Ukukhupha i-DNA yomhlaba/ilindle ngokukhawuleza nangokucocekileyo nangokulula ngeBig Fish Sequence

Umhlaba, njengendawo enezinto ezahlukeneyo zendalo, utyebile kwizixhobo ze-microbial, kuquka iintlobo ezahlukeneyo ze-microbial ezifana neebhaktheriya, iifungi, iintsholongwane, i-cyanobacteria, i-actinomycetes, i-protozoa kunye ne-nematodes. Zineentlobo ngeentlobo zemisebenzi ye-metabolic kunye neempawu ze-physiological, zidlala indima ebalulekileyo ekujikelezeni kwezakhamzimba zomhlaba kwaye zibalulekile ekususeni ungcoliseko lomhlaba. Njengenye yezona ndawo zininzi zebhayoloji emhlabeni, izifundo zebhayoloji ye-molecular yomhlaba zibaluleke kakhulu kwibhayoloji. Kule nkqubo, ukufumana i-DNA ye-microbial kwiisampuli zomhlaba linyathelo lokuqala kuphando lomhlaba kwaye lelona nyathelo libalulekileyo kwimpumelelo yovavanyo oluya ezantsi. Nangona kunjalo, ukongeza kwizixhobo ze-microbial ezityebileyo, umhlaba uhlala uqulathe inani elikhulu lee-metabolites (i-humic acid, i-xanthic acid kunye nezinye izinto ze-humic), ezinokucocwa ngokulula kunye nee-nucleic acid ngexesha lenkqubo yokukhupha i-nucleic acid, echaphazela inkqubo ye-PCR esezantsi kunye nokulandelelana.Intlanzi EnkuluI-Sequencing Soil and Fecal Genomic DNA Purification Kit inokukhupha i-DNA ye-genomic ecocekileyo nexineneyo kwiisampulu ezityebileyo ze-humus ezifana nendle yomhlaba, nto leyo eluncedo kakhulu kuphando lokwahlukahlukana kwe-microbial ecosystem yomhlaba.

Imveliso yeNtlanzi Enkulu

Le mveliso isebenzisa inkqubo ye-buffer ekhethekileyo eyenziwe ngokukodwa nelungiselelwe kakuhle kunye nee-magnetic beads ezibopha ngokukodwa i-DNA, enokubopha nokufunxa ngokukhawuleza, yahlule kwaye icoce ii-nucleic acids, okwenza ukuba ilungele ukwahlulwa ngokukhawuleza nangokufanelekileyo kunye nokucocwa kwe-genomic DNA emhlabeni nakwindle, kunye nokususwa kweentsalela ezifana nee-humic acids, iiproteni, ii-ion zetyuwa, kunye nezinye iintsalela. Ngokuhambelana ne-Beaglefly sequencing magnetic bead method nucleic acid extractor, ifanelekile kakhulu ekukhutshweni ngokuzenzekelayo kobukhulu besampulu enkulu. I-genomic DNA ekhutshiweyo icocekile kwaye isemgangathweni kwaye ingasetyenziswa kakhulu kwi-downstream PCR/qPCR, NGS nakwezinye uphando lovavanyo.

Iimbonakalo

Umgangatho olungileyo:ukwahlulwa kunye nokucocwa kwe-DNA ye-genomic, isivuno esiphezulu, ubunyulu obuhle.

Uluhlu olubanzi lweesampuli:ingasetyenziswa kakhulu kuzo zonke iintlobo zesampulu zomhlaba kunye nelindle.

Ngokukhawuleza kwaye kulula:ukukhupha ngokuzenzekelayo nge-extractor efanayo, efanelekileyo ngakumbi ekukhupheni ubungakanani obukhulu beesampuli.

Ikhuselekile kwaye ayinabungozi:akukho mfuneko yee-reagents ze-organic ezinobungozi ezifana ne-phenol/chloroform, njl.njl.

Izixhobo ezilungisekayo:I-BFEX-32/ BFEX-32E/ BFEX-96E


Ixesha leposi: Julayi-10-2025
Useto lwabucala
Lawula Imvume Yekhukhi
Ukuze sinike amava angcono kakhulu, sisebenzisa ubuchwepheshe obufana neekuki ukugcina kunye/okanye ukufikelela kulwazi lwesixhobo. Ukuvuma obu buchwepheshe kuya kusivumela ukuba sicubungule idatha efana nokuziphatha kokukhangela okanye ii-ID ezizodwa kule sayithi. Ukungavumi okanye ukurhoxisa imvume, kunokuchaphazela kakubi iimpawu kunye nemisebenzi ethile.
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