I-Spamictoure yesilwanyana se-tissic gemickic jomic cat

I-alfawal Alfaltis Tissue Sunty DNA Ukuhlanjwa kweeKhowudi

Inkcazo emfutshane:

Le mveliso isebenzisa i-Buffer ekhethekileyo kunye ne-Buffer ekhethekileyo ye-buffer kunye neebhlorho zemagnethi ezibophelele ngqo iDNA. Inokukhawulezisa ngokukhawuleza, i-adsorb, yahlukile kwaye ihlambulule i-acid acid. Ilungele ukufumana i-DNA efanelekileyo kwaye icoce iDNA ye-gemic evela kwizicubu ezahlukeneyo zezilwanyana kunye namalungu angaphakathi (kubandakanya izinto zaselwandle). Inokususa ukungcola ezinje ngeeproteni ezahlukeneyo, amafutha kunye nezinye izinto eziphilayo ukuya kuthi ga kwinqanaba elikhulu. Ixhotywe nge-Bignetish Bead Bead Storemes isixhobo sokukhuphela, ilungele ukuthunyelwa ngokuzenzekelayo kwimiqulu emikhulu yesampulu. Iimveliso ezikhutshiweyo ze-nucleidi ezikhutshiweyo zinococeko oluphezulu kunye nomgangatho olungileyo, kwaye zinokusetyenziswa ngokubanzi kwi-DOWSTREAM PCR / QPCR, i-NGS, i-Earger Sorks kunye nolunye uphando lovavanyo.

QHAGAMSHELANA NATHI

Iinkcukacha zemveliso

Iimpawu zeMveliso

Iimpawu zemveliso

Uluhlu olubanzi lwezicelo zesampulu:I-DNA ye-gemic inokuthi ikhutshwe ngokuthe ngqo kwiisampulu zezilwanyana ezahlukeneyo
Ikhuselekile kwaye ingenabunzi:I-Regent ayinazo iityhefu ezinetyhefu ezinje nge-phenol kunye ne-chloroform, kwaye inezinto ezikhuselekileyo zokhuseleko.
Autous:I-Acid ye-Acid ye-Acid ye-nucleid ye-nucleid ye-nucleid ye-nucleid inokwenza ukhuphelo lwegalelo eliphezulu, ngakumbi ilungele ukuthotyelwa kwesampulu enkulu
Ucoceko oluphezulu:Ingasetyenziswa ngokuthe ngqo kwi-PCR, i-enzyme yokugaya, i-hybridation kunye nezinye iimolekyuli zemolekyuli

Iinkqubo zokukhupha

I-Mangadapure-Tishu-tishu-tishu-tissic-tissic-dna-ikhithi

Imifanekiso yezilo yezilwanyana-Grinder kunye neMifanekiso ye-Anir - I-Anil Back imifanekiso-i-NulecIic Acid
ISAMPELA:Thatha i-25-30mg yezilwanyana
Ukugaya:I-nitrogen ye-nitrogen, grinder yokugaya okanye ukusika
Ukugaya:I-56 ℃ Diginotion efudumeleyo
Kumatshini:I-Centrifuge kwaye uthathe i-supernatant, yongeza kwipleyiti enzulu kwaye ikhuphe kumatshini

Iiparamitha zobugcisa

Isampulu:25-30mg
I-DNA Pemponity:A260 / 280 ≧ 1.75

Isixhobo esifanelekileyo

I-Bigfish Bfex-32 / BFEX-32E / BFEX-96E

Ukucaciswa kwemveliso

Igama lemveliso	Ikati.No.	Ukupakisha
I-Spaminal Assal I-Tusic Sunty Pission Deking Kikhi yeKit (iphakheji yangaphambili)	Bfmp01r	32t
I-Spaminal Assal I-Tusic Sunty Pission Deking Kikhi yeKit (iphakheji yangaphambili)	Bfmp01R1	40t
I-Spaminal Assal I-Tusic Sunty Pission Deking Kikhi yeKit (iphakheji yangaphambili)	I-BFMP01R96	I-96t
Rnase a (ukuthenga)	Bfrd017	I-1ml / PC (i-10mg / ml)