Epithelial zinemisebenzi yokukhusela, ukufihla, ukukhupha kunye nokufunxa.
Izicubu ezidibeneyo: Iqulethwe ngamaseli kunye nenani elikhulu le-intercellular matrix. Izicubu ezidibeneyo eziveliswa yi-mesoderm yeyona nto isasazeke kakhulu kunye nokwahluka kwezicubu zezilwanyana, kubandakanya izicubu ezidibeneyo, izicubu ezixineneyo, izicubu ezixhumeneyo, i-cartilage, izicubu zethambo, izicubu ze-adipose njalo njalo. Inemisebenzi yokuxhasa, yokudibanisa, yokukhusela, yokukhusela, yokulungisa kunye nokuthutha.
Izicubu ze-muscle: zenziwe ngeeseli ze-muscle ezikwaziyo ukwenza isivumelwano. Imilo yeeseli zezihlunu ibhityile njengefayibha, ngoko ikwabizwa ngokuba yifayibha yezihlunu. Umsebenzi oyintloko we-muscle fiber kukukontraka kunye nokwenza ukunyakaza kwemisipha. Ngokwe-morphology kunye nesakhiwo seeseli ze-muscle kunye nemisebenzi eyahlukeneyo, izicubu ze-muscle zingahlulwa zibe yi-skeletal muscle (i-transverse muscle), i-muscle egudileyo kunye ne-cardiac muscle.
Izicubu zeNervous: Izicubu ezenziwe ngeeseli zemithambo-luvo kunye neeseli zeglial. Iiseli ze-nerve ziyi-morphological and functional units of the nervous system kwaye ziyakwazi ukuva i-stimuli yangaphakathi nangaphandle kwaye iqhube iimpembelelo kumzimba.
Imveliso yeBigfish
Imveliso ithatha inkqubo ye-buffer ekhethekileyo ephuhliswe ngokukodwa kwaye iphuculwe kwaye i-magnetic beads ibopha ngokukodwa i-DNA, enokuthi ibophe ngokukhawuleza kwaye ibhengeze, ihlukanise kwaye ihlambulule i-nucleic acids. Ifanelekile ukukhutshwa ngokufanelekileyo kunye nokuhlanjululwa kwe-DNA ye-genomic kuzo zonke iintlobo zezicubu zezilwanyana kunye nezitho zangaphakathi (kubandakanywa nezinto eziphilayo zaselwandle), kwaye inokususa ngokugqithiseleyo zonke iintlobo zeeprotheni, amafutha kunye nezinye izinto eziphilayo kunye nezinye izinto ezingcolileyo. Ingasetyenziswa kunye neIntlanzi enkuluI-Magnetic Bead Method Nucleic Acid Extractor, efanelekileyo kakhulu kwi-automated extraction yeesayizi ezinkulu zesampulu. Iimveliso ze-nucleic acid ezikhutshiweyo zicocekile kunye nomgangatho ophezulu, kwaye zinokusetyenziswa ngokubanzi kwi-PCR / qPCR esezantsi, i-NGS, i-hybridisation yaseMazantsi kunye nolunye uphando lovavanyo.
Iimbonakalo:
Uluhlu olubanzi lweesampulu: i-DNA ye-genomic inokukhutshwa ngokuthe ngqo kuzo zonke iintlobo zeesampulu zenyama yezilwanyana
Ikhuselekile kwaye ayinayo ityhefu: i-reagent ayinazo izinyibilikisi ezinetyhefu ezifana ne-phenol, i-chloroform, njl., kunye nokhuseleko oluphezulu.
I-automation: ukuhambelana neBigfish Nucleic Acid Extractor ingasetyenziselwa ukutsalwa okuphezulu, ngakumbi ilungele ukutsalwa kwesampulu enkulu.
Ukucoceka okuphezulu: ingasetyenziselwa imifuniselo yebhayoloji yemolekyuli, efana nePCR, ukugaya i-enzyme kunye nokuxutywa ngokuthe ngqo.
Izixhobo ezisebenzayo: BFEX-32/BFEX-32E/BFEX-96E
Inkqubo yokutsalwa:
Isampula: 25-30mg yezicubu zezilwanyana
Ukugaya: Ukusila initrogen engamanzi, ukucola okanye ukucheba
Ukwetyisa: 56℃ kwibhafu yokwetyisa efudumeleyo
Ukukhwela ibhodi: i-centrifugation ukususa i-supernatant, kwaye yongeze kwipleyiti enzulu yokutsalwa ebhodini.
Idatha yovavanyo: I-30mg yeesampulu zezicubu ezivela kwiindawo ezahlukahlukeneyo zegundane zithathwe kwaye i-DNA extraction kunye nokuhlanjululwa kwenziwa nge-BFMP01R ngokwemiyalelo. Iziphumo zovavanyo zibonise ukuba ikhithi ye-BFMP01R inezinga elilungileyo lokutsalwa.
Ixesha lokuposa: Jul-17-2025
Iisetingi zabucala