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Izicubu zezilwanyana zinokwahlulwa zibe zizicubu ze-epithelial, izicubu ezidibeneyo, izicubu zemisipha kunye nezicubu ze-neural ngokwemvelaphi yazo, imo, isakhiwo kunye neempawu ezifanayo zokusebenza, ezidibeneyo kwaye zixhomekeke kwelinye ngobuninzi obahlukeneyo ukwenza amalungu kunye neenkqubo ezahlukeneyo zezilwanyana ukuze kugqitywe imisebenzi eyahlukeneyo ye-physiological.
Izicubu ze-Epithelial: zenziwe ziiseli ezininzi ze-epithelial ezicwangciswe ngokusondeleyo kunye nenani elincinci leeseli eziphakathi kwesakhiwo esifana ne-membrane, ezihlala zigqunywe kumphezulu womzimba wesilwanyana kunye nomzimba weetyhubhu ezahlukeneyo, imingxunya, iikhaphu kunye nomphezulu wangaphakathi wamalungu athile. Izicubu ze-Epithelial zinemisebenzi yokukhusela, ukukhupha, ukukhupha kunye nokufunxa.
Izicubu ezidityanisiweyo: Zenziwe ziiseli kunye nenani elikhulu le-intercellular matrix. Izicubu ezidityanisiweyo eziveliswa yi-mesoderm lolona hlobo lwezicubu zezilwanyana ezisasazeke kakhulu nezihlukeneyo, kuquka izicubu ezidityanisiweyo ezikhululekileyo, izicubu ezidityanisiweyo ezixineneyo, izicubu ezidityanisiweyo ze-reticular, izicubu ze-cartilage, izicubu zamathambo, izicubu zamafutha njalo njalo. Zinemisebenzi yokuxhasa, ukunxibelelana, ukukhusela, ukuzikhusela, ukulungisa kunye nokuthutha.
Izicubu zemisipha: zenziwe ziiseli zemisipha ezikwaziyo ukufinyela. Imilo yeeseli zemisipha incinci njengefayibha, ngoko ke ikwabizwa ngokuba yifayibha yemisipha. Umsebenzi ophambili wefayibha yemisipha kukufinyela nokwenza intshukumo yemisipha. Ngokwemo kunye nolwakhiwo lweeseli zemisipha kunye nemisebenzi eyahlukeneyo, izicubu zemisipha zinokwahlulwa zibe yimisipha yamathambo (imisipha enqamlezileyo), imisipha egudileyo kunye nemisipha yentliziyo.
Izicubu zemithambo-luvo: izicubu ezakhiwe ziiseli zemithambo-luvo kunye neeseli ze-glial. Iiseli zemithambo-luvo ziiyunithi zemo kunye nezisebenzayo zenkqubo yemithambo-luvo kwaye zinamandla okuva izinto zangaphakathi nezangaphandle kunye nokuqhuba iimpumlo kwindalo.
Imveliso yeBigfish
Le mveliso isebenzisa inkqubo ye-buffer ekhethekileyo eyenzelwe ngokukodwa kwaye ilungiselelwe kwaye inee-magnetic beads ezibopha ngokukodwa i-DNA, enokubopha ngokukhawuleza nokufunxa, yahlule kwaye icoce ii-nucleic acids. Ifanelekile ekukhupheni nasekucoceni i-genomic DNA kuzo zonke iintlobo zezicubu zezilwanyana kunye nezitho zangaphakathi (kuquka izinto eziphilayo zaselwandle), kwaye inokususa zonke iintlobo zeeproteni, amafutha kunye nezinye izinto eziphilayo kunye nezinye izinto ezingcolileyo. Ingasetyenziswa kunyeIntlanzi enkuluIndlela yeMagnetic Bead Nucleic Acid Extractor, efanelekileyo kakhulu ekukhupheni ngokuzenzekelayo ubungakanani obukhulu beesampuli. Iimveliso ze-nucleic acid ezikhutshiweyo zicocekile kwaye zisemgangathweni ophezulu, kwaye zingasetyenziswa kakhulu kwi-downstream PCR/qPCR, NGS, Southern hybridisation kunye nolunye uphando lovavanyo.
Iimbonakalo:
Uluhlu olubanzi lweesampuli: i-DNA ye-genomic inokukhutshwa ngokuthe ngqo kuzo zonke iintlobo zeesampuli zezicubu zezilwanyana
Ikhuselekile kwaye ayinabungozi: i-reagent ayinazo izinyibilikisi ezinobuthi ezifana ne-phenol, i-chloroform, njl.njl., ezinokhuseleko oluphezulu
Ukuzenzekela: ukufanisa neBigfish Nucleic Acid Extractor kungasetyenziselwa ukukhutshelwa okuphezulu, ngakumbi okufanelekileyo ekukhutshelweni kwesampulu enkulu
Ubumsulwa obuphezulu: ingasetyenziselwa uvavanyo lwebhayoloji yemolekyuli, olufana ne-PCR, ukugaya ii-enzyme kunye nokudityaniswa ngokuthe ngqo
Izixhobo Ezisebenzayo: BFEX-32/BFEX-32E/BFEX-96E
Inkqubo yokukhupha:
Ukuthatha iisampulu: 25-30mg yezicubu zezilwanyana
Ukusila: ukusila i-nitrogen engamanzi, ukusila okanye ukucheba
Ukugaya: 56℃ ukugaya okufudumeleyo ebhafini
Ukufakwa kwindawo yokubeka: ukucoca i-centrifugation ukususa i-supernatant, kunye nokongeza kwipleyiti yomthombo onzulu ukuze kukhutshwe kwindawo yokubeka.
Idatha yovavanyo: Iisampulu zezicubu ezingama-30mg ezivela kwiindawo ezahlukeneyo zeempuku zithathwe kwaye ukukhutshwa kwe-DNA kunye nokucocwa kwenziwa nge-BFMP01R ngokwemiyalelo. Iziphumo zovavanyo zibonise ukuba ikhithi ye-BFMP01R inesantya esihle sokukhupha.
Ixesha leposi: Julayi-17-2025
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