I-MagPure Bacteria yeGenomic DNA Yokucoca Ikhithi

Inkcazelo emfutshane:

Emva kokunyangwa ngolwelo lokwetyisa, i-nucleic acid kwisampulu yebhaktheriya ikhutshwa kuphela kusetyenziswa iLysis Buffer. I-DNA ekhutshiweyo ye-genomic ibotshelelwe ngokukodwa kwaye ngokukodwa amaso eMagnetic. I-DNA ye-genomic ebotshelelwe kwiincinci zemagneti ibanjwe ngezinto eziphathekayo; izingcolisi ziyasuswa ngokuhlanjwa nge-Wash Buffer. I-asidi ye-nucleic iye yakhutshwa kumasuntswana nge-Elution Buffer. Isebenza kuzo zonke iintlobo zeebhaktheriya zegram-negative kunye neebhaktheriya ezintle, njl.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Icandelo eliphambili

Ikati.#	I-BFMP10R1	I-BFMP10R16	I-BFMP10R	Cabachasi
Paukutsiba	1T40T/ikhithi	16T16T/ikhithi	32T32T/ikhithi
UProtease K	400μ L x 2	320μ L	640μ L	Isisombululo seProtease (Sipakishwe ngokwahlukeneyo)
RNaseA	80μL	32μ L	64μL	Isisombululo se-enzyme (Sipakishwe ngokwahlukeneyo)
Buffer BA	12mL	5ml	10ml	Isisombululo saline
Isithinteli BL	Kumashumi amane anesithandathu-kakuhle ukulungiselela. ikhatriji 40 iipcs.	96 kakuhle ipakishwe kwangaphambili ipleyiti iipcs ezi-2.	96 kakuhle ipakishwe kwangaphambili ipleyiti iipcs ezi-2.	I-denaturant eyomeleleyo kunye ne-Tris buffer
Buffer WA				Isisombululo saline
Buffer WB				Isisombululo se-saline ephantsi
Isithinteli DE				Tris isisombululo
Iintsimbi zeMagnetic				Isisombululo se-Hydroxy magnetic bead
Incwadi yabasebenzisi	1	1	1