Ukukhawuleza nokusulungekiswa komhlaba, kulula ukutsalwa kweDNA yelindle ngolandelelwano lweentlanzi ezinkulu

Umhlaba, njengendalo engqongileyo eyahlukeneyo, utyebile kwimithombo ye-microbial, kubandakanywa uluhlu olubanzi lweentlobo ze-microbial ezifana nebhaktiriya, umngundo, iintsholongwane, i-cyanobacteria, i-actinomycetes, i-protozoa kunye ne-nematodes. Ukuba noluhlu olubanzi lwemisebenzi ye-metabolic kunye neempawu zomzimba, zidlala indima ebalulekileyo kumjikelezo wezondlo zomhlaba kwaye zibalulekile ekususeni ukungcola komhlaba. Njengenye yezona ndawo zihlukeneyo ngokwebhayoloji emhlabeni, izifundo zebhayoloji yemolekyuli yomhlaba zibalulekile kwibhayoloji eyodwa. Kule nkqubo, ukufumana i-microbial DNA kwiisampulu zomhlaba linyathelo lokuqala kuphando lomhlaba kunye nelona nyathelo libalulekileyo kwimpumelelo yovavanyo olusezantsi. Nangona kunjalo, ukongeza kwimithombo ecebileyo ye-microbial, umhlaba uhlala uqulethe inani elikhulu le-metabolites (i-humic acid, i-xanthic acid kunye nezinye izinto ze-humic), ezinokucocwa ngokulula kunye ne-nucleic acids ngexesha lenkqubo yokukhupha i-nucleic acid, echaphazela i-PCR esezantsi kunye nenkqubo yokulandelelana.Intlanzi enkuluUkulandelelana koMhlaba kunye ne-Faecal Genomic DNA ye-Genomic Purification Kit inokukhupha i-DNA esulungekileyo negxininiswe kakhulu ye-genomic kwiisampulu ezityebileyo ze-humus ezifana nelindle lomhlaba, engumncedi onamandla wophando ngeentlobo ngeentlobo zezinto eziphilayo emhlabeni.