I-MagPure Bacteria yeGenomic DNA Yokucoca Ikhithi

Inkcazo emfutshane:

Emva kokunyangwa ngolwelo lokugaya ukutya, i-nucleic acid kwisampulu yebhaktheriya ikhutshwa kuphela kusetyenziswa iLysis Buffer. I-genomic DNA ekhutshiweyo ibotshelelwa ngokukodwa kwiMagnetic beads. I-genomic DNA ebotshelelwe kwi-magnetic particles ibanjwa ngezinto zemagnethi; izinto ezingcolisayo zisuswa ngokuhlanjwa ngeWash Buffer. I-nucleic acid emva koko isuswa kwi-particles nge-Elution Buffer. Isebenza kuzo zonke iintlobo zebhaktheriya ezine-gram-negative kunye nebhaktheriya ezintle, njl.

QHAGAMSHELANA NATHI

Iinkcukacha zeMveliso

Iithegi zeMveliso

Icandelo eliphambili

Ikati.#	I-BFMP10R1	I-BFMP10R16	I-BFMP10R	Cizinto
Pai-cking	1T40T/ikhithi	16T16T/ikhithi	32T32T/ikhithi
IProtease K	400μ L x 2	320μ L	640μ L	Isisombululo seProtease (Sipakishwe ngokwahlukeneyo)
I-RNaseA	80μ L	32μ L	64μL	Isisombululo se-enzyme (Sipakishwe ngokwahlukeneyo)
I-BA ye-Buffer	12mL	5ml	10ml	Isisombululo setyuwa
I-Buffer BL	Kwi-40-well ukulungiselela. ikhatriji 40 ii-pcs.	96 iqula ipakishwe kwangaphambili ipleyiti 2 ii-pcs.	96 iqula ipakishwe kwangaphambili ipleyiti 2 ii-pcs.	I-denaturant enamandla kunye ne-Tris buffer
I-Buffer WA				Isisombululo setyuwa
I-Buffer WB				Isisombululo se-saline esiphantsi
I-Buffer DE				Isisombululo seTris
Iibhondi zeMagnetic				Isisombululo se-hydroxy magnetic bead
Incwadi Yabasebenzisi	1	1	1